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Monitoring of radical scavenging compounds from oxidized lipids (RSOL) by 2,4-dinitrophenylhydrazine (DNPH) derivatization in thermally oxidized linoleic acid and lard systems

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Abstract

Oxidized lipids can decrease the absorbance of DPPH in isooctane. Changes of aldehydes, which can react with DPPH radicals, in oxidized linoleic acid and lard were monitored using 2,4-dinitrophenylhydrazine (DNPH) derivatization and HPLC. As thermal oxidation time increased in linoleic acid, peaks of propanal, pentanal, and hexanal increased proportionally. The correlation coefficient (r) of among DPPH absorbance and peak areas of DNPH derivatized lard for 360 min was −0.941. Peaks of t-2-heptenal and pentanal showed relatively high correlation with the changes of DPPH absorbance during lard oxidation. Quantity of oxidized products with radical scavenging ability in lipids can be determined by DNPH derivatization with HPLC analysis.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon, Gyeonggi, 440-746, Korea

    Eun Yeong Jang & JaeHwan Lee

  2. Department of Food Science and Technology, Seoul National University of Science and Technology, Seoul, 139-743, Korea

    Min Kyu Jeong & JuDong Yeo

Authors
  1. Min Kyu Jeong
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  2. JuDong Yeo
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  3. Eun Yeong Jang
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  4. JaeHwan Lee
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Correspondence to JaeHwan Lee.

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Jeong, M.K., Yeo, J., Jang, E.Y. et al. Monitoring of radical scavenging compounds from oxidized lipids (RSOL) by 2,4-dinitrophenylhydrazine (DNPH) derivatization in thermally oxidized linoleic acid and lard systems. Food Sci Biotechnol 22 (Suppl 1), 161–165 (2013). https://doi.org/10.1007/s10068-013-0062-4

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  • DOI: https://doi.org/10.1007/s10068-013-0062-4

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