Skip to main content

Rapid concentration of some bacteriocin-like compounds using an organic solvent


A new solvent extraction method for isolation of bacteriocin-like compounds (BLCs) from bacterial culture broth was developed. Culture supernatant of Pediococcus acidilactici WRL-1 was mixed with 3 volumes of cold acetone and maintained at −20°C for 2 h. The solvent and precipitate were separated by centrifugation. Most (95.2 ±2.5%) pediocin activity was found in the liquid phase and it was obtained by evaporation. This method was also an effective means of isolating other BLCs from cultures of Lactobacillus acidophilus, Lactobacillus plantarum, and Bacillus licheniformis DJ-2. Other solvents (methanol, ethanol, and butanol) were also suitable toward this end.

This is a preview of subscription content, access via your institution.


  1. Klaenhammer TR. Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol. Rev. 12: 39–85 (1993)

    CAS  Google Scholar 

  2. Gonzalez B, Arca P, Mayo B, Saurez JE. Detection, purification, and partial characterization of plantaricin C, a bacteriocin produced by a Lactobacillus plantarum strain of dairy origin. Appl. Environ. Microb. 60: 2158–2163 (1994)

    CAS  Google Scholar 

  3. Dimick DP, Alderton G, Lewis JC, Lightbody HD, Fevold HL. Purification and some properties of subtilin. Arch. Biochem. 15: 1–11 (1947)

    CAS  Google Scholar 

  4. Cheeseman GC, Berridge NJ. An improved method of preparing nisin. Biochem. J. 65: 603–608 (1957)

    CAS  Google Scholar 

  5. Muriana PM, Klaenhammer TR. Purification and partial characterization of Lactacin F, a bacteriocin produced by Lactobacillus acidophilus 11088. Appl. Environ. Microb. 57: 114–121 (1991)

    CAS  Google Scholar 

  6. Yang R, Johnson MC, Ray B. Novel method to extract large amounts of bacteriocins from lactic acid bacteria. Appl. Environ. Microb. 58: 3355–3359 (1992)

    CAS  Google Scholar 

  7. Burianek LL, Yousef AE. Solvent extraction of bacteriocins from liquid cultures. Lett. Appl. Microbiol. 31: 193–197 (2000)

    Article  CAS  Google Scholar 

  8. Choi NS, Jeong SY, Yang HJ, Ahn KH, Park CS, Kim CY, Kim JS, Yoon BD, Kim MS. Activity assay for nisin-like acidic bacteriocins using an optimal pH-conditioned gel matrix. Anal. Biochem. 397: 259–261 (2010)

    Article  CAS  Google Scholar 

  9. Schagger H, von Jagow G. Tricine-sodium dodecyl sulfatepolyacrylamide gel electrophoresis for the separation of proteins in the range from 1–100 kDa. Anal. Biochem. 166: 368–379 (1987)

    Article  CAS  Google Scholar 

  10. Chung DM, Kim KE, Ahn KH, Park CS, Kim DH, Koh HB, Chun HK, Yoon BD, Kim HJ, Kim MS, Choi NS. Silver-stained fibrin zymography: Separation of proteases and activity detection using a single substrate-containing gel. Biotechnol. Lett. 33: 1663–1666 (2011)

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations


Corresponding author

Correspondence to Nack-Shick Choi.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Chung, DM., Kim, K.E., Jeong, SY. et al. Rapid concentration of some bacteriocin-like compounds using an organic solvent. Food Sci Biotechnol 20, 1457 (2011).

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • DOI:


  • bacteriocin-like compound
  • organic solvent
  • anti-microbial
  • Bacillus cereus