Food Science and Biotechnology

, Volume 22, Issue 2, pp 577–584 | Cite as

Efficiency of fractionated γ-irradiation doses to eliminate vegetative cells and spores of Bacillus cereus from raw rice

  • Adelard B. Mtenga
  • Neema Kassim
  • Won-Bo Shim
  • Duck-Hwa Chung
Research Article


Efficacy of fractionated γ-irradiation to eliminate vegetative and spore forms of Bacillus cereus from raw rice was studied. Viable bacteria and spores count performed after irradiation treatment revealed that vegetative cells and spores (7.9 and 7.7 log CFU/g) of B. cereus in raw rice tolerated γ-irradiation up to 10 and 20 kGy, respectively and were eliminated at 15 and 25 kGy respectively on single treatment. Exactly 2 times of 5 kGy irradiation treatment eliminated all vegetative B. cereus (7.9 log CFU/g). A treatment with fractionated doses of γ-irradiation effectively eliminated vegetative bacteria but not spores of B. cereus. Field emission SEM images revealed the damage by γ-irradiation to the spore exosporium. This study suggests new approach of using fractionated doses of γ-irradiation to eliminate foodborne pathogens in food which are affected by high doses of γ-irradiation.


fractionated γ-irradiation Bacillus cereus spore vegetative cell 


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  1. 1.
    World Health Organization. Food safety and foodborne illness. Fact sheet Number 237, Reviewed March 2007. World Health Organization, Media centre (2011). Available from: Accessed Feb. 12, 2012.Google Scholar
  2. 2.
    Gwack J, Lee KC, Lee HJ, Kwak W, Lee DW, Choi, YH, Kim JS, Kang YA. Trends in water- and foodborne disease outbreaks in Korea 2007–2009. Public Health Res. Perspect. 1: 50–54 (2010)CrossRefGoogle Scholar
  3. 3.
    Horchner PM, Pointon AM. HACCP-based program for on-farm food safety for pig production in Australia. Food Control 22: 1674–1688 (2011)CrossRefGoogle Scholar
  4. 4.
    Todd ECD, Michaels BS, Smith D, Greig JD, Bartleson CA. Outbreaks where food workers have been implicated in the spread of foodborne disease. Part 9. Washing and drying of hands to reduce microbial contamination. J. Food Protect. 73: 1937–1955 (2010)Google Scholar
  5. 5.
    Mendonca AF, Romero MG, Lihono MA, Nannapeneni R, Johnson MG. Radiation resistance and virulence of Listeria monocytogenes Scott A following starvation in physiological saline. J. Food Protect. 67: 470–474 (2004)Google Scholar
  6. 6.
    Song HP, Kim DH, Jo C, Lee CH, Kim KS, Byun MW. Effect of gamma irradiation on the microbiological quality and antioxidant activity of fresh vegetable juice. Food Microbiol. 23: 372–378 (2006)CrossRefGoogle Scholar
  7. 7.
    World Health Organization and Food and Agriculture Organization, 1991. Food irradiation: A technique for preserving and improving the safety of food. Geneva: WHO, (revised). Available from: Accessed Feb. 14, 2012.Google Scholar
  8. 8.
    SarrÌas JA, Valero M, Salmerón MC. Elimination of Bacillus cereus contamination in raw rice by electron beam irradiation. Food Microbiol. 20: 327–332 (2003)CrossRefGoogle Scholar
  9. 9.
    Mtenga AB, Kassim N, Lee WG, Shim WB, Yoon Y, Chung DH. Resistance of Bacillus cereus and its enterotoxin genes in ready-toeat foods to γ-irradiation. Food Sci. Biotechnol. 21: 443–452 (2012)CrossRefGoogle Scholar
  10. 10.
    Setlow P. Spores of Bacillus subtilis: Their resistance to and killing by radiation, heat, and chemicals. J. Appl. Microbiol. 101: 514–525 (2006)CrossRefGoogle Scholar
  11. 11.
    Spotheim-Maurizota M, Davídkováb M. Radiation damage to DNA in DNA-protein complexes. Mutat. Res. 711: 41–48 (2011)CrossRefGoogle Scholar
  12. 12.
    World Health Organization. High-dose irradiation: Wholesomeness of food irradiated with doses above 10 kGy. Technical Report Series no. 890. WHO, Geneva. (1999) Available from: Accessed Feb. 14, 2012.Google Scholar
  13. 13.
    Johnson KM. Bacillus cereus food-borne illness. An update. J. Food Protect. 47: 145–153 (1984)Google Scholar
  14. 14.
    Arnesen LPS, Fagerlund A, Granum PE. From soil to gut: Bacillus cereus and its food poisoning toxins. Fems Microbiol. Rev. 32: 579–606 (2008)CrossRefGoogle Scholar
  15. 15.
    Ehling-Schulz M, Fricker M, Scherer S. Bacillus cereus, the causative agent of an emetic type of food-borne illness. Mol. Nutr. Food Res. 48: 479–487 (2004)CrossRefGoogle Scholar
  16. 16.
    Kramer JM, Gilbert RJ. Bacillus cereus and other Bacillus species. pp. 21–77. In: Foodborne Bacterial Pathogens. Doyle MP (ed). Marcel Dekker, New York, NY, USA (1989)Google Scholar
