Distribution of lactic acid bacteria in garlic (Allium sativum) and green onion (Allium fistulosum) using SDS-PAGE whole cell protein pattern comparison and 16S rRNA gene sequence analysis

Abstract

Distributions of lactic acid bacteria (LAB) in garlic and green onion samples as kimchi sub-ingredients were analyzed by comparing the SDS-PAGE whole cell protein patterns and 16S rRNA gene sequence analysis. In total, 245 LAB were isolated from 10 garlic samples and differentiated into 7 groups by comparing SDS-PAGE whole cell protein patterns. The groups were identified as Leuconostoc, Weissella, and Lactobacillus through the 16S rRNA gene sequence analysis. A total of 115 LAB were isolated from 7 green onion samples, differentiated into 6 groups, and identified as Weissella, Leuconostoc, and Lactococcus. Leuconostoc was the most dominated LAB in garlic and Weissella was the most dominated LAB in green onion. The LAB identified in this study was found as dominant microorganisms in kimchi. This result suggests the possible contribution of LAB in garlic and green onion to the bacterial microflora of kimchi, especially during early stage of fermentation.

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References

  1. 1.

    Lee SH, Kim SD. Effect of starter on the fermentation of kimchi. J. Korean Soc. Food Nutr. 17: 342–347 (1988)

    Google Scholar 

  2. 2.

    Lee JY, Choi MK, Kyung KH. Reappraisal of stimulatory effect of garlic on kimchi fermentation. Korean J. Food Sci. Technol. 40: 479–484 (2008)

    Google Scholar 

  3. 3.

    Cho NC, Jhon DY, Shin MS, Hong YH, Lim HS. Effect of garlic concentrations on growth of microorganisms during kimchi fermentation. Korean J. Food Sci. Technol. 20: 231–235 (1988)

    Google Scholar 

  4. 4.

    Rees LP, Minney SF, Plummer NT, Slater JH, Skyrme DA. A quantitative assessment of the antimicrobial activity of garlic (Allium sativum). World J. Microbiol. Biot. 9: 303–307 (1993)

    Article  Google Scholar 

  5. 5.

    Kyung KH, Park KS, Kim YS. Isolation and characterization of bacteria resistant to the antimicrobial activity of garlic. J. Food Sci. 61: 226–229 (1996)

    Article  CAS  Google Scholar 

  6. 6.

    Shim ST, Kyung KH. Natural microflora of prepeeled garlic and their resistance to garlic antimicrobial activity. Food Microbiol. 16: 165–172 (1999)

    Article  CAS  Google Scholar 

  7. 7.

    No HK, Lee SH, Kim SD. Effects of ingredients on fermentation of Chinese cabbage kimchi. Korean J. Soc. Food Nutr. 24: 642–650 (1995)

    Google Scholar 

  8. 8.

    Yi JH, Cho Y, Hwang IK. Effects of kimchi minor ingredients on the growth of lactic acid bacteria. Korean J. Soc. Food Sci. 11: 511–520 (1995)

    Google Scholar 

  9. 9.

    Mheen TI, Kwon TW. Effect of temperature and salt concentration on kimchi fermentation. Korean J. Food Sci. Technol. 16: 443–449 (1984)

    CAS  Google Scholar 

  10. 10.

    Shin DH, Kim MS, Han JS, Lim DK, Bak WS. Changes of chemical composition and microflora in commercial kimchi. Korean J. Food Sci. Technol. 28: 137–145 (1996)

    Google Scholar 

  11. 11.

    Lee CW, Ko CY, Ha DM. Microfloral changes of the lactic acid bacteria during kimchi fermentation and identification of the isolates. Korean J. Microbiol. Biotechnol. 20: 102–109 (1992)

    CAS  Google Scholar 

  12. 12.

    Kim JD, Chun JS, Han HU. Leuconostoc kimchii sp. nov., a new species from kimchi. Int. J. Syst. Evol. Micr. 50: 1915–1919 (2000)

    CAS  Google Scholar 

  13. 13.

    Lee JS, Lee KC, Ahn JS, Mheen TI, Pyun YR, Park YH. Weissella koreensis sp. nov., isolated from kimchi. Int. J. Syst. Evol. Micr. 52: 1257–1261 (2002)

    Article  CAS  Google Scholar 

  14. 14.

    Shim ST, Kyung KH, Yang YJ. Lactic acid bacteria isolated from fermenting kimchi and their fermentation of Chinese cabbage juice. Korean J. Food Sci. Technol. 22: 373–379 (1990)

    Google Scholar 

  15. 15.

    Cho JH, Lee DY, Yang CN, Jeon JI, Kim JH, Han HU. Microbial population dynamics of kimchi, a fermented cabbage product. FEMS Microbiol. Lett. 257: 262–267 (2006)

    Article  CAS  Google Scholar 

  16. 16.

    Lee MK, Park WS, Lee BH. Genetic identification of the kimchi strain using PCR-based PepN and 16S rRNA gene sequence. Korean J. Food Sci. Technol. 32: 1331–1335 (2000)

    Google Scholar 

  17. 17.

