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Properties of nanopowdered chitosan and its cholesterol lowering effect in rats

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This study was carried out to compare the properties of nanopowdered chitosan (NPC) to commercially powdered chitosan (CPC) and examine the effect of NPC on serum and liver cholesterol lowering in rats. Twentyfour male rats of Sprague-Dawley strain were blocked into 3 groups and were raised for 8 weeks. High cholesterol diet, 2% CPC, or 2% NPC were given to 3 groups. NPC reduced total cholesterol by 46.6% and CPC reduced it by 18.6%. NPC also significantly reduced low density lipoprotein (LDL)-cholesterol by 55.7%, while CPC reduced by 36.8%. High density lipoprotein (HDL)-cholesterol content was increased more with NPC by 16.5% than with CPC by 9.6%. Triacylglycerol content was also decreased significantly by 42.8% with NPC, while reduced by 22.5% with CPC. The present study indicated that NPC feeding reduced total and LDL-cholesterol and triacylglycerol contents and increased HDL-cholesterol content.

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  1. Razdan A, Pettersson D. Effect of chitin and chitosan on nutrient digestibility and plasma lipid concentrations in broiler chickens. Brit. J. Nutr. 72: 277–288 (1994)

    Article  CAS  Google Scholar 

  2. Sugano M, Watanabe S, Kishi A, Izume M, Ohtakara A. Hypocholesterolemic action of chitosans with different viscosity in rats. Lipids 23: 187–191 (1988)

    Article  CAS  Google Scholar 

  3. Jenning CD, Boleyn K, Bridges SR, Wood PJ, Anderson JW. A comparison of the lipid-lowering and intestinal morphological effects of cholestyramine, chitosan, and oat gum in rats. Proc. Soc. Exp. Biol. Med. 189: 13–20 (1988)

    Google Scholar 

  4. Sugano M, Fujikawa T, Hiratsuji Y, Nakashima K, Furada N, Hasegawa YA. A novel use of chitosan as a hypocholesterolemic agent in rats. Am. J. Clin. Nutr. 33: 787–793 (1980)

    CAS  Google Scholar 

  5. Ikeda I, Sugano M, Yoshida K, Sasaki E, Iwamoto Y, Hatano K. Effects of chitosan hydrolyzates on lipid absorption and on serum and liver concentration in rats. J. Agr. Food Chem. 41: 431–435 (1993)

    Article  CAS  Google Scholar 

  6. Simunek J, Bartonova H. Effect of dietary chitin and chitosan on cholesterolemic of rats. Acta Vet.Brno 74: 491–499 (2005)

    Article  CAS  Google Scholar 

  7. Guerciolini R, Radu-Radulescu L, Boldrin M, Dallas J, Moore R. Comparative evaluation of fecal excretion induced by orlistat and chitosan. Obes. Res. 9: 364–367 (2001)

    Article  CAS  Google Scholar 

  8. Maezaki Y, Tsuji K, Nakagawa Y, Kawai Y, Akimoto M, Tsugita T. Hypocholesterolemic effect of chitosan in adult males. Biosci. Biotech. Bioch. 57: 1439–1444 (1993)

    Article  CAS  Google Scholar 

  9. Ventura P. Lipid lowering activity of chitosan, a new dietary integrator. Vol. 2, pp. 55–62. In: Chitin Enzymology. Muzarelli RAA (ed). Atec Edizioni, Ancona, Italy (1996)

    Google Scholar 

  10. LeHoux JG, Grondin F. Some effects of chitosan on liver function in the rat. Endocrinology 132: 1078–1084 (1993)

    Article  CAS  Google Scholar 

  11. Hegsted DM, Ausman L, Johnson JA, Dallal GE. Dietary fat and serum lipids: An evaluation of the experimental data. Am. J. Clin. Nutr. 57: 785–883 (1993)

    Google Scholar 

  12. Flegal KM, Carroll MD, Kuezmaski RJ, Johnson CL. Overweight and obesity in the United States: Prevalence and trends, 1960–94. Int. J. Obes. Relat. Metab. Disord. 22: 39–47 (1998)

    Article  CAS  Google Scholar 

  13. Troiano RP, Flegal KM. Overweight children and adolescents; Description, epidemiology, and demographics. Pediatrics 101: 497–504 (1998)

