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Dietary supplementation of red ginseng, Chinese lizard’s tail, and Korean raspberry extracts alleviate oxidative stresses in aged rats

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Abstract

To investigate antioxidant efficacy of medicinal plants known to possess anti-aging properties, the herb preparations were fed to 10-month-old rats. The herb preparations included ethanol extracts of red ginseng (Panax ginseng C.A. Mayer), Chinese lizard’s tail (Saururus chinensis Hort.ex Loud), and Korean raspberry (fruits of Rubus coreanus Miquel). Plasma protein carbonyls, malondialdehyde, 8-iso-prostaglandinF2α, lymphocyte DNA damage, α-tocopherol concentration, and ferric reducing ability were measured as oxidative stress markers. The aged rats showed higher levels of malondialdehyde, 8-isoprostaglandinF2α, and protein carbonyl and lower level of α-tocopherol compared to young rats. Malondialdehyde and protein carbonyl levels were decreased in the aged rats fed diets supplemented with any of the herbal preparations. 8-Iso-prostaglandinF2α was lower in rats fed the 0.5 or 1.0% Chinese lizard’s tail- or 1% Korean raspberry extract-supplemented diet. The 1:1:1 mixture of herbal preparations decreased the level of 8-iso-prostaglandinF2α. Rats fed 1.0% Chinese lizard’s tail or Korean raspberry diets showed increased α-tocopherol level. These results indicated that the level of oxidative stress is increased in the aged animals and herbal preparations are effective in the alleviation of age-related oxidation.

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References

  1. Droge W. Free radicals in the physiological control of cell function. Physiol. Rev. 82: 47–95 (2002)

    CAS  Google Scholar 

  2. Halliwell B. Antioxidants and human disease: A general introduction. Nutr. Rev. 55: S44–S49 (1997)

    Article  CAS  Google Scholar 

  3. Polidori MC, Savino K, Alunni G, Freddio M, Senin U, Sies H, Stahl W, Mecocci P. Plasma lipophilic antioxidants and malondialdehyde in congestive heart failure patients: Relationship to disease severity. Free Radical. Bio. Med. 32: 148–152 (2002)

    Article  CAS  Google Scholar 

  4. Astrup A, Dyerberg J, Selleck M, Stender S. Nutrition transition and its relationship to the development of obesity and related chronic diseases. Obes. Rev. 9(Suppl 1): 48–52 (2008)

    Article  Google Scholar 

  5. Baker H. Nutrition in the elderly: Nutritional aspects of chronic disease. Geriatrics 62: 21–25 (2007)

    Google Scholar 

  6. Herber D. Vegetables, fruits, and phytoesterogens in the prevention of disease. J. Postgrad. Med. 50: 145–149 (2004)

    Google Scholar 

  7. Keum YS, Park KK, Lee JM, Chun KS, Park JH, Lee SK, Kwon H, Surh YJ. Antioxidant and anti-tumor promoting activities of the methanol extract of heat-processed ginseng. Cancer Lett. 150: 41–48 (2000)

    Article  CAS  Google Scholar 

  8. Kim YK, Guo Q, Packer L. Free radical scavenging activity of red ginseng aqueous extracts. Toxicology 172: 149–156 (2002)

    Article  CAS  Google Scholar 

  9. Kitts D, Hu C. Efficacy and safety of ginseng. Public Health Nutr. 3: 473–485 (2000)

    Article  CAS  Google Scholar 

  10. Kumar M, Sharma MK, Saxena PS, Kumar A. Radioprotective effect of Panax ginseng on the phosphatases and lipid peroxidation level in testes of Swiss albino mice. Biol. Pharm. Bull. 26: 308–312 (2003)

    Article  CAS  Google Scholar 

  11. Chung BS, Shin MG. Dictionary of Korean Folk Medicine. Young Lim Sa, Seoul, Korea. p. 813 (1990)

    Google Scholar 

  12. Sadhu SK, Okuyama E, Fujimoto H, Ishibashi M. Separation of Leucas aspera, a medicinal plant of Bangladesh, guided by prostaglandin inhibitory and antioxidant activities. Chem. Pharm. Bull. 51: 595–598 (2003)

    Article  CAS  Google Scholar 

  13. Yoon I, Wee JH, Moon JH, Ahn TH, Park KH. Isolation and identification of quercetin with antioxidative activity from the fruits of Rubus coreanus Miquel. Korean J. Food Sci. Technol. 35: 499–502 (2003)

    Google Scholar 

  14. Mayne ST. Antioxidant nutrients and chronic disease: Use of biomarkers of exposure and oxidative stress status in epidemiologic research. J. Nutr. 133(Suppl 3): 933S–940S (2003)

    CAS  Google Scholar 

  15. AIN. Report of the American Institute of Nutrition ad hoc committee on standards for nutritional studies. J. Nutr. 107: 1340–1348 (1977)

    Google Scholar 

  16. Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 175: 184–191(1988)