    Kim TW, Lee JY, Jung SH, Kim YM, Jo JS, Chung DK, Lee HJ, Kim HY. Identification and distribution of predominant lactic acid bacteria in kimchi, a Korean traditional fermented food. J. Microbiol. Biotechn. 12: 635–642 (2002)

    CAS  Google Scholar 

  18. 18.

    Lee JH, Choi JY, Kim MK. Reevaluation of the change of Leuconostoc species and Lactobacillus plantarum by PCR during Kimchi fermentation. J. Microbiol. Biotechn. 12: 166–171 (2002)

    Google Scholar 

  19. 19.

    Lee JS, Heo GY, Lee JW, Oh YJ, Park JA, Park YH. Analysis of kimchi microflora using denaturing gradient gel electrophoresis. Int. J. Food Microbiol. 102: 143–150 (2005)

    Article  CAS  Google Scholar 

  20. 20.

    Elliott JA, Collins MD, Pigott NE, Facklam RR. Differentiation of Lactococcus lactis and Lactococcus garvieae from humans by comparison of whole-cell protein patterns. J. Clin. Microbiol. 29: 2731–2734 (1991)

    CAS  Google Scholar 

  21. 21.

    Villani F, Moschetti G, Blaiotta G, Coppola S. Characterization of strains of Leuconostoc mesenteroides by analysis of soluble wholecell protein pattern, DNA fingerprinting, and restriction of ribosomal DNA. J. Appl. Microbiol. 82: 578–588 (1997)

    CAS  Google Scholar 

  22. 22.

    Kim, MJ, Chun JS. Bacterial community structure in kimchi, a Korean fermented vegetable food, as revealed by 16S rRNA gene analysis. Int. J. Food Microbiol. 103: 91–96 (2005)

    Article  CAS  Google Scholar 

  23. 23.

    National Institute of Health. National Center for Biotechnology Information. Available from: http://blast.ncbi.nlm.nih.gov. Accessed Mar. 27, 2012.

  24. 24.

    Ko YT, Lee SH. Quality characteristics of kimchi added with green tea powder. J. Korean Soc. Appl. Biol. Chem. 50: 281–286 (2007)

    Google Scholar 

  25. 25.

    Mheen TI. Kimchi. pp. 454–480. In: Microorganisms and Industry. Mheen TI (ed). Hanrimwon Pub., Co., Seoul, Korea (1998)

    Google Scholar 

  26. 26.

    Collins MD, Samelis J, Metaxopoulos J, Wallbanks S. Taxonomic studies on some Leuconostoc-like organisms from fermented sausages: Description of a new genus Weissella for the Leuconostoc paramesenteroides group of species. J. Appl. Bacteriol. 75: 595–603 (1993)

    Article  CAS  Google Scholar 

  27. 27.

    Bjorkroth KJ, Schillinger U, Geisen R, Weiss N, Hoste B, Holzapfel WH, Korkeala HJ, Vandamme P. Taxonomic study of Weissella confusa and description of Weissella cibaria sp. nov., detected in food and clinical samples. Int. J. Syst. Evol. Micr. 52: 141–148 (2002)

    CAS  Google Scholar 

  28. 28.

    Choi HJ, Cheigh CI, Kim SB, Lee JC, Lee DW, Choi SW, Park JM, Pyun YR. Weissella kimchii sp. nov., a novel lactic acid bacterium from kimchi. Int. J. Syst. Evol. Micr. 52: 507–511 (2002)

    CAS  Google Scholar 

  29. 29.

    Jang IC, Kim BJ, Lee JH, Kim JH, Jeong GJ, Han HU. Identification of Weissella species by the genus-specific amplified ribosomal DNA restriction analysis. FEMS Microbiol. Lett. 212: 29–34 (2002)

    Article  CAS  Google Scholar 

  30. 30.

    Kim TW, Lee JY, Song HS, Park JH, Ji GE, Kim HY. Isolation and identification of Weissella kimchii from green onion by cell protein pattern analysis. J. Microbiol. Biotechn. 14: 105–109 (2004)

    CAS  Google Scholar 

  31. 31.

    Kim H, Shim ST, Kim YS, Kyung KH. Diversity of Leuconostoc on garlic surface, and extreme environment. J. Microbiol. Biotechn. 12: 497–502 (2002)

    CAS  Google Scholar 

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Correspondence to Hae-Yeong Kim.

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Jung, HJ., Hong, Y., Yang, HS. et al. Distribution of lactic acid bacteria in garlic (Allium sativum) and green onion (Allium fistulosum) using SDS-PAGE whole cell protein pattern comparison and 16S rRNA gene sequence analysis. Food Sci Biotechnol 21, 1457–1462 (2012). https://doi.org/10.1007/s10068-012-0192-0

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Keywords

  • garlic
  • green onion
  • lactic acid bacteria
  • SDS-PAGE
  • 16S rRNA