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Anti-oxidant and anti-inflammatory properties of methanol extracts from various crops

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Abstract

Corn, sorghum, barley, Italian ryegrass (IRG), and alfalfa have long been used in human and/or animal diets. While their nutritional information is widely available, their potentials for anti-oxidation, anti-inflammation, and anti-tumor are barely explored. In the present study, several serial extracts were prepared from 70% methanol extracts of these forage crops and their bioactive potentials were investigated using cell-free and cell-mediated experimental systems. Chloroform extracts in this study generally showed stronger activity to scavenge hydroxyl radicals and superoxide anions than butanol or water extracts. IRG and sorghum chloroform extracts especially almost completely suppressed the lipopolysaccharide-mediated production of nitric oxide, tumor-necrosis factor-α, and interleukin-6. These extracts also inhibited dramatically the viability and DNA synthesis of human skin dermatofibrosarcoma cells without a toxic effect on primary cultured control cells. These beneficial effects appeared to be associated with the amount of flavonoids contained in the extracts.

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References

  1. Mates JM, Sanchez-Jimenez FM. Role of reactive oxygen species in apoptosis: Implications for cancer therapy. Int. J. Biochem. Cell Biol. 32: 157–170 (2000)

    Article  CAS  Google Scholar 

  2. Gandhi S, Abramov AY. Mechanism of oxidative stress in neurodegeneration. Oxid. Med. Cell Longev. 2012: 428010 (2012)

    Article  Google Scholar 

  3. Lee JC, Son YO, Pratheeshkumar P, Shi X. Oxidative stress and metal carcinogenesis. Free Radical Bio. Med. 53: 742–757 (2012)

    Article  CAS  Google Scholar 

  4. Rice-Evans CA, Miller NJ, Bolwell PG, Bramley PM, Pridham JB. The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radical Res. 22: 375–383 (1995)

    Article  CAS  Google Scholar 

  5. van Poppel G, van den Berg H. Vitamins and cancer. Cancer Lett. 114: 195–202 (1997)

    Article  Google Scholar 

  6. Jomova K, Valko M. Advances in metal-induced oxidative stress and human disease. Toxicology 283: 65–87 (2011)

    Article  CAS  Google Scholar 

  7. Firuzi O, Miri R, Tavakkoli M, Saso L. Antioxidant therapy: Current status and future prospects. Curr. Med. Chem. 18: 3871–3888 (2011)

    Article  CAS  Google Scholar 

  8. Awika JM, Rooney LW. Sorghum phytochemicals and their potential impact on human health. Phytochemistry 65: 1199–1221 (2004)

    Article  CAS  Google Scholar 

  9. Cuevas Montilla E, Hillebrand S, Antezana A, Winterhalter P. Soluble and bound phenolic compounds in different Bolivian purple corn (Zea mays L.) cultivars. J. Agr. Food Chem. 59: 7068–7074 (2011)

    Article  Google Scholar 

  10. Zilić S, Serpen A, Akıllıoğlu G, Gökmen V, Vančetović J. Phenolic compounds, carotenoids, anthocyanins, and antioxidant capacity of colored maize (Zea mays L.) kernels. J. Agr. Food Chem. 60: 1224–1231 (2012)

    Article  Google Scholar 

  11. Quinde-Axtell Z, Baik BK. Phenolic compounds of barley grain and their implication in food product discoloration. J. Agr. Food Chem. 54: 9978–9984 (2006)

    Article  CAS  Google Scholar 

  12. Gray AM, Flatt PR. Pancreatic and extra-pancreatic effects of the traditional anti-diabetic plant, Medicago sativa (lucerne). Brit. J. Nutr. 78: 325–334 (1997)

    Article  CAS  Google Scholar 

  13. Bora KS, Sharma A. Phytochemical and pharmacological potential of Medicago sativa: A review. Pharm. Biol. 49: 211–220 (2011)

    Article  Google Scholar 

  14. Halliwell B, Gutteridge JMC, Aruoma OI. The deoxyribose method: Simple “test-tube” assay for determination of rate constants for reactions of hydroxyl radicals. Anal. Biochem. 165: 215–219 (1987)

    Article  CAS  Google Scholar 

  15. Gotoh N, Niki E. Rates of interactions of superoxide with vitamin E, vitamin C, and related compounds as measured by chemiluminescence. Biochim. Biophys. Acta 1115: 201–207 (1992)

    Article  CAS  Google Scholar 

  16. Baek JA, Son YO, Fang M, Lee YJ, Cho HK, Whang WK, Lee JC. Catechin-7-O-β-d-glucopyranoside scavenges free radicals and protects human B lymphoma BJAB cells on H2O2-mediated oxidative stress. Food Sci. Biotechnol. 20: 151–158 (2011)

    Article  CAS  Google Scholar 

  17. Kook SH, Son YO, Choe Y, Kim JH, Jeon YM, Heo JS, Kim JG, Lee JC. Mechanical force augments the anti-osteoclastogenic potential of human gingival fibroblasts in vitro. J. Periodontal Res. 44: 402–410 (2009)

