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Expression of the sweet-tasting protein brazzein in Lactobacillus spp.

Food Science and Biotechnology volume 21, pages 895–898 (2012)Cite this article

Abstract

As sucrose has been implicated in healthrelated problems such as obesity and diabetes, awareness of a safe natural non-sugar sweetener has increased. Brazzein is the intensely sweet-tasting plant protein which consists of 54 amino acids. In this study, brazzein was expressed in Lactobacillus spp., probiotics, which are widely used in food industry. Three Lactobacillus spp. (Lb. paracasei KLB58, Lb. plantarum KLB213, and Lb. reuteri KCTC3594) were electrotransformed with pMSP3545::Braht which contains brazzein gene along with nisin-controlled expression (NICE) system. Brazzein expression in Lactobacillus spp. was confirmed by Western blot analysis. Overall, higher expression rate was obtained with nisin concentration of 25–40 ng/mL.

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References

  1. National Cancer Institute Fact Sheet. Artificial sweeteners and cancer: Questions and answers. Available from: http://www.cancer.gov/cancertopics/factsheet/Risk/artificial-sweeteners#ref1. Accessed Jan. 7, 2010.

  2. Soffritti M, Belpoggi F, Esposti DD, Lambertini L. Aspartame induces lymphomas and leukaemias in rats. Eur. J. Oncol. 10: 107–116 (2005)

    Google Scholar 

  3. Faus I. Recent developments in the characterization and biotechnological production of sweet-tasting proteins. Appl. Microbiol. Biot. 53: 145–151 (2000)

    Article  CAS  Google Scholar 

  4. Ming D, Hellekant G. Brazzein, a new high-potency thermostable sweet protein from Pentadiplandra brazzeana B. FEBS Lett. 355: 106–108 (1994)

    Article  CAS  Google Scholar 

  5. Assadi-Porter FM, Aceti DJ, Cheng H, Markley JL. Efficient production of recombinant brazzein, a small, heat-stable, sweettasting protein of plant origin. Arch. Biochem. Biophys. 376: 252–258 (2000)

    Article  CAS  Google Scholar 

  6. Berlec A, Jevnikar Z, Majhenic AC, Rogelj I, Strukelj B. Expression of the sweet-tasting plant protein brazzein in Escherichia coli and Lactococcus lactis: A path toward sweet lactic acid bacteria. Appl. Microbiol. Biot. 73: 158–165 (2006)

    Article  CAS  Google Scholar 

  7. Lamphear BJ, Barker DK, Brooks CA, Delaney DE, Lane JR, Beifuss K, Love R, Thompson K, Mayor J, Clough R, Harkey R, Poage M, Drees C, Horn ME, Streatfield SJ, Nikolov Z, Woodard SL, Hood EE, Jilka JM, Howard JA. Expression of the sweet protein brazzein in maize for production of a new commercial sweetener. Plant Biotechnol. 3: 103–114 (2005)

    Article  CAS  Google Scholar 

  8. de Ruyter PG, Kuipers OP, de Vos WM. Controlled gene expression systems for Lactococcus lactis with the food-grade inducer nisin. Appl. Environ. Microb. 62: 3662–3667 (1996)

    Google Scholar 

  9. Berlec A, Tompa G, Slapar N, Fonovic UP, Rogelj I, Strukelj B. Optimization of fermentation conditions for the expression of sweettasting protein brazzein in Lactococcus lactis. Lett. Appl. Microbiol. 46: 227–231 (2008)

    Article  CAS  Google Scholar 

  10. Berlec A, Strukelj B. Large increase in brazzein expression achieved by chaning the plasmid/strain combination of the NICE system in Lactococcus lactis. Lett. Appl. Microbiol. 48: 750–755 (2009)

    CAS  Google Scholar 

  11. Sanders TAB. Food production and food safety. Brit. Med. J. 318: 1689–1693 (1999)

    Article  CAS  Google Scholar 

  12. Kleerebezem M, Boekhorst J, van Kranenburg R, Molenaar D, Kuipers OP, Leer R, Tarchini R, Peters SA, Sandbrink HM, Fiers MW, Stiekema W, Lankhorst RM, Bron PA, Hoffer SM, Groot MN, Kerkhoven R, de Vries M, Ursing B, de Vos WM, Siezen RJ. Complete genome sequence of Lactobacillus plantarum WCFS1. P. Natl. Acad. Sci. 100: 1990–1995 (2003)

    Article  CAS  Google Scholar 

  13. Wei MQ, Rush CM, Norman JM, Hafner LM, Epping RJ, Timms P. An improved methods for the transformation of Lactobacillus strains using electroporation. J. Microbiol. Meth. 21: 97–109 (1995)

    Article  Google Scholar 

  14. Lee YW, Im SH, Xin CF, So JS. Determination of optimal electrotransformation conditions for various Lactobacillus spp. Korean J. Biotechnol. Bioeng. 24: 182–188 (2009)

    Google Scholar 

  15. Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor, New York, NY, USA (1989)

    Google Scholar 

  16. Pavan S, Hols P, Delcour J, Geoffroy M, Grangette MC, Kleerebezem M, Mercenier A. Adaptation of the nisin-controlled expression in Lactobacillus plantarum: A tool to study in vivo biological effects. Appl. Environ. Microb. 66: 4427–4432 (2000)

    Article  CAS  Google Scholar 

  17. Chang CE, Pavlova SI, Tao L, Kim EK, Kim SC, Yun HS, So JS. Molecular identification of vaginal Lactobacillus spp. isolated from Korean women. J. Microbiol. Biotechn. 12: 312–317 (2000)

    Google Scholar 

  18. Bryan EM, Bae T, Kleerebezem M, Dunny GM. Improved vectors for nisin-controlled-expression in Gram-positive bacteria. Plasmid 44: 183–190 (2000)

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biological Engineering, Inha University, Incheon, 402-751, Korea

    Yoo-Won Lee, Kwan-Young Kim, Sang-Hyun Han, Chang-Ho Kang & Jae-Seong So

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  1. Yoo-Won Lee
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  2. Kwan-Young Kim
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  3. Sang-Hyun Han
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  4. Chang-Ho Kang
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  5. Jae-Seong So
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Correspondence to Jae-Seong So.

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Lee, YW., Kim, KY., Han, SH. et al. Expression of the sweet-tasting protein brazzein in Lactobacillus spp.. Food Sci Biotechnol 21, 895–898 (2012). https://doi.org/10.1007/s10068-012-0116-z

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  • DOI: https://doi.org/10.1007/s10068-012-0116-z

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