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Rapid concentration of some bacteriocin-like compounds using an organic solvent

Food Science and Biotechnology volume 20, Article number: 1457 (2011) Cite this article

Abstract

A new solvent extraction method for isolation of bacteriocin-like compounds (BLCs) from bacterial culture broth was developed. Culture supernatant of Pediococcus acidilactici WRL-1 was mixed with 3 volumes of cold acetone and maintained at −20°C for 2 h. The solvent and precipitate were separated by centrifugation. Most (95.2 ±2.5%) pediocin activity was found in the liquid phase and it was obtained by evaporation. This method was also an effective means of isolating other BLCs from cultures of Lactobacillus acidophilus, Lactobacillus plantarum, and Bacillus licheniformis DJ-2. Other solvents (methanol, ethanol, and butanol) were also suitable toward this end.

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Authors and Affiliations

  1. Woori Life Science Co., Ltd., Cheonan, Chungnam, 330-858, Korea

    Ki Eun Kim, Hong Jib Kim & Nack-Shick Choi

  2. Institute Bioindustry Research Center, KRIBB, Jeongeup, Jeonbuk, 580-185, Korea

    Dong-Min Chung, Seong-Yeop Jeong, Keug-Hyun Ahn, Hyo Kon Chun & Byung-Dae Yoon

  3. Department of Food Science & Technology, Catholic University of Daegu, Gyeongsan, Gyeongbuk, 712-702, Korea

    Chang-Su Park

  4. Advanced Radiation Technology Research Institute, KAERI, Jeonbuk Branch Institute, Jeongeup, Jeonbuk, 580-185, Korea

    Dong Ho Kim

  5. Department of Biochemistry and Health Science, Changwon National University, Changwon, Gyeongnam, 641-773, Korea

    Seong-Yeop Jeong & Dae-Ook Kang

  6. Colleage of Veterinary Medicine, Chonnam National University, Gwangju, 500-757, Korea

    Ki Eun Kim & Hong Bum Koh

Authors
  1. Dong-Min Chung
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  2. Ki Eun Kim
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  3. Seong-Yeop Jeong
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  4. Chang-Su Park
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  5. Keug-Hyun Ahn
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  6. Dong Ho Kim
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  7. Dae-Ook Kang
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  8. Hyo Kon Chun
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  9. Byung-Dae Yoon
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  10. Hong Bum Koh
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  11. Hong Jib Kim
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  12. Nack-Shick Choi
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Corresponding author

Correspondence to Nack-Shick Choi.

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Chung, DM., Kim, K.E., Jeong, SY. et al. Rapid concentration of some bacteriocin-like compounds using an organic solvent. Food Sci Biotechnol 20, 1457 (2011). https://doi.org/10.1007/s10068-011-0201-8

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  • DOI: https://doi.org/10.1007/s10068-011-0201-8

Keywords

  • bacteriocin-like compound
  • organic solvent
  • anti-microbial
  • Bacillus cereus