    CAS  Google Scholar 

  14. Metso S, Ylitalo R, Nikkilä M, Wuolijoki E, Ylitalo P, Lehtimäki T. The effect of long-term microccystalline chitosan therapy on plasma lipids and glucose concentrations in subjects with increased plasma total cholesterol: A randomized placebo-controlled double-blind crossover trial in healthy men and women. Eur. J. Clin. Pharmacol. 59: 741–746 (2003)

    Article  CAS  Google Scholar 

  15. Ylitalo R, Lehtinen S, Wuolijoki E, Lehtimaki T. Cholesterollowering properties and safety of chitosan. Arznei. Forschung 52: 1–7 (2002)

    CAS  Google Scholar 

  16. Chiang MT, Yao HT, Chen HC. Effect of dietary chitosans with different viscosity on plasma lipids and lipid peroxidation in rats fed in a diet enriched with cholesterol. Biosci. Biotech. Bioch. 64: 965–971 (2000)

    Article  CAS  Google Scholar 

  17. Rasenack N, Muller BW. Micron-size drug particle: Common and novel micronization techniques. Pharm. Dev. Technol. 9: 1–13 (2004)

    Article  CAS  Google Scholar 

  18. AOAC. Official Method of Analysis of AOAC. 40th ed. Method 948.12. Association of Official Analytical Chemists, Arlington, VA, USA (1990)

    Google Scholar 

  19. Sannan T, Kurita K, Ogura K, Iwakura Y. Studies on chitin: 7. IR spectroscopic determination of degree of deacetylation. Polymer 19: 458–459 (1997)

    Article  Google Scholar 

  20. No KH, Park NY, Lee SH, Meyers SP. Antibacterial activity of chitosans and chitosan oligomers with different molecular weights. Int. J. Food Microbiol. 74: 65–72 (2002)

    Article  CAS  Google Scholar 

  21. Seo MH, Lee SY, Chang YH, Kwak HS. Physicochemical, microbial, and sensory properties of yogurt supplemented with nanopowdered chitosan during storage. J. Dairy Sci. 92: 5907–5916 (2009)

    Article  CAS  Google Scholar 

  22. Liu J, Zhang J, Xia W. Hypocholesterolemic effects of different chitosan samples in vitro and in vivo. Food Chem. 107: 419–425 (2008)

    Article  CAS  Google Scholar 

  23. Deuchi K, Kanauchi O, Shizukuishi M, Kobayashi E. Continuous and massive intake of chitosan affects mineral and fat-soluble vitamin status in rats fed on a high-fat diets. Biosci. Biotech. Bioch. 59: 1211–1216 (1995)

    Article  CAS  Google Scholar 

  24. Trautwein EA, Jürgensen U, Erbersdobler HF. Cholesterol-lowering and gallstone-preventing action of chitosans with different degrees of deacetylation in hamsters fed cholesterol-rich diets. Nutr. Res. 17: 1053–1065 (1997)

    Article  CAS  Google Scholar 

  25. Hossain S, Rahman A, Kabir Y, Shams AA, Afros F, Hashimoto M. Effect of shrimp (Macrobracium RosenbergII)-derived chitosan on plasma lipid profile and liver lipid peroxide levels in normo- and hypercholesterolaemic rats. Clin. Exp. Pharmacol. P. 34: 170–176 (2007)

    Article  CAS  Google Scholar 

  26. Zhang J, Liu J, Li L, Xia W. Dietary chitosan improves hypocholesterolmia in rats fed high-fat diets. Nutr. Res. 28: 383–390 (2008)

    Article  CAS  Google Scholar 

  27. Vahouny GV, Satchihanandam S, Cassidy MM, Lightfoot FB, Furda I. Comparative effects of chitosan and cholestyramine on lymphatic absorption of lipids in the rats. Am. J. Clin. Nutr. 38: 278–284 (1983)

    CAS  Google Scholar 

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Correspondence to Hae-Soo Kwak.

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Park, J.H., Hong, EK., Ahn, J. et al. Properties of nanopowdered chitosan and its cholesterol lowering effect in rats. Food Sci Biotechnol 19, 1457–1462 (2010).

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