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Structural characteristics, fluorescence quenching, and antioxidant activity of the arabinogalactan protein-rich fraction from senna (Cassia angustifolia) leaves

  • Sharmistha Sinha
  • Shruti S. Bandyopadhyay
  • Debjani Ghosh
  • Udipta Ranjan Chatterjee
  • Sudipta Saha
  • Pradyot K. Ghosal
  • Bimalendu RayEmail author
Research Article

Abstract

Senna (Cassia angustifolia) is widely used in Indian folk medicine for the prevention of various disorders. Herein, we have demonstrated that water extracted arabinogalactan protein (AGP) rich fraction possesses strong antioxidative activity. The AGP rich fraction was analyzed using chemical, chromatographic, and spectroscopic methods. Effect of water extracted polymers on bovine serum albumin spectrum was determined using excitation wavelength of 282 nm. The antioxidant capacity of this fraction was studied by ferric reducing antioxidant power (FRAP) and DPPH radical assays. This polymeric fraction, ≥88% of which gets precipitated with Yariv reagent, consisted mainly of (1→5)-/(1→3,5)-linked α-arabinosyl, (1→3)-/(1→3,6)-linked-galactosyl residues and terminal arabinofuranosyl residues. Its in vitro antioxidant capacity is comparable to that of standard antioxidants. Fluorescence quenching studies furnishes evidence for the interaction of the arabinogalactan protein with bovine serum albumin.

Keywords

senna leaf (Cassia angustifoliaarabinogalactan protein fluorescence quenching antioxidant activity 

