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Purification of antioxidative peptide from peptic hydrolysates of Mideodeok (Styela clava) flesh tissue

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Abstract

In this study, an antioxidative peptide was obtained by hydrolyzation of mideodeok (Styela clava) flesh tissue with various proteases and purified through gel filtration chromatography and reverse phage-HPLC (RPHPLC), and then antioxidant activity was investigated using electron spin resonance (ESR) spectrometer. Among the enzymatic hydrolysates, the peptic hydrolysate exhibited the highest antioxidant activity, and a strong antioxidant peptide was purified from the hydrolysate. The peptide sequence, Leu-Trp-His-Thr-His (692.2 Da), was identified by quardruple time-of-flight electrospray ionization (QTOF ESI)-MS. This antioxidative peptide strongly scavenged peroxyl radical with the IC50 value of 39.4 μM.

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Authors and Affiliations

  1. Department of Marine Life Sciences, Jeju National University, Jeju, 690-756, Korea

    Nalae Kang, Seok-Chun Ko, Kalpa Samarakoon, Eun-A Kim, Min-Cheol Kang & You-Jin Jeon

  2. Division of Food Science and Technology, Kyungnam University, Masan, Gyeongnam, 631-701, Korea

    Seung-Cheol Lee

  3. Department of Food Science and Nutrition, Pukyong National University, Busan, 608-737, Korea

    Jaeil Kim

  4. Department of Food Science and Biotechnology, Kunsan National University, Gunsan, Jeonbuk, 573-701, Korea

    Yong-Tae Kim

  5. Department of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University, Tongyeong, Gyeongnam, 650-160, Korea

    Jin-Soo Kim

  6. Deparment of Bioengineering at the Postgraduate School, Konkuk University, Seoul, 143-701, Korea

    Hyeran Kim

  7. Aqua Green Technology Co., Jeju Bio-Industry Center, Jeju, 690-121, Korea

    Kalpa Samarakoon & You-Jin Jeon

Authors
  1. Nalae Kang
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  2. Seok-Chun Ko
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  3. Kalpa Samarakoon
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  4. Eun-A Kim
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  7. Jaeil Kim
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  8. Yong-Tae Kim
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  9. Jin-Soo Kim
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  11. You-Jin Jeon
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Correspondence to You-Jin Jeon.

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Kang, N., Ko, SC., Samarakoon, K. et al. Purification of antioxidative peptide from peptic hydrolysates of Mideodeok (Styela clava) flesh tissue. Food Sci Biotechnol 22, 541–547 (2013). https://doi.org/10.1007/s10068-013-0112-y

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  • DOI: https://doi.org/10.1007/s10068-013-0112-y

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