Skip to main content
SpringerLink
Log in

Production of Coturnix quail immunoglobulins Y (IgYs) against Vibrio parahaemolyticus and Vibrio vulnificus

  • Research Article
  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

Abstract

Production of chicken immunoglobulins Y (IgYs) and their applications in prophylactic, therapeutic, detection of microbial contaminants and as a diagnostic tool has been widely studied with limited information from other avians. This study produced Coturnix quail (Coturnix coturnix japonica) egg yolk IgYs against Vibrio parahaemolyticus and Vibrio vulnificus. Formalin inactivated (FIVP, FIVV, mixed FI-VP/VV) and heat inactivated (HIVP, HIVV, mixed HI-VP/VV) Vibrio immunogens (109 CFU/mL) were intramuscularly immunized into quail through thigh muscles. Egg yolk IgY was purified by water dilution-ammonium sulfate precipitation method and the activity was determined by enzyme-linked immunosorbent assay (ELISA). Formalin inactivated immunogens induced high humoral immune response for both V. parahaemolyticus and V. vulnificus over heat inactivated immunogens. However, IgYs resulted from HIVP and FIVV immunogens, showed high specificity to V. parahaemolyticus and V. vulnificus respectively. Detection limits of the indirect ELISA using the produced IgYs were 105 CFU/mL for V. parahaemolyticus and 106 CFU/mL for V. vulnificus. The developed antibodies showed high binding affinity to their corresponding immunogens, very little cross reactivity to Staphylococcus aureus and not other bacteria strains (p<0.05), a phenomenon which was also observed in Western blot.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Lee JK, Jung DW, Eom SY, Oh SW, Kim Y, Kwak HS, Kim YH. Occurrence of Vibrio parahaemolyticus in oysters from Korean retail outlets. Food Control 19: 990–994 (2008)

    Article  Google Scholar 

  2. Michael A, Meenatchisundaram S, Parameswari G, Subbraj T, Selvakumaran R, Ramalingam S. Chicken egg yolk antibodies (IgY) as an alternative to mammalian antibodies. Indian J. Sci. Technol. 3: 468–474 (2010)

    CAS  Google Scholar 

  3. De Meulenaer B, Baert K, Lanckriet H, Van Hoed V, Huyghebaert A. Development of an enzyme-linked immunosorbent assay for bisphenol A using chicken immunoglobulins. J. Agr. Food Chem. 50: 5273–5282 (2002)

    Article  Google Scholar 

  4. Dias da Silva W, Tambourgi DV. IgY: A promising antibody for use in immunodiagnostic and in immunotherapy. Vet. Immunol. Immunop. 135: 173–180 (2010)

    Article  CAS  Google Scholar 

  5. Allen JC, Smith CJ. Enzyme-linked immunoassay kits for routine food analysis. Trends Biotechnol. 5: 193–199 (1987)

    Article  CAS  Google Scholar 

  6. Pauly D, Dorner M, Zhang X, Hlinak A, Dorner B, Schade R. Monitoring of laying capacity, immunoglobulin Y concentration, and antibody titer development in chickens immunized with ricin and botulinum toxins over a two-year period. Poultry Sci. 88: 281–290 (2009)

    Article  CAS  Google Scholar 

  7. Sunwoo HH, Wang WW, Sim JS. Detection of Escherichia coli O157:H7 using chicken immunoglobulin Y. Immunol. Lett. 106: 191–193 (2006)

    Article  CAS  Google Scholar 

  8. Chang HM, Ou-Yang RF, Chen YT, Chen CC. Productivity and some properties of immunoglobulin specific against Streptococcus mutans serotype c in chicken egg yolk (IgY). J. Agr. Food Chem. 47: 61–66 (1999)

    Article  CAS  Google Scholar 

  9. Hirai K, Arimitsu H, Umeda K, Yokota K, Shen L, Ayada K, Kodama Y, Tsuji T, Hirai Y, Oguma K. Passive oral immunization by egg yolk immunoglobulin (IgY) to Vibrio cholerae effectively prevents cholera. Acta Med. Okayama 64: 163–170 (2010)

    Google Scholar 

  10. Lee SB, Mine Y, Stevenson RMW. Effects of hen egg yolk immunoglobulin in passive protection of rainbow trout against Yersinia ruckeri. J. Agr. Food Chem. 48: 110–115 (2000)

    Article  CAS  Google Scholar 

  11. Lee EN, Sunwoo HH, Menninen K, Sim JS. In vitro studies of chicken egg yolk antibody (IgY) against Salmonella Enteritidis and Salmonella typhymurium. Poultry Sci. 81: 632–641 (2002)

    CAS  Google Scholar 

  12. Schade R, Hlinak A. Egg yolk antibodies, state of the art and future prospects. ALTEX 13(5): 5–9 (1996)

    Google Scholar 

  13. Akita EM, Nakai S. Immunoglobulins from egg yolk: Isolation and purification. J. Food Sci. 57: 629–634 (1992)

    Article  CAS  Google Scholar 

  14. Kovgan AA, Isaeva EI, Rovnova ZI, Shilov AA. Specific immunoglobulins from egg yolks of Japanese quail immunized with influenza and HIV-1 viruses. Dokl. Akad. Nauk SSSR 307: 229–233 (1989)

    CAS  Google Scholar 

  15. Somowiyarjo S, Sako N, Nonaka F. Production of avian antibodies to three potyviruses in coturnix quail. J. Virol. Methods 28: 125–132 (1990)

    Article  CAS  Google Scholar 

  16. Bae HD, Kitaguchi K, Horio F, Murai A. Higher incorporation of heterologous chicken immunoglobulin Y compared with homologous quail immunoglobulin Y into egg yolks of Japanese quail (Coturnix japonica). Poultry Sci. 88: 1703–1711 (2009)