  17. 17.
    Kramer JM, Gilbert RJ. Bacillus cereus gastroenteritis. Vol. vii, pp. 119–153. In: Food Poisoning: Handbook of Natural Toxins. Tu AT (ed). Marcel Dekker, NewYork, NY, USA (1992)Google Scholar
  18. 18.
    Kotiranta A, Lounatmaa K, Haapasalo M. Epidemiology and pathogenesis of Bacillus cereus infections. Microbes Infect. 2: 189–198 (2000)CrossRefGoogle Scholar
  19. 19.
    Nicholson WL, Munakata N, Horneck G, Melosh HJ, Setlow P. Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments. Microbiol. Mol. Biol. R. 64: 548–572 (2000)CrossRefGoogle Scholar
  20. 20.
    SarrÌas J, Valero A, Salmerón MC. Enumeration, isolation, and characterization of Bacillus cereus strains from Spanish raw rice. Food Microbiol. 19: 589–595 (2002)CrossRefGoogle Scholar
  21. 21.
    Granum PE. Bacillus cereus. pp. 373–381. In: Food Microbiology: Fundamentals and Frontiers. Doyle MP (ed). ASM Press, Washington, DC, USA (2001)Google Scholar
  22. 22.
    De Lara J, Ferná Ndez PS, Periago PM, Palop A. Irradiation of spores of Bacillus cereus and Bacillus subtilis with electron beams innovative. Food Sci. Emerg. Technol. 3: 379–384 (2002)CrossRefGoogle Scholar
  23. 23.
    Préstamo G, Pedrazuela A, Guignon B, Sanz PD. Synergy between high-pressure, temperature, and ascorbic acid on the inactivation of Bacillus cereus. Eur. Food Res. Technol. 225: 693–698 (2007)CrossRefGoogle Scholar
  24. 24.
    Fernández A, Ocio MJ, Fernández PS, Rodrigo M, MartÌnez A. Application of nonlinear regression analysis to the estimation of kinetic parameters for two enterotoxic strains of Bacillus cereus spores. Food Microbiol. 16: 607–613 (1999)CrossRefGoogle Scholar
  25. 25.
    Shin SY, Bajpai VK, Kim HR, Kang SC. Antibacterial activity of eicosapentaenoic acid (EPA) against foodborne and food spoilage microorganisms. LWT-Food Sci. Technol. 40: 1515–1519 (2007)CrossRefGoogle Scholar
  26. 26.
    Kirkman TW. 1996 Statistics to Use. Available from: Accessed Jan. 2, 2012.
  27. 27.
    Kim HJ, Ham JS, Lee JW, Kim K, Ha SD, Jo C. Effects of gamma and electron beam irradiation on the survival of pathogens inoculated into sliced and pizza cheeses. Radiat. Phys. Chem. 79: 731–734 (2010)CrossRefGoogle Scholar
  28. 28.
    Moini S, Tahergorabi R, Hosseini SV, Rabbani M, Tahergorabi Z, Feás X, Aflaki F. Effect of gamma radiation on the quality and shelf life of refrigerated rainbow trout (Oncorhynchus mykiss) fillets. J. Food Protect. 72: 1419–1426 (2009)Google Scholar
  29. 29.
    Trigo MJ, Sousa MB, Sapata MM, Ferreira A, Curado T, Andrada L, Botelho ML, Veloso MG. Radiation processing of minimally processed vegetables and aromatic plants. Radiat. Phys. Chem. 78: 659–663 (2009)CrossRefGoogle Scholar
  30. 30.
    Thayer DW, Boyd G, Fox JB, Lakritz L, Hampson JW. Variations in radiation sensitivity of foodborne pathogens associated with the suspending meat. J. Food Sci. 60: 63–67 (1995)CrossRefGoogle Scholar
  31. 31.
    Monk JD, Clavero RS, Beuchat LR, Doyle MP, Brackett RE. Irradiation inactivation of Listeria monocytogenes and Staphylococcus aureus in low- and high-fat, frozen, and refrigerated ground beef. J. Food Protect. 57: 969–974 (1994)Google Scholar
  32. 32.
    Thayer DW, Boyd G. Effect of irradiation temperature on inactivation of Escherichia coli O157:H7 and Staphylococcus aureus. J. Food Protect. 64: 1624–1626 (2001)Google Scholar
  33. 33.
    Glidewell SM, Deighton N, Goodman BA, Hillman JR. Detection of irradiated food: A review. J. Sci. Food Agr. 61: 281–300 (1993)CrossRefGoogle Scholar
  34. 34.
    Thakur BR, Singh RK. Food irradiations chemistry and applications. Food Rev. Int. 10: 437–473 (1994)CrossRefGoogle Scholar
  35. 35.
    Grecz N, Rowley DB, Matsuyama A. The action of radiation on bacteria and viruses. Vol. ii, pp. 167–218. In: Preservation of Food by Ionizing Radiation. Josephson ES, Peterson MS (eds). CRC Press, Boca Raton, FL, USA (1983)Google Scholar
  36. 36.
    Trampuz A, Piper KE, Steckelberg JM, Patel R. Effect of gamma irradiation on viability and DNA of Staphylococcus epidermidis and Escherichia coli. J. Med. Microbiol. 55: 1271–1275 (2006)CrossRefGoogle Scholar

Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Adelard B. Mtenga
    • 1
  • Neema Kassim
    • 1
  • Won-Bo Shim
    • 2
  • Duck-Hwa Chung
    • 1
  1. 1.Division of Applied Life Science (BK 21 Program)Gyeongsang National UniversityJinju, GyeongnamKorea
  2. 2.School of Physics and ChemistryGwangju Institute of Science and TechnologyGwangjuKorea

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