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Antioxidant and tyrosinase inhibitory activities of different parts of guava (Psidium guajava L.)

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Abstract

The antioxidant and the tyrosinase inhibitory activities of 4 different solvents (acetone, ethanol, methanol, and water) for preparation of extracts from guava (branch, fruit, leaf, and seed) were evaluated by measuring total phenolic contents (TPC), DPPH radical scavenging activity, ABTS radical scavenging activity, reducing power (RP), and tyrosinase inhibitory activity. The extracts of branch and leaf showed relatively higher antioxidant properties than those of fruit and seed. The highest TPC (141.28 mg/g gallic acid equivalents), DPPH radical scavenging activity (IC50=34.01 μg/mL), ABTS radical scavenging activity (IC50=3.23 μg/mL), and RP (IC50= 75.63 μg/mL) were found in acetone extract of leaf, while water extract of seed had the lowest antioxidant activity. The tyrosinase inhibitory activity of ethanol extract from guava leaf was 69.56%, which was the highest activity among the extracts. These results indicate that useful bioactive substances exist in the guava branch as well as leaf extracts.

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References

  1. Moskovitz J, Yim MB, Chock PB. Free radicals and disease. Arch. Biochem. Biophys. 397: 354–359 (2002)

    Article  CAS  Google Scholar 

  2. Duh PD. Antioxidant activity of burdock (Arctium lappa L.): Its scavenging effect on free radical and active oxygen. J. Am. Oil Chem. Soc. 75: 455–461 (1998)

    Article  CAS  Google Scholar 

  3. Lai LS, Chou ST, Chao WW. Studies on the antioxidative activities of hsian-tsao (Mesona procumbens Hemsl) leaf gum. J. Agr. Food Chem. 49: 963–968 (2001)

    Article  CAS  Google Scholar 

  4. Stangeland T, Remberg SF, Lye KA. Total antioxidant activity in 35 Ugandan fruits and vegetables. Food Chem. 113: 85–91 (2009)

    Article  CAS  Google Scholar 

  5. Mercadante AZ, Steck A, Pfander H. Carotenoids from guava (Psidium guajava L): Isolation and structure elucidation. J. Agr. Food Chem. 47: 145–151 (1999)

    Article  CAS  Google Scholar 

  6. Lutterodt GD. Inhibition of gastrointestinal release of acetylcholine by quercetin as a possible mode of action of Psidium guajava leaf extracts in the treatment of acute diarrhoeal disease. J. Ethnopharmacol. 25: 235–247 (1989)

    Article  CAS  Google Scholar 

  7. Olajide OA, Awe SO, Makinde JM. Pharmacological studies on the leaf of Psidium guajava. Fitoterapia 70: 25–31 (1999)

    Article  Google Scholar 

  8. Jaiarj P, Khoohaswan P, Wongkrajang Y, Peungvicha P, Suriyawong P, Saraya SML, Ruangsomboon O. Anticough and antimicrobial activities of Psidium guajava Linn, leaf extract. J. Ethnopharmacol. 67: 203–212 (1999)

    Article  CAS  Google Scholar 

  9. Matsuo T, Hanamure N, Shimoi K, Nakamura Y, Tomita I. Identification of (+)-gallocatechin as a bio-antimutagenic compound in Psidium guava leaves. Phytochemistry 36: 1027–1029 (1994)

    Article  CAS  Google Scholar 

  10. Gutfinger T. Polyphenols in olive oil. J. Am. Oil Chem. Soc. 58: 966–968 (1981)

    Article  CAS  Google Scholar 

  11. Jang IC, Oh WG, Ahn GH, Lee JH, Lee SC. Antioxidant activity of 4 cultivars of persimmon fruit. Food Sci. Biotechnol. 20: 71–77 (2011)