    Article  CAS  Google Scholar 

  18. Kujala TS, Loponen JM, Klika KD, Pihlaja K. Phenolics and betacyanins in red beetroot (Beta vulgaris) root: Distribution and effect of cold storage on the content of total phenolics and three individual compounds. J. Agr. Food Chem. 48: 5338–5342 (2000)

    Article  CAS  Google Scholar 

  19. Qawasmeh A, Obied HK, Raman A, Wheatley W. Influence of fungal endophyte infection on phenolic content and antioxidant activity in grasses: Interaction between Lolium perenne and different strains of Neotyphodium lolii. J. Agr. Food Chem. 60: 3381–3388 (2012)

    Article  CAS  Google Scholar 

  20. Hu C, Kitts DD. Studies on the antioxidant activity of Echinacea root extract. J. Agr. Food Chem. 48: 1466–1472 (2000)

    Article  CAS  Google Scholar 

  21. von Harsdorf R, Li PF, Dietz R. Signaling pathway in reactive oxygen species-induced cardiomyocyte apoptosis. Circulation 99: 2934–2941 (1999)

    Article  Google Scholar 

  22. Lee JC, Lim KT, Jang YS. Identification of Rhus verniciflua Stokes compounds that exhibit free radical scavenging and anti-apoptotic properties. Biochim. Biophys. Acta 1570: 181–191 (2002)

    Article  CAS  Google Scholar 

  23. Chan PC, Xia Q, Fu PP. Ginkgo biloba leave extract: Biological, medicinal, and toxicological effects. J. Environ. Sci. Heal. C 25: 211–244 (2007)

    CAS  Google Scholar 

  24. Ferretti G, Bacchetti T, Belleggia A, Neri D. Cherry antioxidants: From farm to table. Molecules 15: 6993–7005 (2010)

    Article  CAS  Google Scholar 

  25. Izzi V, Masuelli L, Tresoldi I, Sacchetti P, Modesti A, Galvano F, Bei R. The effects of dietary flavonoids on the regulation of redox inflammatory networks. Front. Biosci. 17: 2396–2418 (2012)

    Article  CAS  Google Scholar 

  26. Yu JY, Kim JH, Kim TG, Kim BT, Jang YS, Lee JC. (E)-1-(3,4-Dihydroxyphenethyl)-3-styrylurea inhibits proliferation of MCF-7 cells through G1 cell cycle arrest and mitochondria-mediated apoptosis. Mol. Cell 30: 303–310 (2010)

    Article  CAS  Google Scholar 

  27. Choi KC, Chung WT, Kwon JK, Jang YS, Yu JY, Park SM, Lee JC. Chemoprevention of a flavonoid fraction from Rhus verniciflua Stokes on aflatoxin B1-induced hepatic damage in mice. J. Appl. Toxicol. 31: 150–156 (2011)

    CAS  Google Scholar 

  28. Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Bio. Med. 20: 933–956 (1996)

    Article  CAS  Google Scholar 

  29. Kim SS, Son YO, Chun JC, Kim SE, Chung GH, Hwang KJ, Lee JC. Antioxidant property of an active component purified from the leaves of paraquat-tolerant Rehmannia glutinosa. Redox Rep. 10: 311–318 (2005)

    Article  CAS  Google Scholar 

  30. Stochmal A, Piacente S, Pizza C, De Riccardis F, Leitz R, Oleszek W. Alfalfa (Medicago sativa L.) flavonoids. 1. Apigenin and luteolin glycosides from aerial parts. J. Agr. Food Chem. 49: 753–758 (2001)

    Article  CAS  Google Scholar 

  31. Shukla S, Gupta S. Apigenin: A promising molecule for cancer prevention. Pharm. Res. 27: 962–978 (2010)

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Research Center of Bioactive Materials, Chonbuk National University, Jeonju, Jeonbuk, 561-756, Korea

    Jung-Min Hwang, Sung-Jun Bang, Beom-Tae Kim & Jeong-Chae Lee

  2. Institute of Oral Biosciences and BK21 Program, Chonbuk National University, Jeonju, Jeonbuk, 561-756, Korea

    Jung-Min Hwang & Jeong-Chae Lee

  3. Grassland and Forages Research Center, National Institute of Animal Science, Cheonan, Chungnam, 331-808, Korea

    Ki-Choon Choi, Gi Jun Choi & Da Hye Kim

  4. Graduate Center for Toxicology, College of Medicine, University of Kentucky, Lexington, KY, 40506, USA

    Young-Ok Son, Dong-Hern Kim & Xianglin Shi

Authors
  1. Jung-Min Hwang
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  2. Ki-Choon Choi
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  3. Sung-Jun Bang
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  4. Young-Ok Son
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  5. Beom-Tae Kim
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  6. Dong-Hern Kim
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  7. Gi Jun Choi
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  8. Da Hye Kim
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  9. Xianglin Shi
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  10. Jeong-Chae Lee
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Correspondence to Jeong-Chae Lee.

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Hwang, JM., Choi, KC., Bang, SJ. et al. Anti-oxidant and anti-inflammatory properties of methanol extracts from various crops. Food Sci Biotechnol 22 (Suppl 1), 265–272 (2013). https://doi.org/10.1007/s10068-013-0076-y

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  • DOI: https://doi.org/10.1007/s10068-013-0076-y

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