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Antimicrobial activity of Myagropsis myagroides and interaction with food composition

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Abstract

Ethanol extract of Myagropsis myagroides (MMEE) has substantial antimicrobial activity against Gram-positive bacteria and some of the yeasts. The minimum inhibitory concentration (MIC) of MMEE ranged from 0.0625 to 0.125 mg/mL for Bacillus subtilis, Listeria monocytogenes, and Clostridium perfringens. Chloroform fraction obtained through liquid-liquid extraction possessed the strongest antimicrobial activity, and was separated by silica gel column chromatography. Among 22 of subfraction, CH4 showed the strongest activity (4–8 times MIC of the crude extract). The antimicrobial activity of CH4 to Staphylococcus aureus was reduced at high concentrations of starch, but the bactericidal effect of CH4 was maintained at 1 and 5% starch model media. The efficacy of CH4 against S. aureus diminished in the presence of >5% beef extract and >1% soybean oil. In acute toxicity test, no mortalities occurred in mice administered 5 g/kg body weight of MMEE over 2 weeks observation.

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References

  1. Hannuksela M, Haahtela T. Hypersensitivity reactions to food additives. Allergy 42: 561–575 (1987)

    Article  CAS  Google Scholar 

  2. Food and Drug Administration. Data on benzene in soft drinks and other beverages. Available from: http://www.fda.gov/Food/FoodSafety/FoodContaminants Adulteration/Chemical Contaminants/Benzene/ucm055815.htm. Accessed Dec. 10, 2009.

  3. Piper PW. Yeast superoxide dismutase mutants reveal a pro-oxidant action of weak organic acid food preservatives. Free Radical Bio. Med. 27: 1219–1227 (1999)

    Article  CAS  Google Scholar 

  4. Cho SH, Lee SY, Kim JW, Ko GH, Seo IW. Development and application of natural antimicrobial agent isolated from grapefruit seed extract-antimicrobial activities of grapefruit seed extract. J. Food Hyg. Saf. 10: 33–39 (1995)

    Google Scholar 

  5. Jamuna M, Jeevaratnam K. Antibacterial efficacy of nisin and pediocins against food spoilage and pathogenic organisms in broth and a vegetarian food. J. Food Sci. Technol. 46: 563–568 (2009)

    CAS  Google Scholar 

  6. Hughey VL, Johnson EA. Antimicrobial activity of lysozyme against bacteria involved in food spoilage and food-borne disease. Appl. Environ. Microb. 53: 2165–2170 (1987)

    CAS  Google Scholar 

  7. Nagayama K, Iwamura Y, Shibara T, Hirayama I, Nakamura T. Bactericidal activity phlorotannins from the brown alga Ecklonia kurome. J. Antimicrob. Chemoth. 50: 889–893 (2002)

    Article  CAS  Google Scholar 

  8. Lee SY, Kim JH, Song E-J, Kim KBWR, Hong YK, Lim SM, Ahn DH. Investigation of antimicrobial activity of brown algae extracts and the thermal and pH effects on their activity. Food Sci. Biotechnol. 18: 506–512 (2009)

    CAS  Google Scholar 

  9. Lim CW, Lee JS, Cho YJ. Structure and some properties of the antimicrobial compounds in the red alga, Symphyosladia latiscula. J. Korean Fish Soc. 33: 280–287 (2000)

    CAS  Google Scholar 

  10. Awad NE, Selim MA, Saleh MM, Matloub AA. Seasonal variation of the lipoidal matters and hypolipidaemic activity of the red algae Corallina officinalis L. Phytother. Res. 17: 19–25 (2003)

    Article  CAS  Google Scholar 

  11. Kang JY, Khan MNA, Park NH, Cho JY, Lee MC, Fujii H, Hong YK. Antipyretic, analgesic, and anti-inflammatory activities of the seaweed Sargassum fulvellum and Sargassum thunbergii in mice. J. Ethnopharmacol. 116: 187–190 (2008)

    Article  CAS  Google Scholar 

  12. Iwashima M, Mory J, Xiang T, Matsunaga T, Hayashi K, Shinoda D, Saito H, Sankawa U, Hayashi T. Antioxidant and antiviral activities of plastoquinones from the brown alga Sargassum micracathum, and a new chromene derivative converted from the plastoquinones. Biol. Pharm. Bull 28: 374–377 (2005)

    Article  CAS  Google Scholar 

  13. Gamal-Eldeen AM, Ahmed EF, Abo-Zeid MA. In vitro cancer chemopreventive properties of polysaccharide extract from the brown alga, Sargassum latifolium. Food Chem. Toxicol. 47: 1378–1384 (2009)

    Article  CAS  Google Scholar 

  14. Kuda T, Kunii T, Goto H, Suzuki T, Yano T. Varieties of antioxidant and antibacterial properties of Ecklonia stolonifera and Ecklonia kurome products harvested and processed in the Noto peninsula, Japan. Food Chem. 103: 900–905 (2007)