References

  1. 1.
    Finkel T, Holbrook NJ. Oxidants, oxidative stress, and the biology of aging. Nature 408: 239–247 (2000)CrossRefGoogle Scholar
  2. 2.
    Liu Z, Lin H, Ye S, Liu QY, Meng Z, Zhang C, Xia Y, Margoliash E, Rao Z, Liu XJ. Remarkably high activities of testicular cytochrome c in destroying reactive oxygen species and in triggering apoptosis. Biochemistry 103: 8965–8970 (2006)Google Scholar
  3. 3.
    Mucke L. Alzheimer’s disease. Nature 461: 495–497 (2009)CrossRefGoogle Scholar
  4. 4.
    Lee HB, Yu MR, Yang Y, Jiang Z, Ha H. Reactive oxygen species-regulated signaling pathways in diabetic nephropathy. J. Am. Soc. Nephrol. 14: 241–245 (2003)CrossRefGoogle Scholar
  5. 5.
    Paz-Elizur T, Sevilya Z, Leitner-Dagan Y, Elinger D, Roisman L, Livneh Z. DNA repair of oxidative DNA damage in human carcinogenesis: Potential application for cancer risk assessment and prevention. Cancer Lett. 266: 60–72 (2008)CrossRefGoogle Scholar
  6. 6.
    Schwarz K, Bertelsen G, Nissen LR, Gardner PT, Heinonen MI, Hopia A, Huynh-Ba T, Lambelet P, McPhail D, Skibsted LH, Tijburg L. Investigation of plant extracts for the protection of processed foods against lipid oxidation. Comparison of antioxidant assays based on radical scavenging, lipid oxidation, and analysis of the principal antioxidant compounds. Eur. Food Res. Technol. 212: 319–328 (2001)CrossRefGoogle Scholar
  7. 7.
    Waltenberger B, Avula B, Ganzera M, Khan IA, Stuppner H, Khan SI. Transport of sennosides and sennidines from Cassia angustifolia and Cassia senna across Caco-2 monolayers-an in vitro model for intestinal absorption. Phytomedicine 15: 373–377 (2008)CrossRefGoogle Scholar
  8. 8.
    Yadav JP, Arya V, Yadav S, Panghal M, Kumar S, Dhankhar S. Cassia occidentalis L.: A review on its ethnobotany, phytochemical, and pharmacological profile. Fitoterapia 81: 223–230 (2010)CrossRefGoogle Scholar
  9. 9.
    Berteau O, Mulloy B. Sulfated fucans, fresh perspectives: Structures, functions, and biological functions of sulfated fucans and an overview of enzymes active towards this class of polysaccharides. Glycobiology 13: 29–40 (2003)CrossRefGoogle Scholar
  10. 10.
    Chattopadhyay N, Ghosh T, Sinha S, Chattopadhyay K, Karmakar P, Ray B. Polysaccharides from Turbinaria conoides: Structural features and antioxidant capacity. Food. Chem. 11: 823–829 (2010)CrossRefGoogle Scholar
  11. 11.
    Chattopadhyay N, Nosalova G, Saha S, Bandyopadhyay SS, Fleskova D, Ray B. Structural features and antitussive activity of water extracted polysaccharide from Adhatoda vasica. Carbohyd. Polym. 83: 1970–1974 (2011)CrossRefGoogle Scholar
  12. 12.
    Ghosh T, Chattopadhyay K, Marschall M, Karmakar P, Mandal P, Ray B. Focus on antivirally active sulfated polysaccharides: From structure-activity analysis to clinical evaluation. Glycobiology 19: 2–15 (2009)CrossRefGoogle Scholar
  13. 13.
    Song H, Zhang Q, Zhang Z, Wang J. In vitro antioxidant activity of polysaccharides extracted from Bryopsis plumose. Carbohyd. Polym. 80: 1057–1061 (2010)CrossRefGoogle Scholar
  14. 14.
    Zhang Q, Li N, Zhou G, Lu X, Xu Z, Li Z. In vivo antioxidant activity of polysaccharide fraction from Porphyra haitanesis (Rhodephyta) in aging mice. Pharmacol. Res. 48: 151–155 (2003)CrossRefGoogle Scholar
  15. 15.
    Schultz CR, Johnson KL, Currie G, Bacic A. The classical arabinogalactan protein gene family of Arabidopsis. Cell 12: 1751–1767 (2000)Google Scholar
  16. 16.
    Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350–366 (1956)CrossRefGoogle Scholar
  17. 17.
    Ahmed A, Labavitch JM. A simplified method for accurate determination of cell wall uronide content. J. Food Biochem. 1: 361–365 (1977)CrossRefGoogle Scholar
  18. 18.
    Blakeney AB, Harris P, Henry RJ, Bruce AB. A simple rapid preparation of alditol acetates for monosaccharide analysis. Carbohyd. Res. 113: 291–299 (1983)CrossRefGoogle Scholar
  19. 19.
    York WS, Darvill A, O’Neill M, Stevenson T, Albersheim P. Isolation and characterisation of plant cell walls and cell wall components. Method. Enzymol. 118: 3–40 (1985)CrossRefGoogle Scholar
  20. 20.
    Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265–275 (1951)Google Scholar
  21. 21.
    Blakeney AB, Stone BA. Methylation of carbohydrates with lithium methylsulphinyl carbanion. Carbohyd. Res. 140: 319–324 (1985)CrossRefGoogle Scholar
  22. 22.