    Article  CAS  Google Scholar 

  17. Bieri JG, Tolliver TJ, Catignani GL. Simultaneous determination of alpha-tocopherol and retinol in plasma or red cells by high pressure liquid chromatography. Am. J. Clin. Nutr. 32: 2143–2149 (1979)

    CAS  Google Scholar 

  18. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Anal. Biochem. 239: 70–76 (1996)

    Article  CAS  Google Scholar 

  19. Barja G. Free radicals and aging. Trends Neurosci. 27: 595–600 (2004)

    Article  CAS  Google Scholar 

  20. Halliwell B. Effect of diet on cancer development: Is oxidative DNA damage a biomarker? Free Radical. Bio. Med. 32: 968–974 (2002)

    Article  CAS  Google Scholar 

  21. Pratico D. Alzheimer’s disease and oxygen radicals: New insights. Biochem. Pharm. 63: 563–567 (2002)

    Article  CAS  Google Scholar 

  22. Dragsted LO. Biomarkers of exposure to vitamins A, C, and E and their relation to lipid and protein oxidation markers. Eur. J. Nutr. 4(Suppl 2): 3–18 (2008)

    Article  Google Scholar 

  23. Kang TH, Cho H, Oh H, Sohn DH, Kim YC. Flavonol glycosides with free radical-scavenging activity of Saururus chinensis. Fitoterapia 76: 115–117 (2005)

    Article  CAS  Google Scholar 

  24. Seo CS, Zheng MS, Woo MH, Lee CS, Lee SH, Jeong BS, Chang HW, Chang HW, Jahng Y, Lee ES, Son JK. Lignans from the roots of Saururus chinensis. J. Nat. Prod. 71: 1771–1774 (2008)

    Article  CAS  Google Scholar 

  25. Choi J, Lee KT, Ha J, Yun SY, Ko CD, Jung HJ, Park HJ. Antinociceptive and antiinflammatory effects of Niga-ichigoside F1 and 23-hydroxytormentic acid obtained from Rubus coreanus. Biol. Pharm. Bull. 26: 1436–1441 (2003)

    Article  CAS  Google Scholar 

  26. Lee YA, Lee MW. Tannins from Rubus coreanum. Korean J. Pharmacogn. 26: 27–30 (1995)

    CAS  Google Scholar 

  27. Pang KC, Kim MS, Lee MW. Hydrolyzable tannins from the fruits of Rubus coreanum. Korean J. Pharmacogn. 27: 366–370 (1996)

    CAS  Google Scholar 

  28. Mutlu-Turkoglu U, Ilhan E, Oztezcan S, Kuru A, Aykac-Toker G, Uysal M. Age-related increases in plasma malondialdehyde and protein carbonyl levels and lymphocyte DNA damage in elderly subjects. Clin. Biochem. 36: 397–400 (2003)

    Article  CAS  Google Scholar 

  29. Traverso N, Patriarca S, Balbis E, Furfaro AL, Cottalasso D, Pronzato MA, Carlier P, Botta F, Marinari UM, Fontana L. Anti malondialdehyde-adduct immunological response as a possible marker of successful aging. Exp. Gerontol. 38: 1129–1135 (2003)

    Article  CAS  Google Scholar 

  30. Basu S. Isoprostanes: Novel bioactive products of lipid peroxidation. Free Radical Res. 38: 105–122 (2004)

    Article  CAS  Google Scholar 

  31. Butterfield DA, Koppal T, Howard B, Subramaniam R, Hall N, Hensley K, Yatin S, Allen K, Aksenov M, Aksenova M, Carney J. Structural and functional changes in proteins induced by free radical-mediated oxidative stress and protective action of the antioxidants N-tert-butyl-alpha-phenylnitrone and vitamin E. Ann. NY Acad. Sci. 854: 448–462 (1998)

    Article  CAS  Google Scholar 

  32. Magalhaes LM, Segundo MA, Reis S, Lima JL. Methodological aspects about in vitro evaluation of antioxidant properties. Anal. Chim. Acta 613: 1–19 (2008)

    Article  CAS  Google Scholar 

  33. Loft S, Hogh Danielsen P, Mikkelsen L, Risom L, Forchhammer L, Moller P. Biomarkers of oxidative damage to DNA and repair. Biochem. Soc. T. 36: 1071–1076 (2008)

    Article  CAS  Google Scholar 

  34. Gedik CM, Grant G, Morrice PC, Wood SG, Collins AR. Effects of age and dietary restriction on oxidative DNA damage, antioxidant protection, and DNA repair in rats. Eur. J. Nutr. 44: 263–272 (2005)

    Article  CAS  Google Scholar 

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Correspondence to Mi-Kyung Sung.

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Park, SM., Choi, HY., Lee, EJ. et al. Dietary supplementation of red ginseng, Chinese lizard’s tail, and Korean raspberry extracts alleviate oxidative stresses in aged rats. Food Sci Biotechnol 19, 967–972 (2010). https://doi.org/10.1007/s10068-010-0135-6

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