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Analysis of microflora in gochujang, Korean traditional fermented food

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Abstract

To identify and characterize the microbial communities in gochujang, a traditional Korean fermented food, the viable cell numbers of total bacteria, halophilic bacteria, fungi, yeasts, halophilic yeasts, and lactic acid bacteria from 7 homemade gochujang samples produced in the Sunchang area and 5 commercial gochujang samples were counted. The variety of microflora in the gochujang samples was evaluated by identifying the isolates via DNA sequence analysis. The viable cell numbers of total bacteria were determined to be in the range of 6–7 log CFU/g, which were higher than those of yeasts. Neither lactic acid bacteria nor fungi were detected in either type of gochujang after aging. A total of 7 genera and 31 species of microorganisms were identified in the gochujang: 29% was identified as Bacillus velezensis, as a major microorganism in gochujang. Under halophilic conditions, Oceanobacillus spp. was detected for the first time.

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References

  1. Shin DH. Survey on preparation method of traditional home made gochujang (fermented hot pepper-soybean paste). Korean J. Diet. Culture 10: 427–434 (1995)

    Google Scholar 

  2. Ko BK. Preparation and physicochemical properties of abalone gochujang. PhD thesis, Chonnam National Universuty, Gwangju, Korea (2006)

    Google Scholar 

  3. Choi JY, Lee TS, Noh BS. Quality characteristics of the gochujang prepared with mixture of meju and koji during fermentation. Korean J. Food Sci. Technol. 32: 125–131 (2000)

    Google Scholar 

  4. Cho DH, Lee WJ. Microbiological studies of Korean native soysauce fermentation: A study on the microflora of fermented Korean meju loaves. J. Korean Soc. Agric. Chem. 13: 35–42 (1970)

    Google Scholar 

  5. Lee SS. The fungi inhabiting Korean traditional meju (meju fungi). Soy Sauce Institute, Yeung Nam University, Daegu, Korea (2000)

    Google Scholar 

  6. Choi KK, Cui CC, Ham SS, Lee DS. Isolation, identification, and growth characteristics of main strain related to meju fermentation. J. Korean Soc. Food Sci. Nutr. 32: 818–824 (2003)

    Article  CAS  Google Scholar 

  7. Kim SR, Huh DJ. Studies on improvement of traditional condiment. Natl. Def. Sci. Ins. 56: 9277–9281 (1954)

    Google Scholar 

  8. Han YS, Park BD. Studies on the manufacturing of soy-sauce (part 1); Aspergillus oryzae from ordinary meju and gokja research report. Cen. Ind. Res. Ins. 7: 51–56 (1957)

    Google Scholar 

  9. Kang MJ, Kim SH, Joo HK, Lee GS, Yim MH. Isolation and identification of microorganisms producing the soy proteinhydrolyzing enzyme from traditional meju. J. Korean Soc. Agric. Chem. 43: 86–94 (2000)

    CAS  Google Scholar 

  10. Lee JS, Yi SH. Isolation, identification, and cultural conditions of yeast from traditional meju. Korean J. Appl. Microbiol. Biotechnol. 25: 435–441 (1997)

    Google Scholar 

  11. Shin DH. Functionality of soy fermented food and changes of manufacturing technology. Korea Soybean Digest 21: 49–63 (2004)

    Google Scholar 

  12. Yoon JH, Lee ST, Park YH. Inter-and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int. J. Syst. Bacteriol. 48: 187–194 (1996)

    Article  Google Scholar 

  13. Gürtler V, Stanisich VA. New approaches to typing and identification of bacteria using the 16S–23S rDNA spacer region. Microbiology 142: 3–16 (1996)

    Article  Google Scholar 

  14. White TJ, Bruns TD, Lee SB, Taylor JW. Amplification and direct sequencing of fungal ribosomal DNA for phylogenetics. pp. 315–322. In: PCR Protocols: A Guide to the Methods and Applications. Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds). Academic Press, Inc., New York, NY, USA (1990)

    Google Scholar 

  15. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties, and weight matrix choice. Nucleic Acids Res. 22: 4673–4680 (1994)

    Article  CAS  Google Scholar 

  16. Saitou N, Nei M. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406–425 (1987)

    CAS  Google Scholar 

  17. Kumar S, Tamura K, Jakobsen IB, Nei M. MEGA2: Molecular evolutionary genetics analysis software. Bioinformatics 17: 1244–1245 (2001)

    Article  CAS  Google Scholar 

  18. Shin DH, Kim DH, Choi U, Lim DK, Lim MS. Studies on the physicochemical characteristics of traditional gochujang. Korean J. Food Sci. Technol. 28: 157–161 (1996)

    Google Scholar 

  19. Lee JS, Choi YJ. Screening and characterization of osmotolerant and gas-producing yeasts from traditional deonjang and gochujang. Food Biotechnol. 5: 54–58 (1996)

    Google Scholar 

  20. Kim JS, Choi SH, Lee SD, Lee GH, Oh MJ. Quality change of sterilized soybean paste during its storage. J. Korean Soc. Food Sci. Nutr. 28: 1069–1075 (1999)

    Google Scholar 

  21. Kwon OJ, Kim JK, Chung YG. The characteristics of bacteria isolated from ordinary Korean soy-sauce and soybean paste. Agric. Chem. Biotechnol. 29: 422–428 (1986)

    Google Scholar 

  22. Jung YS. Microbiological studies of soy sauce: Identification and isolation of bacteria from traditional soy sauce. Korean J. Microbiol. 1: 30–35 (1963)

    Google Scholar 

  23. Yoo SK, Cho WH, Kang SM, Lee SH. Isolation and identification of microorganisms in Korean traditional soybean paste and soybean sauce. Korean J. Appl. Microbiol. Biotechnol. 27: 113–117 (1999)

    Google Scholar 

  24. Sneath PA, Mair NS. Bergey’s Manual of Systematic Bacteriology. Vol. 2. Williams & Wilkins, Baltimore, MD, USA. pp. 1105–1139 (1986)

    Google Scholar 

  25. Arbige NV, Bulthuis BA, Schultz J, Crabb D. Fermentation of Bacillus. pp. 871–895. In: Bacillus subtilis and Other Gram-positive Bacteria; Biochemistry, Physiology and Molecular Genetics. Sonenshein AL, Hoch JA, Losick, R (eds). Wiley-Blackwell, Hoboken, NJ, USA (1993)

    Google Scholar 

  26. Katz E, Demain AL. The peptide antibiotics of Bacillus: Chemistry, biogenesis, and possible function. Bacteriol. Rev. 41: 449–474 (1977)

    CAS  Google Scholar 

  27. Banwart GJ. Basic Food Mirobiology. 2nd ed. Chapman & Hall, New York, NY, USA. pp. 461–462 (1989)

    Google Scholar 

  28. Ruiz G. Bacillus velezensis sp. nov., a surfactant-producing bacterium isolated from the river Velez in Malaga, southern Spain. Int. J. Syst. Evol. Micr. 55: 191–195 (2005)

    Article  Google Scholar 

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Jang, SJ., Kim, YJ., Park, JM. et al. Analysis of microflora in gochujang, Korean traditional fermented food. Food Sci Biotechnol 20, 1435–1440 (2011). https://doi.org/10.1007/s10068-011-0197-0

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