    Ray B. Polysaccharides from Enteromorpha compressa: Isolation, purification, and structural features. Carbohyd. Polym. 66: 408–416 (2006)CrossRefGoogle Scholar
  23. 23.
    Huang D, Ou B, Prior RL. The chemistry behind antioxidant capacity assays. J. Agr. Food Chem. 53: 1841–1856 (2005)CrossRefGoogle Scholar
  24. 24.
    Benzie IFF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Anal Biochem. 239: 70–76 (1996)CrossRefGoogle Scholar
  25. 25.
    Kacurakova M, Capek P, Sasinkova V, Wellner N, Ebringerova A. FT-IR study of plant cell wall model compounds: Pectic polysaccharides and hemicelluloses. Carbohyd. Polym. 43: 195–203 (2000)CrossRefGoogle Scholar
  26. 26.
    Capek P, Matulová M, Navarini L, Suggi-Liverani F. Structural features of an arabinogalactan-protein isolated from instant coffee powder of Coffea arabica beans. Carbohyd. Polym. 80: 180–185 (2010)CrossRefGoogle Scholar
  27. 27.
    Frankel EN, Meyer AS. The problems of using one-dimensional methods to evaluate multifunctional food and biological antioxidants. J. Sci. Food Agr. 80: 1925–1941 (2000)CrossRefGoogle Scholar
  28. 28.
    Moon JK, Shibamoto T. Antioxidant assays for plant and food components. J. Agr. Food Chem. 57: 1655–1666 (2009)CrossRefGoogle Scholar
  29. 29.
    Yuan XP, Wang J, Yao HY, Chen F. Free radical scavenging capacity and inhibitory activity on rat erythrocyte hemolysis of feruloyl oligosaccharides from wheat bran insoluble dietary fiber. LWT-Food Sci. Technol. 38: 877–883 (2005)CrossRefGoogle Scholar
  30. 30.
    Ou SY, Jackson GM, Jiao X, Chen J, Wu JZ, Huang XS. Protection against oxidative stress in diabetic rats by wheat bran feruloyl oligosaccharides. J. Agr. Food Chem. 55: 3191–3195 (2007)CrossRefGoogle Scholar
  31. 31.
    Wang J, Zhang Q, Zhang Z, Song H, Li P. Potential antioxidant and anticoagulant capacity of low molecular weight fucoidan fractions extracted from Laminaria japonica. Int. J. Biol. Macromol. 46: 6–12 (2010)CrossRefGoogle Scholar
  32. 32.
    Wang J, Zhang J, Wang X, Zhao B, Wu Y, Yao J. A comparison study on microwave-assisted extraction of Artemisia sphaerocephala polysaccharides with conventional method: Molecule structure and antioxidant activities evaluation. Int. J. Biol. Macromol. 45: 483–492 (2009)CrossRefGoogle Scholar
  33. 33.
    Zhang Q, Li N, Liu X, Zhao Z, Li Z, Xu Z. The structure of a sulfated galactan from Porphyra haitanensis and its in vivo antioxidant activity. Carbohyd. Res. 339: 105–111 (2004)CrossRefGoogle Scholar
  34. 34.
    Ruperez P, Ahrazem O, Leal A. Potential antioxidant capacity of sulphated polysaccharides from the edible marine brown seaweed Fucus vesiculosus. J. Agr. Food Chem. 50: 840–845 (2002)CrossRefGoogle Scholar
  35. 35.
    Qi H, Zhang Q, Zhao T, Hu R, Zhang K, Li Z. In vitro antioxidant activity of acetylated and benzoylated derivatives of polysaccharide extracted from Ulva pertusa (Chlorophyta). Bioorg. Med. Chem. 16: 2441–2445 (2006)CrossRefGoogle Scholar
  36. 36.
    Wang J, Zhang J, Zhao B, Wang X, Wu Y, Yao J. A comparison study on microwave-assisted extraction of Potentilla anserina L. polysaccharides with conventional method: Molecule weight and antioxidant activities evaluation. Carbohyd. Polym. 80: 84–93 (2010)CrossRefGoogle Scholar
  37. 37.
    Zhang Z, Wang F, Wang X, Liu X, Hou Y, Zhang Q. Extraction of the polysaccharides from five algae and their potential antioxidant activity in vitro. Carbohyd. Polym. 82: 118–121 (2010)CrossRefGoogle Scholar
  38. 38.
    Je J, Park P, Kim E, Park J, Yoon H, Kim K, Ahn C. Antioxidant activity of enzymatic extracts from the brown seaweed Undaria pinnatifida by electron spin resonance spectroscopy. LWT-Food Sci. Technol. 42: 874–878 (2009)CrossRefGoogle Scholar
  39. 39.
    Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Bio. Med. 20: 933–956 (1996)CrossRefGoogle Scholar
  40. 40.
    Peters T. Serum albumin. Adv. Protein Chem. 37: 161–245 (1985)CrossRefGoogle Scholar

Copyright information

© The Korean Society of Food Science and Technology and Springer Netherlands 2011

Authors and Affiliations

  • Sharmistha Sinha
    • 1
  • Shruti S. Bandyopadhyay
    • 1
  • Debjani Ghosh
    • 1
  • Udipta Ranjan Chatterjee
    • 1
  • Sudipta Saha
    • 1
  • Pradyot K. Ghosal
    • 1
  • Bimalendu Ray
    • 1
    Email author
  1. 1.Natural Products Laboratory, Department of ChemistryThe University of BurdwanBardhamanIndia

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