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α-Galactosidases production by Debaryomyces hansenii UFV-1

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Abstract

Extracellular and intracellular α-galactosidases were produced by yeast Debaryomyces hansenii UFV-1 grown on different media with several carbon sources. D. hansenii grown in YP-medium (1% yeast extract and 2% peptone) presented maximum cell mass (8.45 mg/mL) after 36 h of cultivation, with lactose as carbon source, followed by sucrose, glucose, raffinose, and galactose. Higher extracellular and intracellular α-galactosidases activities were observed at 48 h of D. hansenii cultivation in YPmedium containing galactose (0.97 and 5.27 U/mL) and lactose (1.28 and 4.88 U/mL), supporting the evidence for the model of induction for the yeast GAL/MEL regulon, such as described in Sacharomyces cereviseae.

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Authors and Affiliations

  1. BIOAGRO, Universidade Federal de Viçosa, Campus Viçosa, Viçosa, MG, 36570-000, Brazil

    Pollyanna Amaral Viana, Sebastião Tavares de Rezende, Flávia Maria Lopes Passos, Solimar Gonçalves Machado, Gabriela Picollo Maitan & Valéria Monteze Guimarães

  2. Campus Florestal, Universidade Federal de Viçosa, Florestal, MG, 35690-000, Brazil

    Pollyanna Amaral Viana

  3. Departamento de Bioquímica e Imunologia-ICB, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil

    Vinicio Tadeu da Silva Coelho

Authors
  1. Pollyanna Amaral Viana
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  2. Sebastião Tavares de Rezende
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  3. Flávia Maria Lopes Passos
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  7. Valéria Monteze Guimarães
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Correspondence to Pollyanna Amaral Viana.

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Viana, P.A., de Rezende, S.T., Passos, F.M.L. et al. α-Galactosidases production by Debaryomyces hansenii UFV-1. Food Sci Biotechnol 20, 601–606 (2011). https://doi.org/10.1007/s10068-011-0085-7

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  • DOI: https://doi.org/10.1007/s10068-011-0085-7

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