Skip to main content
Log in

Evaluation of antioxidant and antimicrobial potential of strawberry tree (Arbutus Unedo L.) leaf

  • Research Article
  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

Abstract

In present study, antioxidant and antimicrobial activities of strawberry tree (Arbutus unedo L.) leaves were investigated. Antioxidant activity was determined by methods of DPPH scavenging, β-carotene bleaching, reducing power, metal chelating, superoxide anion scavenging, and hydrogen peroxide scavenging activity. Total phenolic content were determined to be 197.16±1.43 mg GAE/g extract in aqueous extract. The EC50 value of methanolic extracts was found to be 0.423 mg/mL. The extracts of leaves showed nearly 1/4 metal chelating capacity of standard EDTA, high reducing power, superoxide anion scavenging, and hydrogen peroxide scavenging activities. While the strawberry tree leaves exhibited antibacterial activity against Staphylococcus aureus, there was no inhibitory effect against Escherichia coli and Salmonella enteritidis. The strawberry tree leaves exhibited antifungal effect against 2 aflatoxigenic molds namely Aspergillus parasiticus NRRL 2999 and NRRL 465. These results suggest that the strawberry tree leaves may be used as an antioxidant source for pharmaceutical application, nutraceutical and functional food industries.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Ayaz FA, Kucukkislamoglu M, Reunanen M. Sugar, non-volatile and phenolic acids composition of strawberry tree (Arbutus unedo L. var. ellipsoidea) fruits. J. Food Compos. Anal. 13: 171–177 (2000)

    Article  CAS  Google Scholar 

  2. Tahraoui A, El-Hilali J, Israili ZH, Lyoussi B. Ethnopharmacological survey of plants used in the traditional treatment of hypertension and diabetes in the south-eastern Morocco (Errachidia province). J. Ethnopharmacol. 110: 105–117 (2007)

    Article  CAS  Google Scholar 

  3. Barros L, Carvalho AM, Sá Morais J, Ferreira ICFR. Strawberrytree, blackthorn, and rose fruits: Detailed characterisation in nutrients and phytochemicals with antioxidant properties. Food Chem. 120: 247–254 (2010)

    Article  CAS  Google Scholar 

  4. Celikel G, Demirsoy L, Demirsoy H. The strawberry tree (Arbutus unedo L.) selection in Turkey. Sci. Hortic. Amsterdam 118: 115–119 (2008)

    Article  Google Scholar 

  5. Percival M. Antioxidants. Clin. Nutr. Insights 10: 1–4 (1998)

    Google Scholar 

  6. Pabuccuoglu A, Kivcak B, Bas M, Mert T. Antioxidant activity of Arbutus unedo leaves. Fitoterapia 74: 597–599 (2003)

    Article  CAS  Google Scholar 

  7. Mariotto S, Esposito E, Di Paola R, Ciampa A, Mazzone E, Carcereri de Prati A, Darra E, Vincenzo S, Cucinotta G, Caminiti R, Suzuki H, Cuzzocrea S. Protective effect of Arbutus unedo aqueous extract in carrageenan-induced lung inflammation in mice. Pharmacol. Res. 57: 110–124 (2008)

    Article  Google Scholar 

  8. Oliveira I, Coelho V, Baltasar R, Pereira JA, Baptista P. Scavenging capacity of strawberry tree (Arbutus unedo L.) leaves on free radicals. Food Chem. Toxicol. 47: 1507–1511 (2009)

    Article  CAS  Google Scholar 

  9. Slinkard K, Singleton VL. Total phenol analyses: Automation and comparison with manual methods. Am. J. Enol. Viticult. 28: 49–55 (1977)

    CAS  Google Scholar 

  10. Miller NJ, Luiz-Larrea MB. Flavonoids and other plant phenols in the diet: Their significance as antioxidants. J. Nutr. Environ. Med. 12: 39–51 (2002)

    Article  CAS  Google Scholar 

  11. Blois MS. Antioxidant determinations by the use of stable free radical. Nature 26: 1199–1200 (1958)

    Article  Google Scholar 

  12. Al-Saikhan MS, Howard LR, Miller JC. Antioxidant activity and total phenolics in different genotypes of potato (Solanum tuberosum, L.). J. Food Sci. 60: 341–343 (1995)

    Article  CAS  Google Scholar 

  13. Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Jpn. J. Nutr. 44: 307–315 (1986)

