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Antitumor and immunomodulating activities of endo-biopolymers obtained from a submerged culture of Pleurotus eryngii

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Abstract

Antitumor effect of endo-biopolymer (EBP) obtained from submerged mycelial culture of Pleurotus eryngii was investigated by using Sarcoma-180 bearing mice. The administration of EBP, intraperitoneally (10 to 80 mg/kg BW) at all dose ranges, inhibited the growth of the solid tumor and simultaneously increased the activity of natural killer (NK) cells significantly. While, 40 mg/kg BW treated group reduced the tumor formation (53.1%), and increased the NK cell activity of splenocytes (25.0%) to a great extent. The complement activity of the EBP was 43.1% (1,000 μg/mL). Phosphatase activity of macrophage was found to be increased up to 35.3% (100 μg/mL) when compared with control. The chemical composition of EBP was found to contain carbohydrate (77.5%) and protein (21.6%). The major component sugars of EBP were glucose and mannose, while glycine, glutamic acid, and aspartic acid mainly constituted the protein moiety. These results revealed that EBP obtained from submerged culture of P. eryngii stimulate not only immune system but also antitumor activity.

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References

  1. Suzuki I, Hashimoto K, Oikawa S, Sato K, Osawa M, Yadomae T. Antitumor and immunomodulating activities of a β-glucan obtained from liquid cultured Grifola frondosa. Chem. Pharm. Bull. 37: 410–413 (1989)

    CAS  Google Scholar 

  2. Jeong SC, Cho SP, Yang BK, Jeong YT, Ra KS, Song CH. Immunomodulating activity of the exo-polymer from submerged mycelial culture of Phellinus pini. J. Microbiol. Biotechn. 14: 15–21 (2004)

    CAS  Google Scholar 

  3. Yang BK, Kim DH, Jeong SC, Das S, Choi YS, Shin JS, Lee SC, Song CH. Hypoglycemic effect of a Lentinus edodes exo-polymer produced from a submerged mycelial culture. Biosci. Biotech. Bioch. 66: 937–942 (2002)

    Article  CAS  Google Scholar 

  4. Yang BK, Ha JY, Jeong SC, Das S, Yun JW, Lee YS, Choi JW, Song CH. Production of exo-polymers by submerged mycelial culture of Cordyceps militaris and its hypolipidemic effect. J. Microbiol. Biotechn. 10: 784–778 (2000)

    CAS  Google Scholar 

  5. Wasser SP. Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl. Microbiol. Biot. 60: 258–274 (2002)

    Article  CAS  Google Scholar 

  6. Chenghua D, Xiangliang Y, Xiaoman G, Yan W, Jingyan Z, Huibi X. A beta-d-glucan from the sclerotia of Pleurotus tuber-regium (Fr.) Sing. Carbohyd. Res. 328: 629–633 (2000)

    Article  CAS  Google Scholar 

  7. Hwang YJ, Nam HK, Chang MJ, Noh GW, Kim SH. Effect of Lentius edodes and Pleurotus eryngii extracts on proliferation and apoptosis in human colon cancer cell lines. J. Korean Soc. Food Sci. Nutr. 32: 217–222 (2003)

    Google Scholar 

  8. Kim JY, Kang HI, Park KU, Moon KD, Lee SD, Cho SH, Wee JJ, Kyung JS, Song YB, Seo KI. Antioxidative and antitumor activities of crude polysaccharide fraction from Pleurotus eryngii. J. Korean Soc. Food Sci. Nutr. 33: 1589–1593 (2004)

    Article  Google Scholar 

  9. Wang H, Gao J, Ng TB. A new lectin with highly potent antihepatoma and antisarcoma activities from the oyster mushroom Pleurotus ostreatus. Biochem. Bioph. Res. Co. 275: 810–816 (2000)

    Article  CAS  Google Scholar 

  10. Zhang M, Cheung PCK, Zhang L, Chiu CM, Ooi VEC. Carboxymethylated β-glucans from mushroom sclerotium of Pleurotus tuber-regium as novel water-soluble anti-tumor agent. Carbohyd. Polym. 57: 319–325 (2004)

    Article  CAS  Google Scholar 

  11. Zhang M, Zhang L, Cheung PCK, Ooi VEC. Molecular weight and anti-tumor activity of the water-soluble polysaccharides isolated by hot water and ultrasonic treatment from the sclerotia and mycelia of Pleurotus tuber-regium. Carbohyd. Polym. 56: 123–128 (2004)

    Article  CAS  Google Scholar 

  12. Kim KK, Kwun JY, Park YI, Bok JQ, Choi CC. Antitumor components of the cultured mycelia of Calvatia craniformis. J. Korean Cancer. Assoc. 24: 1–18 (1992)

    CAS  Google Scholar 

  13. Yang QT, Jong SC, Li XY, Zhou JX, Chen RT, Xu LZ. Antitumor and immunomodulating activities of the polysaccharopeptide (PSP) of Coriolus versicolor. J. Immunol. Immunopharmacol. 12: 29–34 (1992)

    CAS  Google Scholar 

  14. Mishell BB, Shiigi SM. Selected Methods in Cellular Immunology. 1st ed. Wh Freeman Co., San Francisco, CA, USA. pp. 4–27 (1980)

