Food Science and Biotechnology

, Volume 22, Issue 2, pp 485–491 | Cite as

Polymeric procyanidin fraction from defatted grape seeds protects HepG2 cells against oxidative stress by inducing phase II enzymes via Nrf2 activation

  • Younghwa Kim
  • Youngmin Choi
  • Hyeonmi Ham
  • Heon-Sang Jeong
  • Junsoo LeeEmail author
Research Article


Nuclear factor erythroid 2-related factor 2 (Nrf2) is an important transcription factor that regulates antioxidant response element (ARE)-driven phase II detoxification enzymes. In this study, induction of phase II enzymes via Nrf2/ARE activation in the cytoprotective effect of crude polyphenol extract (CPE), oligomeric procyanidin fraction (OPF), and polymeric procyanidin fraction (PPF) from defatted grape seeds in HepG2 cells was evaluated. Among these treatments, the treatment with PPF significantly increased Nrf2 protein expression in the nuclear fraction. Treating the samples increased heme oxygenase-1 (HO-1) and NAD(P)H:quinone oxidoreductase 1 (NQO1) protein expression in a dose-dependent manner, and PPF significantly increased the levels of phase II enzymes. Cellular generation of reactive oxygen species (ROS) were effectively reduced by PPF. These results suggest that pretreatment with PPF shows a cytoprotective effect by inhibiting ROS production and inducing HO-1 and NQO1 expression via Nrf2 activation in HepG2 cells.


procyanidin heme oxygenase-1 NAD(P)H: quinone oxidoreductase 1 nuclear factor-erythroid 2-related factor antioxidant response element 


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Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Younghwa Kim
    • 1
  • Youngmin Choi
    • 1
  • Hyeonmi Ham
    • 1
  • Heon-Sang Jeong
    • 1
  • Junsoo Lee
    • 1
    Email author
  1. 1.Department of Food Science and TechnologyChungbuk National UniversityCheongju, ChungbukKorea

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