    Article  CAS  Google Scholar 

  15. Segawa M, Shirahama H. New plastoquinones from the brown alga Sargassum sagamianum var. yezoense. Chem. Lett. 1: 1365–1366 (1987)

    Article  Google Scholar 

  16. Kato T, Kumanireng AS, Ichinose I, Kitahara Y, Kato Y. Active components of Sargassum tortile effecting the settlement of swimming larvae of Coryne uchidai. Experientia 31: 433–434 (1975)

    Article  CAS  Google Scholar 

  17. Nakayama M, Fukuoka Y, Nozaki H, Matsuo A, Hayashi S. Structure of (+) kjellmanianone, a highly oxygenated cyclopentenone from the marin alga Sargassum kjellmanianum. Chem. Lett. 9: 1243–1245 (1980)

    Article  Google Scholar 

  18. Burt S. Essential oils and their antibacterial properties and potential applications in foods-A review. Int. J. Food Microbiol. 94: 223–253 (2004)

    Article  CAS  Google Scholar 

  19. Lemos TLG, Motos FJA, Alencar JW, Craveiro AA, Clark AM, McCheesney JD. Antimicrobial activity of essential oils of Brasillian plants. Phytogher. Res. 4: 82–88 (1990)

    Article  Google Scholar 

  20. Nakamura S, Kato AM, Kobayashi K. New antimicrobial characteristics of lysozyme-dextran conjugate. J. Agr. Food Chem. 39: 647–650 (1991)

    Article  CAS  Google Scholar 

  21. EI-Shenawy MA, Marth EH. Inhibitory or inactivation of Listeria monocytogenes by sodium benzoate together with some organic acid. J. Food Protect. 52: 771–776 (1989)

    Google Scholar 

  22. Cava R, Nowak E, Taboada A, Marin-Iniesta F. Antimicrobial activity of clove and cinnamon essential oils against Listeria monocytogenes in pasteurized milk. J. Food Protect. 70: 2757–2763 (2007)

    CAS  Google Scholar 

  23. Glass KA, Johnson EA. Antagonistic effect of fat on the antiboltulinal activity of food preservatives and fatty acids. Food Microbiol. 21: 675–682 (2004)

    Article  CAS  Google Scholar 

  24. Rico-Mounz E, Davidson PM. Effect of corn oil and casein on the antimicrobial activity of phenolic antioxidants. J. Food Sci. 48: 1284–1288 (1983)

    Article  Google Scholar 

  25. Pol IE, Mastwijk HC, Slump RA, Popa ME, Smid EJ. Influence of food matrix on inactivation of Bacillus cereus by combinations of nisin, pulsed electric field treatment and carvacrol. J. Food Protect. 64: 1012–1018 (2001)

    CAS  Google Scholar 

  26. Devlieghere F, Vermeulen A, Debevere J. Chitosan: Antimicrobial actitvity, interactions with food components and applicability as a coating on fruit and vegetables. Food Microbiol. 21: 703–714 (2004)

    Article  CAS  Google Scholar 

  27. Gill AO, Delaquis P, Russo P, Holley RA. Evaluation of antilisterial action of cilantro oil on vaccum packed ham. Int. J. Food Microbiol. 73: 83–92 (2002)

    Article  CAS  Google Scholar 

  28. Aureli P, Costantini A, Zolea S. Antimicrobial activity of some plant essential oils against Listeria monocytogens. J. Food Protect. 55: 344–348 (1992)

    Google Scholar 

  29. Pandit VA, Shelef LA. Sensitivity of Listeria monocytogens to rosemary (Rosmarinus officinalis L.). Food Microbiol. 11: 57–63 (1994)

    Article  Google Scholar 

  30. Shelef LA, Jyothi EK, Bulgarelli MA. Growth of enteropathogenic and spoilage bacteria in sage-containing broth and foods. J. Food Sci. 49: 737–740 (1984)

    Article  Google Scholar 

  31. Chipley JR. Sodium benzoate and benzoic acid. pp. 11–48. In: Antimcirobials in Food. Dacidson PM, Sofos JN, Barnen AL (eds). CRC Press, New York, NY, USA (2004)

    Google Scholar 

  32. The Working Group on the Safety and Efficacy of Herbal Medicine of WHO. Research Guidelines for Evaluating the Safety and Efficacy of Herbal Medicine. 1st ed. Regional Office for Western Pacific, Manila, Philippines. pp. 35–42 (1993)

    Google Scholar 

  33. Brock WD, Trochimowicz HJ, Millischer RJH, Farr D, Kawano T, Rusch GM. Acute and subchronic toxicity of 1,1-dichloro-1-fluoethane (HCFC-141 b). Food Chem. Tocicol. 33: 483–490 (1995)

    Article  CAS  Google Scholar 

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Lee, SY., Song, EJ., Kim, KBWR. et al. Antimicrobial activity of Myagropsis myagroides and interaction with food composition. Food Sci Biotechnol 20, 85–92 (2011). https://doi.org/10.1007/s10068-011-0012-y

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