Food Science and Biotechnology

, Volume 20, Issue 1, pp 159–165 | Cite as

Influence of extra virgin olive oil on the formation of heterocyclic amines in roasted beef steak

  • Jiyeong Lee
  • Ahyoung Dong
  • Kyunghee Jung
  • Han-Seung Shin
Research Article

Abstract

In this study, heterocyclic anime (HCA) contents were monitored in commonly consumed pan-fried beefsteak based on the highest level of human exposure. Effect of addition of extra virgin olive oil (EVOO) on HCAs formation in fried beef steaks was evaluated. After EVOO was spread on the meat surface, the raw beef was cooked at 200°C for 5 min on each side. The HCAs were extracted from the meat samples and purified using a solid-phase extraction method and then analyzed by liquid chromatography-mass spectrometry (LC-MS). Among the 15 HCAs, 3-amino-1,4-dimethyl-5H-pyrido-[4,3-b]indole (Trp-P-1), 3-amino-1-methyl-5H-pyrido [4,3-b]indole (Trp-P-2), 9H-pyrido [3,4-b]indole (Norharman), 1-methyl-9H-pyrido [3, 4-b]indole (Harman), 2-amino-9H-pyrido [2,3-b]indole (AαC), 2-amino-3-methyl-9H-pyrido [2,3-b]indole (MeAαC), 2-amino-3,8-dimethylimidazo [4,5-f]-quinoxaline (MeIQx), and 2-amino-1-methyl-6-phenylimidazo [4,5-b]-pyridine (PhIP) were detected in all of the cooked beefsteaks. HCAs formation was significantly reduced (p<0.05) when the EVOO was added to the beef prior to cooking. The addition of 2 and 4 g of EVOO considerably inhibited HCAs formation in the fried beefsteak. However, adding excess amounts of EVOO promoted some HCAs formation.

Keywords

heterocyclic amine extra virgin olive oil roasted beef pathy cooking 

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Copyright information

© The Korean Society of Food Science and Technology and Springer Netherlands 2011

Authors and Affiliations

  • Jiyeong Lee
    • 1
  • Ahyoung Dong
    • 1
  • Kyunghee Jung
    • 1
  • Han-Seung Shin
    • 1
  1. 1.Department of Food Science and Biotechnology and Institute of Lotus Functional Food IngredientsDongguk University-SeoulSeoulKorea

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