Skip to main content
Log in

Rapid detection of Campylobacter jejuni using fluorescent microspheres as label for immunochromatographic strip test

  • Research Article
  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

Abstract

Campylobacter jejuni is a worldwide foodborne pathogen recognized as a leading cause of human gastrointestinal enteritis. A rapid, sensitive, and specific method is required to monitor food and water in cases of contamination by this pathogen. This report presents a novel immunochromatographic test (ICT) using fluorescent microspheres labeled with polyclonal antibodies of C. jejuni as the capture reagent dispensed onto the conjugate pad. Polyclonal antibodies against the outer membrane protein PEB1 of C. jejuni were used as the detective reagent at the test line, whereas the goat anti-rabbit IgG was used on the control line. PEB1 was obtained by gene cloning and expression to prepare its antibody. In this study, a simple and rapid ICT is reported for detecting C. jejuni for the first time with a detection limit of 106 CFU/ mL.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Tauxe RV. Incidence, trends, and sources of campylobacteriosis in developed countries: An overview. pp. 42–43. In: The Increasing Incidence of Human Campylobacteriosis. Report and proceedings of a WHO consultation of experts. (WHO/CDS/CSR/APH 2001.7) World Health Organization, Cophenhagen, Denmark (2001)

    Google Scholar 

  2. EFSA (European Food Safety Agency). The community summary report on trends and sources of zoonoses, zoonotic agents in the European Union in 2007. J. EFSA 223: 223–226 (2009)

    Google Scholar 

  3. Bardon J, Kolar M, Cekanova L, Hejnar P, Koukalova D. Prevalence of Campylobacter jejuni and its resistance to antibiotics in poultry in the Czech Republic. Zoonoses Public Hlth. 56: 111–116 (2009)

    Article  CAS  Google Scholar 

  4. Tauxe RV. Epidemiology of Campylobacter jejuni infections in the United States and other industrialized nations. pp. 9–19. In: Campylobacter jejuni Current Status and Future Trends. Nachamkin I, Blaser M, Tompkins L. (eds). American Society for Microbiology, Washington, DC, USA (1992)

    Google Scholar 

  5. Habib I, Sampers I, Uyttendaele M, Berkvens D, Zutter L. Performance characteristics and estimation of measurement uncertainty of three plating procedures for Campylobacter enumeration in chicken meat. Int. J. Food Microbiol. 25: 65–74 (2008)

    Article  CAS  Google Scholar 

  6. LaGier MJ, Joseph LA, Passaretti TV, Musser KA, Cirino NM. A real-time multiplexed PCR assay for rapid detection and differentiation of Campylobacter jejuni and Campylobacter coli. Mol. Cell. Probe. 18: 275–282 (2004)

    Article  CAS  Google Scholar 

  7. Wang G, Clark CG, Taylor TM, Pucknell C, Barton C, Price L, Woodward DL, Rodgers FG. Colony multiplex PCR assay for identification and differentiation of Campylobacter jejuni, C. coli, C. lari, C. upsaliensis, and C. fetus subsp. fetus. J. Clin. Microbiol. 40: 4744–4747 (2002)

    Article  CAS  Google Scholar 

  8. Burnett TA, Hornitzky MA, Kuhnert P, Djordjevic SP. Speciating Campylobacter jejuni and Campylobacter coli isolates from poultry and humans using six PCR-based assays. FEMS Microbiol. Lett. 216: 201–209 (2002)

    Article  CAS  Google Scholar 

  9. Wei D, Oyarzabal OA, Huang TS, Balasubramanian S, Sista S, Simonian AL. Development of a surface plasmon resonance biosensor for the identification of Campylobacter jejuni. J. Microbiol. Meth. 69: 78–85 (2007)

    Article  CAS  Google Scholar 

  10. Kanki M, Sakata J, Taguchi M, Kumeda Y, Ishibashi M, Kawai T, Kawatsu K, Yamasaki W, Inoue K, Miyahara M. Effect of sample preparation and bacterial concentration on Salmonella enterica detection in poultry meat using culture methods and PCR assaying of preenrichment broths. Int. J. Food Microbiol. 26: 1–3 (2009)

    Article  CAS  Google Scholar 

  11. Fauchere JL, Kervella M, Rosenau A, Mohanna K, Veron M. Adhesion to HeLa cells of Campylobacter jejuni and C. coli outer membrane components. Res. Microbiol. 140: 379–392 (1989)

    Article  CAS  Google Scholar 

  12. Pei Z, Blaser MJ. PEB1, the major cell-binding factor of Campylobacter jejuni, is a homolog of the binding component in Gram-negative nutrient transport systems. J. Biol. Chem. 268: 18717–18725 (1993)

    CAS  Google Scholar 

  13. Du LF, Li ZJ, Tang XY, Huang JQ, Sun WB. Immunogenicity and immunoprotection of recombinant PEB1 in Campylobacter jejuni-infected mice. World J. Gastroenterol. 14: 6244–6248 (2008)

    Article  CAS  Google Scholar 

  14. Kawatsu K, Kumeda Y, Taguchi M, Yamazaki-Matsune W, Kanki M, Inoue K. Development and evaluation of immunochromatographic assay for simple and rapid detection of Campylobacter jejuni and Campylobacter coli in human stool specimens. J. Clinteral. Microbiol. 46: 1226–1231 (2008)

    Article  CAS  Google Scholar 

  15. Sharp JM, Doran PM. Strategies for enhancing monoclonal antibody accumulation in plant cell and organ cultures. Biotechnol. Progr. 17: 979–992 (2001)

    Article  CAS  Google Scholar 

  16. Bibila TA, Robinson DK. In pursuit of the optimal fed-batch process for monoclonal antibody production. Biotechnol. Progr. 11: 1–13 (1995)

    Article  CAS  Google Scholar 

  17. Farboud B, Aotaki KA, Miyata T, Hjelmeland LM, Handa JT. Development of a polyclonal antibody with broad epitope specificity for advanced glycation endproducts and localization of these epitopes in Bruch’s membrane of the aging eye. Mol. Vis. 5: 11–15 (1999)

    CAS  Google Scholar 

  18. Bradley M, Ashokkumar M, Grieser F. Sonochemical production of fluorescent and phosphorescent latex particles. J. Am. Chem. Soc. 125: 525–529 (2003)

    Article  CAS  Google Scholar 

  19. Endtz H, Ang C, Van Den Braak N, Luijendijk A, Jacobs B, De Man P, Van Duin J, Van Belkum A, Verbrugh H. Evaluation of a new commercial immunoassay for rapid detection of Campylobacter jejuni in stool samples. Eur. J. Clin. Microbiol. 19: 794–797 (2000)

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Feng Xu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xu, D., Wu, X., Li, B. et al. Rapid detection of Campylobacter jejuni using fluorescent microspheres as label for immunochromatographic strip test. Food Sci Biotechnol 22, 585–591 (2013). https://doi.org/10.1007/s10068-013-0118-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10068-013-0118-5

Keywords

Navigation