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Potential use of natural pigments on laccase-based TTI Prototype: Substrate specificities and variations in Arrhenius activation energy

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Abstract

Non-toxic dyes and natural pigments were examined as potential substrates for a laccase-based time temperature integrator (TTI) prototype. Five compounds such as bromothymol blue (D1), methyl orange (D2), laccaic acid (P1), a pigment from bokbunja (Rubus coreanus) fruit (P2), and Gardenia blue pigment (P3) were all catalyzed, resulting in significant changes in color. CIE-Lab and ΔE appeared to have the most linear dependency on reaction time. Reaction rate constants, k, were estimated at 10–37°C with the coefficients of determination, R2, being the lowest at 0.94. The Arrhenius activation energies, Ea, were 42.6 66.1 kJ/mol depending on the substrate. The results show that alterations in Ea of this TTI is a very important finding in that Ea of the laccase-based TTI could be modulated with substrates to assess time-temperature history accurately and that a wide range of color compounds offer an opportunity for color-coded TTI to assure color blind individuals food safety.

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Authors and Affiliations

  1. Department of Food Science and Technology, Dongguk University-Seoul, Seoul, 100-715, Korea

    Eunji Kim, Yeon Ah Kim & Seung Ju Lee

  2. Department of Culinary Nutrition, Woosong University, Daejeon, 300-718, Korea

    Keehyuk Kim

Authors
  1. Eunji Kim
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  2. Keehyuk Kim
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  3. Yeon Ah Kim
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  4. Seung Ju Lee
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Correspondence to Seung Ju Lee.

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Kim, E., Kim, K., Kim, Y.A. et al. Potential use of natural pigments on laccase-based TTI Prototype: Substrate specificities and variations in Arrhenius activation energy. Food Sci Biotechnol 21, 1451–1456 (2012). https://doi.org/10.1007/s10068-012-0191-1

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  • DOI: https://doi.org/10.1007/s10068-012-0191-1

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