Prebiotic effect of mucilage and pectic-derived oligosaccharides from nopal (Opuntia ficus-indica)

Abstract

Prebiotic effect of mucilage (MO) and pectic-derived (PO) oligosaccharides from prickly pear cactus stems was assessed on both cultures and metabolic activity of microbial communities from the human colon. The MO treatment enhanced lactobacilli growth up to 23.8%, while PO increased the bifidobacteria population by 25%. Furthermore, the addition of MO produced a slight decrease in enterococci, enterobacteria, staphylococci, and clostridia of about 4%. Increased levels of the short-chain fatty acids (SCFA) were attained in the cultures at rates of 35 and 16% in response to MO and PO treatments, respectively. Propionic acid (propionate) and butanoic acid (butyrate) production increased at least 50% throughout MO and PO treatments. A decrease in the ammonium level of 11.5% was produced by MO treatment. This research indicates that a mixture of MO and PO oligosaccharides from nopal (Opuntia ficus-indica) could act as prebiotic.

This is a preview of subscription content, log in to check access.

References

  1. 1.

    Stintzing FC, Reinhold C. Cactus stems (Opuntia spp.): A review on their chemistry, technology, and uses. Mol. Nutr. Food Res. 49: 175–194 (2005)

    Article  CAS  Google Scholar 

  2. 2.

    Dongowsky G, Lorenz A. Unsaturated oligogalacturonic acids are generated by in vitro treatment of pectin with human fecal flora. Carbohyd. Res. 314: 237–244 (1998)

    Article  Google Scholar 

  3. 3.

    Olano-Martin E, Mountzouris KC, Gibson GR, Rastall RA. In vitro fermentability of dextran, oligodextran, and maltodextrin by human gut bacteria. Brit. J. Nutr. 83: 247–255 (2000)

    CAS  Google Scholar 

  4. 4.

    Rycroft CE, Jones MR, Gibson GR, Rastall RA. A comparative in vitro evaluation of the fermentation properties of prebiotic oligosaccharides. J. Appl. Microbiol. 91: 878–887 (2001)

    Article  CAS  Google Scholar 

  5. 5.

    Goycoolea F, Cardenas A. Pectins from Opuntia spp.: A short review. J. Prof. Assoc. Cactus 5: 17–23 (2003)

    Google Scholar 

  6. 6.

    Matsuhiro B, Lillo L, Sáenz C, Urzúa C, Zárate O. Chemical characterization of the mucilage from fruits of Opuntia ficus indica. Carbohyd. Polym. 63: 263–267 (2006)

    Article  CAS  Google Scholar 

  7. 7.

    Majdoub H, Roudesli S, Picton L, Le Cerf D, Muller G, Grisel M. Prickly pear nopals pectin from Opuntia ficus indica physicochemical study in dilute and semi-dilute solutions. Carbohyd. Polym. 46: 69–79 (2001)

    Article  CAS  Google Scholar 

  8. 8.

    de Sahagún, fray Bernardino. Códice Florentino. Colección Platina de la Biblioteca Médica (Platina Collection of the Medical Library), Archivo General de la Nación, Mexico City, México. pp. 2l8–220 (1979)

  9. 9.

    Sepúlveda E, Sáenz C, Aliaga E, Aceituno C. Extraction and characterization of mucilage in Opuntia spp. J. Arid Environ. 68: 534–545 (2007)

    Article  Google Scholar 

  10. 10.

    Olano-Martin E, Mountzouris KC, Gibson GR, Rastall RA. Continuous production of pectic-oligosaccharides in an enzyme membrane reactor. J. Food Sci. 66: 966–971 (2001)

    Article  CAS  Google Scholar 

  11. 11.

    AOAC. Official Methods of Analysis of AOAC Intl. 18th ed. Method 920.03. Association of Official Analytical Communities, Gaithersburg, MD, USA (2007)

    Google Scholar 

  12. 12.

    Fujiwara S, Seto Y, Kimura A, Hashiba H. Establishment of orally administered Lactobacillus gasseri SBT2055SR in the gastrointestinal tract of humans and its influence on intestinal microflora and metabolism. J. Appl. Microbiol. 90: 343–352 (2001)

    Article  CAS  Google Scholar 

  13. 13.

    Macfarlane GT, Steed H, Macfarlane S. Bacterial metabolism and health-related effects of galacto-oligosaccharides and other prebiotic. J. Appl. Microbiol. 104: 305–344 (2008)

    CAS  Google Scholar 

  14. 14.

    Maciorkowska E, Ryszczuk E, Kaczmarski M. The role of probiotics and prebiotics in apoptosis of the gastrointestinal tract. Prz. Gastroenterol. 5: 88–93 (2010)

    Google Scholar 

  15. 15.