    Article  CAS  Google Scholar 

  14. Dinis TCP, Madeia VMC, Almeida LM. Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) assay inhibitors of membrane lipid peroxidation and assay peroxyl radical scavengers. Arch. Biochem. Biophys. 315: 161–169 (1994)

    Article  CAS  Google Scholar 

  15. Nishikimi M, Rao NA, Yagi K. The occurance of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem. Bioph. Res. Co. 46: 849–854 (1972)

    Article  CAS  Google Scholar 

  16. Ruch RJ, Chung SU, Klaunig JE. Spin trapping of superoxide and hydroxyl radicals. Method. Enzymol. 105: 198–209 (1984)

    Article  Google Scholar 

  17. Morin P, Pouliot Y, Jiménez-Flores R. A comparative study of the fractionation of regular buttermilk and whey buttermilk by microfiltration. J. Food Eng. 77: 521–528 (2006)

    Article  CAS  Google Scholar 

  18. Janssen AM, Scheffer JJC, Baerheim SA. Antimicrobial activities of essential oils: A 1976–1986 literature review. Aspects of the test methods. Planta Med. 53: 395–398 (1987)

    Article  CAS  Google Scholar 

  19. Steel RGD, Torrie JH. Principles and Procedures of Statistics. McGraw Hill Book Co., Inc., New York, NY, USA. pp.107–109 (1960)

    Google Scholar 

  20. Yi O, Jovel EM, Towers NGH, Wahbe TR, Cho D. Antioxidant and antimicrobial activities of native Rosa sp. from British Columbia, Canada. Int. J. Food. Sci. Nutr. 58: 178–189 (2007)

    Article  Google Scholar 

  21. Koncic ZM, Kremer D, Karlovic K. Kosalec I. Evaluation of antioxidant activities and phenolic content of Berberis vulgaris L. and Berberis croatica Horvat. Food Chem. Toxicol. 48: 2176–2180 (2010)

    Article  Google Scholar 

  22. Ferreira ICFR, Baptista P, Vilas-Boas M, Barros L. Free radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: Individual cap and stipe activity. Food Chem. 100: 1511–1516 (2007)

    Article  CAS  Google Scholar 

  23. Mathew S, Abraham TE. In vitro antioxidant activity and scavenging effects of Cinnamomum verum leaves extract assayed by different methodologies Food Chem. Toxicol. 44: 198–206 (2006)

    CAS  Google Scholar 

  24. Shukla S, Mehta A, Bajpai KV, Shukla S. In vitro antioxidant activity and total phenolic content of ethanolic leaf extract of Stevia rebaudiana Bert. Food Chem. Toxicol. 47: 2338–2343 (2009)

    Article  CAS  Google Scholar 

  25. Wang J, Yuan X, Jin Z, Tian Y, Song H. Free radical and reactive oxygen species scavenging activities of peanut skins extract. Food Chem. 104: 242–250 (2007)

    Article  CAS  Google Scholar 

  26. Rauha JP, Remes S, Heinonen M, Hopia A, Kahkonen M, Kujala T, Pihlaja K, Vuorela H, Vuorela P. Antimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds. Int. J. Food Microbiol. 56: 3–12 (2000)

    Article  CAS  Google Scholar 

  27. Scherrer R, Gerhardt P. Molecular sieving by the Bacillus megaterium cell wall and protoplast. J. Bacteriol. 107: 718–735 (1971)

    CAS  Google Scholar 

  28. Pereira JA, Oliveira I, Sousa A, Valentao P, Andrade PB, Ferreira ICFR, Ferreres F, Bento A, Seabra R, Estevinho L. Walnut (Juglans regia L.) leaves: Phenolic compounds, antibacterial activity, and antioxidant potential of different cultivars. Food Chem. Toxicol. 45: 2287–2295 (2007)

    Article  CAS  Google Scholar 

  29. Nikaido H, Vaara M. Molecular basis of bacterial outer membrane permeability. Microbiol. Rev. 49: 1–32 (1985)

    CAS  Google Scholar 

  30. Gumus T. Determination of the changes of antifungal properties of Satureja hortensis, Thymus vulgaris, and Thymbra spicata exposed to γ-irradiation. Radiat. Phys. Chem. 79: 109–114 (2010)

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Hakime Hülya Orak.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Orak, H.H., Yagar, H., Isbilir, S.S. et al. Evaluation of antioxidant and antimicrobial potential of strawberry tree (Arbutus Unedo L.) leaf. Food Sci Biotechnol 20, 1249 (2011). https://doi.org/10.1007/s10068-011-0172-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s10068-011-0172-9

Keywords

Navigation