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Isolation and characterization of an antimicrobial substance from Bacillus subtilis BY08 antagonistic to Bacillus cereus and Listeria monocytogenes

Food Science and Biotechnology volume 22, pages 433–440 (2013)Cite this article

Abstract

A wild-type bacteria producing an antimicrobial substance was isolated from Korean traditional fermented soybean paste, and identified as Bacillus subtilis. The antimicrobial substance purified by TLC, tentatively named UV254-B, displayed a specific antimicrobial activity against pathogenic Bacillus cereus and Listeria monocytogenes, without inhibiting the growth of soybean-fermenting Bacillus species. The antimicrobial substance was susceptible to proteinase K and lipase but resistant to esterase. Antimicrobial activity was observed over a wide range of pH from 3 to 11, with the maximum activity at pH 9, and thermal stability up to 80°C. The minimum inhibitory concentration of the antimicrobial substance was found to be 64 μg/mL for B. cereus and 128 μg/mL for L. monocytogenes. This antimicrobial substance has a putative molecular weight either at 1,133.6 or 1,700.5, which differs from that of other antimicrobial substances described for B. subtilis such as iturin, surfactin, fengycin, and subtilisin.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Chonbuk National University, Iksan, Jeonbuk, 570-752, Korea

    Wei-Jie Wu

  2. Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk, 570-752, Korea

    Seung-Moon Park

  3. Department of Oriental Medicine Resources, Chonbuk National University, Iksan, Jeonbuk, 570-752, Korea

    Byung-Yong Ahn

Authors
  1. Wei-Jie Wu
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  2. Seung-Moon Park
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Correspondence to Byung-Yong Ahn.

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Wu, WJ., Park, SM. & Ahn, BY. Isolation and characterization of an antimicrobial substance from Bacillus subtilis BY08 antagonistic to Bacillus cereus and Listeria monocytogenes . Food Sci Biotechnol 22, 433–440 (2013). https://doi.org/10.1007/s10068-013-0098-5

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  • DOI: https://doi.org/10.1007/s10068-013-0098-5

Keywords

  • antimicrobial
  • Bacillus cereus
  • LC/ESI-MS
  • Listeria monocytogenes
  • lipopeptide