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Cytoprotective activity of lotus (Nelumbo nucifera Gaertner) leaf extracts on the mouse embryonic fibroblast cell

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Abstract

This study was conducted to evaluate the cytoprotective activity of lotus (Nelumbo nucifera Gaertner) leaf extract (LLE) on mouse embryonic fibroblast (MEF) cells. The 2-diphenyl-1-picrylhydrazyl hydrate (DPPH) free radical scavenging activities of LLE increased in a concentration dependent manner. The cells, damaged by oxidative stress, decreased their viability following increasing concentration of H2O2, but the co-treatment of n-butanol fraction of LLE and H2O2 resulted in an increase in cell growth, by about 25%, compared to the cells treated with H2O2. The n-butanol fraction of LLE inhibited the cytotoxicity induced by H2O2 in a concentration dependent manner. The oxidative damage to the cells, measured by apoptotic and necrotic cell accumulation, was similar with the addition of the n-butanol fraction of LLE to H2O2. Taken together, these results suggest that LLE inhibited the cytotoxicity which is induced by H2O2, and has a protective effect on MEF cell against oxidative stress.

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References

  1. Choi HY, Jung KH, Shin HS. Antioxidant activity of the various extracts from different parts of lotus (Nelumbo nucifera Gaertner). Food Sci. Biotechnol. 18: 1051–1054 (2009)

    CAS  Google Scholar 

  2. Lui RH. Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. Am. J. Clin. Nutr. 78: 517–520 (2003)

    Google Scholar 

  3. Buxiang S, Fukuhara M. Effects of co-administration of butylated hydroxytoluene, butylated hydroxyanisole, and flavonoide on the activation of mutagens and drug-metabolizing enzymes in mice. Toxicology 122: 61–72 (1997)

    Article  Google Scholar 

  4. Namiki M. Antioxidants/antimutagens in food. Crit. Rev. Food Sci. 29: 273–300 (1990)

    Article  CAS  Google Scholar 

  5. Anonymous. The Wealth of India — A Dictionary of Indian Raw Materials. Vol. 7. Council of Scientific Industrial Reasearch, New Delhi, India. pp. 7–11 (1996)

  6. Sridhar KR, Bhat R. Lotus-a potential nutraceutical source. J. Agr. Technol. 3: 143–155 (2007)

    Google Scholar 

  7. Yu J, Hu WS. Effects of neferine on platelet aggregation in rabbits. Yaoxue Xuebao 32: 1–4 (1997)

    CAS  Google Scholar 

  8. Kim SB, Rho SB, Rhyu DY, Kim DW. Effect of Nelumbo nucifera leaves on hyperlipidemic atherosclerotic Bio F1B hamster. Korean J. Pharmacogn. 36: 229–234 (2005)

    Google Scholar 

  9. Kashiwada Y, Aoshima A, Ikeshiro Y, Chen YP, Furukawa H, Itoigawa M, Fujioka T, Misashi K, Mark Cosemtino L, Morris-Natschke SL, Lee KH. Anti-HIV benzylisoquinoline alkaloids and flavonoids from the leaves of Nelumbo nucifera, and structureactivity correlations with related alkaloids. Bioorg. Med. Chem. 10: 443–448 (2005)

    Article  Google Scholar 

  10. Ono Y, Hattori E, Fukaya Y, Imai S, Ohizumi Y. Anti-obesity effect of Nelumbo nucifera leaves extract in mice and rats. J. Ethnopharmacol. 106: 238–244 (2006)

    Article  Google Scholar 

  11. Cho EJ, Yokozawa T, Rhyu DY, Kim SC, Shibahara N, Park JC. Study on the inhibitory effects of Korean medicinal plants and their main compounds on the 1,1-diphenyl-2-picrylhydrazyl radical. Pytomedicine 10: 544–551 (2003)

    Article  CAS  Google Scholar 

  12. Wu MJ, Wang L, Weng CY, Yen JH. Antioxidant activity of methanol extract of the lotus leaf (Nelumbo nucifera Gertn.). Am. J. Chin. Med. 31: 687–698 (2003)

    Article  Google Scholar 

  13. Lin HY, Kuo YH, Lin YL, Chiang W. Antioxidantive effect and active components from leaves of lotus (Nelumbo nucifera) J. Agr. Food Chem. 57: 6623–6629 (2008)

    Article  Google Scholar 

  14. AOAC. Official Methods of Analysis. 15th ed. Association of Official Analytical Chemists, Washington, DC, USA. p. 31 (1980)

    Google Scholar 

  15. Korea Food & Drug Administration. Food Code. Seoul, Korea (2008)

  16. Lee OH, Lee HB, Son JY. Antimicrobial activities and nitritescavenging ability of olive leaf fractions. Korean J. Soc. Food Cookery Sci. 20: 204–210 (2004)

    Google Scholar 

  17. Lee KS, Kwon YJ, Lee KY. Analysis of chemical composition, vitamin, mineral, and antioxidative effect of the lotus leaf. Korean J. Soc. Food Cookery Sci. 12: 1622–1626 (2008)

    Article  Google Scholar 

  18. Park BH, Cho HS, Jeon ER, Kim SD, Koh KM. Quality characteristics of soybean curd prepared with lotus leaf powder. Korean J. Food Culture 24: 315–320 (2009)

    Google Scholar 

  19. Kim KS, Shin MK, Kim HY. Nutritional composition and antioxidant activity of the white lotus (Nelumbo nucifera Gaertn) leaf. J. East Asian Soc. Dietary Life 18: 499–506 (2008)

    Google Scholar 

  20. Yang HC, Heo NC, Choi KC, Ahn YJ. Nutritional composition of white-flowered and pink-flowered lotus in different parts. Korean J. Food Sci. Technol. 39: 14–19 (2007)

    Google Scholar 

  21. Yoon SJ, Noh KS. The effect of lotus leaf powder on the quality of dasik. Korean J. Food Cookery Sci. 25: 25–30 (2009)

    Google Scholar 

  22. Saenghhae C, Arunnopparat W, Sungkhajorn P. Antioxidative activity of the leaf of Nelumbo nucifera Gaertn. on oxidative stressinduced erythrocyte hemolysis in hypertensive and normotensive rats. Thai J. Phys. Sci. 20: 70–78 (2008)

    Google Scholar 

  23. Mingyu Li, Zhuting Xu. Quercetin in a lotus leaves extract may be responsible for antibacterial activity. Arch. Pharm. Res. 31: 640–644 (2008)

    Article  CAS  Google Scholar 

  24. Jung HA, Jung YJ, Yoon NY, Jeong DM, Bae HJ, Kim DW, Na DH, Choi JS. Inhibitory effects of Nelumbo nucifera leaves on rat lens aldose reductase, advanced glycation endproducts formation, and oxidative stress. Food Chem. Toxicol. 46: 3818–3826 (2008)

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Food Science and Biotechnology and Institute of Lotus Functional Food Ingredients, Dongguk University-Seoul, Seoul, 100-715, Korea

    Ju Yeon Ha, Kyueun Lee, Jeongmi Park, Ahyoung Dong & Han-Seung Shin

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  1. Ju Yeon Ha
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  2. Kyueun Lee
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  3. Jeongmi Park
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  4. Ahyoung Dong
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  5. Han-Seung Shin
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Correspondence to Han-Seung Shin.

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Ha, J.Y., Lee, K., Park, J. et al. Cytoprotective activity of lotus (Nelumbo nucifera Gaertner) leaf extracts on the mouse embryonic fibroblast cell. Food Sci Biotechnol 19, 1171–1176 (2010). https://doi.org/10.1007/s10068-010-0167-y

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