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Genetic polymorphisms of IL-27 and risk of systemic lupus erythematosus disease in the Egyptian population

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Abstract

Objective

Cytokine polymorphisms have been associated with systemic lupus erythematosus (SLE) pathogenicity. Interleukin 27 (IL-27) is an important one of pro-/anti-inflammatory cytokine. It has been reported in various Th1/Th17-mediated inflammatory disorders, and even in Th2-complexed diseases, such as SLE. In our preliminary study, the aim was to investigate the potential roles of single nucleotide polymorphism (SNP) -964A/G (rs153109) and + 2905 T/G (rs17855750) in an IL-27p28 gene on susceptibility to SLE.

Methods

The 112 Egyptian SLE patients against 101 healthy persons were enrolled in this work. The polymerase chain reaction/restriction fragment length polymorphism (PCR–RFLP) is used for genotyping IL-27 SNPs.

Results

No significant variations were found between patients and control in the genotype and allele frequencies of IL-27p28 (-964A/G). SLE patients have a significant increase in the frequency of IL-27p28 (+ 2905 T/G) TG genotype (P < 0.01) and G allele (P < 0.01) compared to controls. Complete disappearance of GG genotype was demonstrated in both groups. G allele might have considered a disease risk factor with odd ration (OR) = 9.184. From four possible haplotypes, the frequency of AT haplotype elevated in both examined groups.

Conclusion

This was the first study on the Egyptian population for studying the relation between IL-27 SNPs and SLE. Our preliminary study indicated that both TG genotype and G allele of IL-27p28 (+ 2905 T/G) could consider risk factors for SLE.

Key Points

• This article provides an information about the relation between systemic lupus erythematosus and interleukin-27 cytokine by detection single nucleotide polymorphism.

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Acknowledgements

The authors would like to express their appreciation to Prof. Dr. Roba Mohamed Talaat, Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), Sadat City University, Egypt, for her unlimited help throughout the work. We also would thank the nursing staff of the Rheumatology and Rehabilitation Department, Faculty of Medicine, Cairo University, Cairo, Egypt.

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Authors and Affiliations

  1. Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City , Sadat City, Egypt

    Yasser B. M. Ali & Basima A. El-Akhras

  2. Rheumatology and Rehabilitation Department, Faculty of Medicine, Cairo University, Cairo, Egypt

    R. El-Shazly & Iman H. Bassyouni

Authors
  1. Yasser B. M. Ali
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  2. Basima A. El-Akhras
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  3. R. El-Shazly
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  4. Iman H. Bassyouni
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Contributions

YA conceived and designed the study. Re and IH collected the samples and prepared all clinical sheets. They provided expert guidance on SLE manifestations. BA performed experiments. YA and BA analyzed and interpreted the data. BA wrote the manuscript. YA revised the manuscript.

Corresponding author

Correspondence to Yasser B. M. Ali.

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Ethics committee approval

Ethical Committee at Kasr El-Aini hospital approved this study and reported agreement was obtained from all cases.

Informed consent

At the beginning of this work, all patients were informed and their consent was taken while recording all diagnostic data for each case.

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None.

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Ali, Y.B.M., El-Akhras, B.A., El-Shazly, R. et al. Genetic polymorphisms of IL-27 and risk of systemic lupus erythematosus disease in the Egyptian population . Clin Rheumatol 40, 4899–4907 (2021). https://doi.org/10.1007/s10067-021-05858-6

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  • DOI: https://doi.org/10.1007/s10067-021-05858-6

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