    Bischoff SC, Schluck E. Pro- and prebiotics: A daily sip of health? Ernahrungs-Umschau. 57: 452–453 (2010)

    Google Scholar 

  16. 16.

    Uehara M, Ohta A, Sakai K, Suzuki K, Watanabe S, Adlercreutz H. Dietary fructooligosaccharides modify intestinal bioavailability of a single dose of genistein and daidzein and affect their urinary excretion and kinetics in blood of rats. J. Nutr. 131: 787–795 (2001)

    CAS  Google Scholar 

  17. 17.

    Torres DMP, Goncalves MDF, Teixeira JA, Rodriguez LR. Galactoologosaccharides: Production, properties, applications, and significance as prebiotics. Compr. Rev. Food Sci. F. 9: 438–454 (2010)

    Article  CAS  Google Scholar 

  18. 18.

    Louis P, Scoot KP, Duncan SH, Flint HJ. Understanding the effects of diet on bacterial metabolism in the large intestine. J. Appl. Microbiol. 102: 1197–1208 (2007)

    Article  CAS  Google Scholar 

  19. 19.

    Belenguer A, Duncan SH, Calder AG, Holtrop G, Louis P, Lobley GE, Flint HJ. Two routes of metabolic cross-feeding between Bifidobacterium adolescentis and butyrate-producing anaerobes from the human gut. Appl. Environ. Microb. 72: 3593–3599 (2006)

    Article  CAS  Google Scholar 

  20. 20.

    Duncan SH, Louis P, Flint HJ. Lactate-utilizing bacteria, isolated from human feces that produce butyrate as a major fermentation product. Appl. Environ. Microb. 70: 5810–5817 (2004)

    Article  CAS  Google Scholar 

  21. 21.

    Ichikawa H, Sakata T. Stimulation of epithelial cell proliferation of isolated distal colon of rats by continuous colonic infusion of ammonia or short-chain fatty acids is non additive. J. Nutr. 128: 843–847 (1998)

    CAS  Google Scholar 

  22. 22.

    Tuohy KM, Hinton DJS, Davies SJ, Crabbe MJC, Gibson GR, Ames JM. Metabolism of Maillard reaction products by the human gut microbiota implications for health. Mol. Nutr. Food Res. 50: 847–857 (2006)

    Article  CAS  Google Scholar 

  23. 23.

    Wutzke KD, Lotz M, Zipprich C. The effect of pre- and probiotics on the colonic ammonia metabolism in humans as measured by lactosa-[N-15(2)]ureide. Eur. J. Clin. Nutr. 64: 1215–1221 (2010)

    Article  CAS  Google Scholar 

  24. 24.

    Kolida S, Gibson GR. Prebiotic capacity of inulin-type fructans. J. Nutr. 137: 2503S–2506S (2007)

    CAS  Google Scholar 

  25. 25.

    Zanoni S, Pompei A, Cordisco L, Amaretti A, Rossi M, Matteuzzi D. Growth kinetics on oligo- and polysaccharides and promising features of three antioxidative potential probiotic strains. J. Appl. Microbiol. 105: 1266–1276 (2008)

    Article  CAS  Google Scholar 

  26. 26.

    Beards E, Touhy K, Gibson G. Bacterial, SCFA and gas profiles of a range of food ingredients following in vitro fermentation by human colonic microbiota. Anaerobe 16: 420–425 (2010)

    Article  CAS  Google Scholar 

  27. 27.

    Licht TR, Hansen M, Poulsen M, Dragsted LO. Dietary carbohydrate source influences molecular fingerprints of the rat faecal microbiota. BMC Microbiol. 6: 10–15 (2006)

    Article  Google Scholar 

  28. 28.

    Roberfroid MV, van Loo JAE, Gibson GR. The bifidogenic nature of chicory inulin and its hydrolysis products. J. Nutr. 128: 11–19 (1998)

    CAS  Google Scholar 

  29. 29.

    Rao VA. The prebiotic properties of oligofructose at low intake levels. Nutr. Res. 21: 843–848 (2001)

    Article  CAS  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Juan Carlos Guevara-Arauza.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Guevara-Arauza, J.C., de Jesús Ornelas-Paz, J., Pimentel-González, D.J. et al. Prebiotic effect of mucilage and pectic-derived oligosaccharides from nopal (Opuntia ficus-indica). Food Sci Biotechnol 21, 997–1003 (2012). https://doi.org/10.1007/s10068-012-0130-1

Download citation

Keywords

  • Bifidobacterium
  • Lactobacillus
  • prebiotic
  • short fatty acid
  • soluble dietary fiber