    Article  CAS  Google Scholar 

  17. Ko KY, Ahn DU. Preparation of immunoglobulin Y from egg yolk using ammonium sulphate precipitation and ion exchange chromatography. Poultry Sci. 86: 400–407 (2007)

    CAS  Google Scholar 

  18. Deignan T, Kelly J, Alwan A, O’Farrelly C. Comparative analysis of methods of purification of egg yolk immunoglobulin. Food Agric. Immunol. 12: 77–85 (2000)

    Article  CAS  Google Scholar 

  19. Jensenius JC, Andersen I, Hau J, Crone M, Koch C. Eggs: Conveniently packaged antibodies. Methods for purification of yolk IgG. J. Immunol. Methods 46(1): 63–68 (1981)

    Article  CAS  Google Scholar 

  20. Polson A, Von Wechmar MB, Van Regenmortel MH. Isolation of viral IgY antibodies from yolks of immunized hens. Immunol. Commun. 9: 475–493 (1980)

    CAS  Google Scholar 

  21. Polson A, Coetzer T, Kruger J, Von Maltzahn E, Van der Merwe KJ. Improvements in the isolation of IgY from the yolks of eggs laid by immunized hens. Immunol. Invest. 14: 323–327 (1985)

    Article  CAS  Google Scholar 

  22. Abeyrathne PD, Lam JS. Conditions that allow for effective transfer of membrane protein onto nitrocellulose membrane in Western blot. Can. J. Microbiol. 53: 526–532 (2007)

    Article  CAS  Google Scholar 

  23. Shahhosseini S, Guttikonda S, Bhatnagar P, Suresh MR. Production and characterization of monoclonal antibodies against shope fibroma virus superoxide dismutase and glutathione-S-transferase. J. Pharm. Pharm. Sci. 9: 165–168 (2006)

    CAS  Google Scholar 

  24. Dong D, Liu H, Xiao Q, Li R. Affinity purification of egg yolk immunoglobulins (IgY) with a stable synthetic ligand. J. Chromatogr. B 870: 51–54 (2008)

    Article  CAS  Google Scholar 

  25. Javier HernáNdez-Campos F, Brito-De La Fuente E, Torrestiana-SáNchez B. Purification of egg yolk immunoglobulin (IgY) by ultrafiltration: Effect of pH, ionic strength, and membrane properties. J. Agr. Food Chem. 58: 187–193 (2010)

    Article  Google Scholar 

  26. Grey HM. Duck immunoglobulins. II. Biologic and immunochemical studies. Eur. J. Immunol. 98: 820–826 (1967)

    CAS  Google Scholar 

  27. Larsson A, Jonsson L, Sjöquist J. Determination of circulating immune complexes by chicken anti-human C3 and anti-human C1q micro ELISA. J. Immunol. Methods 113(1): 93–99 (1988)

    Article  CAS  Google Scholar 

  28. Chalghoumi R, Théwis A, Portetelle D, Beckers Y. Production of hen egg yolk immunoglobulins simultaneously directed against Salmonella Enteritidis and Salmonella Typhimurium in the same egg yolk. Poultry Sci. 87: 32–40 (2008)

    Article  CAS  Google Scholar 

  29. Kim M, Higashiguchi S, Iwamoto Y, Yang HC, Cho HY, Hatta H. Egg yolk antibody and its application. Biotechnol. Biopro. Eng. 5: 79–83 (2000)

    Article  CAS  Google Scholar 

  30. Mahdavi AH, Rahmani HR, Nili N, Samie AH, Soleimanian-Zad S. Chicken egg yolk antibody (IgY) powder against Escherichia coli O78:K80. J. Anim. Vet. Adv. 9: 366–373 (2010)

    Article  Google Scholar 

  31. Shin JH, Yang M, Nam SW, Kim JT, Myung NH, Bang WG, Roe IH. Use of egg yolk-derived immunoglobulin as an alternative to antibiotic treatment for control of Helicobacter pylori infection. Clin. Diagn. Lab. Immun. 9: 1061–1066 (2002)

    CAS  Google Scholar 

  32. Yegani M, Korver DR. Application of egg yolk antibodies as replacement for antibiotics in poultry. World. Poultry Sci. J. 66: 27–37 (2010)

    Article  Google Scholar 

  33. Kirkman TW. Statistics to Use. (1996) Available from: http://www.physics.csbsju.edu/stats/. Accessed Sept. 20, 2011.

Download references

Author information

Authors and Affiliations

  1. Division of Applied Life Science (BK 21 Program), Graduate School of Gyeongsang National University, Jinju, Gyeongnam, 660-701, Korea

    Neema Kassim, Adelard B. Mtenga, Won-Gyeong Lee & Duck-Hwa Chung

  2. Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Gyeongnam, 660-701, Korea

    Jeong-Sook Kim

  3. The College of Human Sciences, Nutrition, Food and Exercise Sciences, Florida State University, P. O. Box 3061493, Tellahassee, FL, 32306, USA

    Won-Bo Shim

Authors
  1. Neema Kassim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Adelard B. Mtenga
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Won-Gyeong Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jeong-Sook Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Won-Bo Shim
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Duck-Hwa Chung
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Duck-Hwa Chung.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kassim, N., Mtenga, A.B., Lee, WG. et al. Production of Coturnix quail immunoglobulins Y (IgYs) against Vibrio parahaemolyticus and Vibrio vulnificus . Food Sci Biotechnol 20, 1577–1583 (2011). https://doi.org/10.1007/s10068-011-0218-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10068-011-0218-z

Keywords

Search

Navigation