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Production and characterization of α-linolenic acid enriched structured lipids from lipase-catalyzed interesterification

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Abstract

The purpose of this study was to enrich α-linolenic acid (ALA) on the triacylglycerol (TAG) molecules of the selected vegetable oils (soybean and corn oil) in order to synthesized structural lipid (SL) with low ω6 to ω3 ratio. SLs were synthesized by lipase-catalyzed interesterification of soybean (SO) and corn oil (CO) with perilla oil (PO). Lipozyme RM IM from Rhizomucor miehei (5% by weight of total substrate) was used for the reaction. The reaction was performed during 24 hr at 55°C in the stirred-batch type reactor with solvent free system. After 24 hr reaction the ratio of ω6 to ω3 in SL products dramatically decreased comparing to that of substrates (SO and CO) before the reaction. At sn-2 position, the contents of ALA were 25.99% in soybean-SL and 23.25% in corn-SL, respectively. According to the equivalent carbon number (ECN) of TAG profile, newly produced SLs were separated by reverse-phase high performance liquid chromatography (HPLC). These results indicated that lipase-catalyzed interesterification is effective to produce SL with low ω6 to ω3 ratio which might be used as health beneficial oil.

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Authors and Affiliations

  1. Department of Food Science and Technology, Chungnam National University, Daejeon, 305-764, Korea

    Jeung-Hee Lee & Ki-Teak Lee

  2. Department of Food and Nutrition, Chungnam National University, Daejeon, 305-764, Korea

    Kanika Mitra & Seong-Ai Kim

  3. Department of Food and Nutrition, Catholic University of Daegu, Gyeongsan, Gyeongbuk, 712-702, Korea

    Sang-Won Choi

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  1. Kanika Mitra
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  2. Seong-Ai Kim
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  3. Jeung-Hee Lee
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  5. Ki-Teak Lee
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Correspondence to Ki-Teak Lee.

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Mitra, K., Kim, SA., Lee, JH. et al. Production and characterization of α-linolenic acid enriched structured lipids from lipase-catalyzed interesterification. Food Sci Biotechnol 19, 57–62 (2010). https://doi.org/10.1007/s10068-010-0008-z

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  • DOI: https://doi.org/10.1007/s10068-010-0008-z

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