    Google Scholar 

  15. Hussain RF, Norui AME, Oliver RTD. A new approach for measurement of cytotoxicity using colorimetric assay. J. Immunol. Method 160: 89–96 (1993)

    Article  CAS  Google Scholar 

  16. Kabat EE, Mayer MM. Complement and complement fixation. pp. 133–240. In: Experimental Immunology. Charles C Thomas Publisher, Springfield, IL, USA (1964)

    Google Scholar 

  17. Suzuki H, Hyama K, Yoshida O, Yamazaki S, Yamamoto N, Toda S. Structural characterization of the immunoreactive and antiviral water-solubilized lignin in an extract of the culture medium of Lentinus edodes mycelia (LEM). Agr. Biol. Chem. Tokyo 54: 479–487 (1990)

    CAS  Google Scholar 

  18. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugar and related substance. Anal. Chem. 28: 350–356 (1956)

    Article  CAS  Google Scholar 

  19. Jones TM, Albersheim PO. A gas chromatographic method for the determination of aldose and uronic acid constituents of plant cell wall polysaccharides. Plant Physiol. 49: 926–936 (1972)

    Article  CAS  Google Scholar 

  20. Lowry OH, Rosebrough NJ, Farr L, Randall RJ. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265–275 (1951)

    CAS  Google Scholar 

  21. Duncan DB. Multiple range tests for correlated and heteroscedastic means. Biometrics 13: 164–176 (1957)

    Article  Google Scholar 

  22. Miura T, Miura NN, Ohno N, Adachi Y, Shimada S, Yadomae T. Failure in antitumor activity by overdose of an immunomodulating β-glucan preparation, sonifilan. Biol. Pharm. Bull. 23: 249–253 (2000)

    CAS  Google Scholar 

  23. Zheng R, Jie S, Hanchuan D, Moucheng W. Characterization and immunomodulating activities of polysaccharide from Lentinus edodes. Int. Immunopharmacol. 5: 811–820 (2005)

    Article  CAS  Google Scholar 

  24. Arthur MJP, Saunders PK, Wright R. Corynebacterium parvum-elicited hepatic macrophages demonstrate enhanced respiratory burst activity compared with resident Kupffer cells in the rat. Gastroenterology 91: 175–181 (1989)

    Google Scholar 

  25. Kong YY, Lee KK, Nam SY, Hong HD. Experimental studies on activity of the cultivated mycelia of Phellinus linteus. Korean J. Pharmacogn. 22: 233–239 (1991)

    Google Scholar 

  26. Moon CK, Lee SH, Mock MS, Kim DO. Antitumor activity of the polysaccharide-fraction (Copolang) from Coriolus versicolor and its effects on the immune function. Yakhak Hoeji 31: 126–132 (1987)

    CAS  Google Scholar 

  27. Herberman RB. NK cells: Their role in defense against disease. Science 214: 24–30 (1981)

    Article  CAS  Google Scholar 

  28. Pedersen BK. Natural killer cells in relation to disease and treatment. Allergy 40: 547–557 (1985)

    Article  CAS  Google Scholar 

  29. Page RC, Davies P, Allison AC. The macrophage as a secretory cell. Int. Rev. Cytol. 52: 119–123 (1978)

    Article  CAS  Google Scholar 

  30. Tsukagoshi S, Ohashi F. Protein-bound polysaccharide preparation, PS-K, effect against mouse sarcoma 180 and rat ascites hepatoma AH-13 by oral use. Gann 65: 557–558 (1974)

    CAS  Google Scholar 

  31. Kiho T, Shiose Y, Nagai K, Ukai S. Antitumor and immunomodulating activities of two polysaccharides from the fruiting bodies of Armillariella tabescens. Chem. Pharm. Bull. 40: 2110–2114 (1992)

    CAS  Google Scholar 

  32. Okuda T, Yoshioka Y, Ikekawa T, Chihara G, Nishioka K. Anticomplementary activity of anti-tumor polysaccharides. Nat. New Biol. 238: 59–60 (1972)

    Article  CAS  Google Scholar 

  33. Kim YS, Ryu KH, Mo YK, Lee CK, Han SS. Effects of the antitumor component, F-D-P, isolated from Elfvingia applanata on immune response. Korean J. Pharmacogn. 25: 348–355 (1994)

    CAS  Google Scholar 

  34. Kweon MH, Jang H, Lim WJ, Chang HI, Kim CW, Yang HC, Hwang HJ, Sung HC. Anti-complementary properties of polysaccharides isolated from fruit bodies of mushroom Pleurotus ostreatus. J. Microbiol. Biotechn. 9: 450–456 (1999)

    Google Scholar 

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Authors and Affiliations

  1. Department of Biotechnology, Daegu University, Gyeongsan, Gyeongbuk, 712-714, Korea

    Yong-Tae Jeong, Young-Ah Gu, Rezuanul Islam & Chi-Hyun Song

  2. College of Science, Technology and Environment, University of Western Sydney, Sydney, NSW, 1797, Australia

    Sang-Chul Jeong

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  1. Yong-Tae Jeong
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Correspondence to Chi-Hyun Song.

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Jeong, YT., Jeong, SC., Gu, YA. et al. Antitumor and immunomodulating activities of endo-biopolymers obtained from a submerged culture of Pleurotus eryngii . Food Sci Biotechnol 19, 399–404 (2010). https://doi.org/10.1007/s10068-010-0056-4

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