    Article  CAS  Google Scholar 

  12. Muller HE. Detection of hydrogen peroxide produced by microorganism on an ABTS peroxidase medium. Zbl. Bakt. -Int. J. Med. M. 259: 151–158 (1985)

    CAS  Google Scholar 

  13. Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Jpn. J. Nutr. 44: 307–315 (1986)

    CAS  Google Scholar 

  14. Vanni A, Gastaldi D, Giunata G. Kinetic investigations on the double enzymatic activity of the tyrosinase mushroom. Ann. Chim-Rome 80: 35–60 (1990)

    CAS  Google Scholar 

  15. Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Bio. Med. 20: 933–956 (1996)

    Article  CAS  Google Scholar 

  16. Naczk M, Grant S, Zadernowski R, Barre E. Protein precipitating capacity of phenolics of wild blueberry leaves and fruits. Food Chem. 96: 640–647 (2006)

    Article  CAS  Google Scholar 

  17. Liang Q, Quian H, Yao W. Identification of flavonoids and their glycosides by high-performance liquid chromatography with electrospray ionization mass spectrometry and with diode array ultraviolet detection. Eur. J. Mass Spectrom. 11: 93–101 (2005)

    Article  CAS  Google Scholar 

  18. Cvikrová M, Malá J, Hrubcová M, Eder J. Soluble and cell wall-bound phenolics and lignin in Ascocalyx abietina infected Norway spruces. Plant Sci. 170: 563–570 (2006)

    Article  Google Scholar 

  19. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199–1200 (1958)

    Article  CAS  Google Scholar 

  20. Jiménez-Escrig A, Rincon M, Pulido R, Saura-Calixto F. Guava fruit (Psidium guajava L.) as a new source of antioxidant dietary fiber. J. Agr. Food Chem. 49: 5489–5493 (2001)

    Article  Google Scholar 

  21. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying improved ABTS radical cation decolorization assay. Free Radical Bio. Med. 26: 1231–1237 (1999)

    Article  CAS  Google Scholar 

  22. Chen HY, Yen GC. Antioxidant activity and free radical-scavenging capacity of extracts from guava (Psidium guajava L.) leaves. Food Chem. 101: 686–694 (2007)

    Article  CAS  Google Scholar 

  23. Adedapo AA, Jimoh FO, Afolayan AJ, Masika PJ. Antioxidant activities and phenolic contents of the methanol extracts of the stems of Acokanthera oppositifolia and Adenia gummifera. BMC Complem. Altern. M. 8: 54–54 (2008)

    Article  Google Scholar 

  24. Yu L, Haley S, Perret J, Harris M, Wilson J, Qian M. Free radical scavenging properties of wheat extracts. J. Agr. Food Chem. 50: 1619–1624 (2002)

    Article  CAS  Google Scholar 

  25. Meir S, Kanner J, Akiri B, Philosoph-Hadas S. Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves. J. Agr. Food Chem. 43: 1813–1817 (1995)

    Article  CAS  Google Scholar 

  26. Vinson JA, Hontz BA. Phenol antioxidant index: Comparative antioxidant effectiveness of red and white wines. J. Agr. Food Chem. 43: 401–403 (1995)

    Article  CAS  Google Scholar 

  27. Kaur C, Kapoor HC. Anti-oxidant activity and total phenolic content of some Asian vegetables. Int. J. Food Sci. Tech. 37: 153–163 (2002)

    Article  CAS  Google Scholar 

  28. Miliauskas G, Venskutonis PR, Van Beek TA. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem. 85: 231–237 (2004)

    Article  CAS  Google Scholar 

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Correspondence to Seung-Cheol Lee.

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You, DH., Park, JW., Yuk, HG. et al. Antioxidant and tyrosinase inhibitory activities of different parts of guava (Psidium guajava L.). Food Sci Biotechnol 20, 1095–1100 (2011). https://doi.org/10.1007/s10068-011-0148-9

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  • DOI: https://doi.org/10.1007/s10068-011-0148-9

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