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Clinical features and expression of type I interferon-inducible genes in systemic lupus erythematosus patients harboring rs1143679 polymorphism in China: a single-center, retrospective study

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Abstract

Objective

This case-control study aimed to analyze the clinical features and determine the expression of type I interferon-induced genes in systemic lupus erythematosus (SLE) patients harboring the CD11b rs1143679 single-nucleotide polymorphism (SNP) and elucidate whether it is involved in the relapses of SLE.

Methods

One hundred twenty-five relatively inactive SLE patients with SLEDAI scores < 6, including 102 CD11b rs1143679 G allele patients as controls and 23 rs1143679 A allele carriers as cases, were enrolled from the SLE patient specimen bank in the Department of Rheumatology and Immunology. The sample set was retrospectively analyzed for differences in clinical features, and quantitative PCR and Western blot analyses were performed to evaluate the relative expression of type I interferon (IFN)-inducible genes.

Results

The 24-h urinary protein levels in the case group were significantly elevated, and serum C3 levels were significantly reduced compared with those in the control group (P = 0.019 and P = 0.021, respectively). The relative mRNA levels of IFN-inducible genes IFIT1, IFIT4, and ISG15 in the case group were higher than that in the control group (P = 0.0257, 0.0344, and 0.0311, respectively) and matched with the Western blot results.

Conclusions

The relative expression of type I IFN-inducible genes in inactive SLE patients harboring the CD11b rs1143679 polymorphism was higher than that in other lupus patients. These findings suggest that the rs1143679 SNP can precipitate relapses in inactive SLE patients.

Key Points

• The rs1143679 GA genotype was associated with SLE clinical features.

• The rs1143679 GA genotype showed higher interferon-inducible gene expression relative to the GG genotype.

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Funding

This work was supported by the Key Programs of Medical Science and Technology Development Foundation of the Nanjing Department of Health (Grant #QRX11026).

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Author notes

  1. Wenyu Xu, Yueyue Zhang and Xiaoqin Wang contributed equally to this work.

Authors and Affiliations

  1. Department of Rheumatology and Immunology, Nanjing First Hospital, Nanjing Medical University, No. 68 Changle Road, Nanjing, 210006, China

    Wenyu Xu, Yueyue Zhang, Xiaoqin Wang, Dayu Gao, Bingjie Gu, Junyu Zhang, Chunmei Li, Qijie Ren, Leilei Yang, Hai Yuan, Minning Shen & Xingguo Chen

  2. Department of Pharmacology, School of Basic Medical Science, Nanjing Medical University, Nanjing, 211166, China

    Peiyu Liu

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  1. Wenyu Xu
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Contributions

All authors contributed to the study conception and design. Bingjie Gu, Qijie Ren, Leilei Yang, Hai Yuan, and Minning Shen provided the patient samples. Material preparation, data collection, and analysis were performed by Wenyu Xu, Yueyue Zhang, and Xiaoqin Wang. The first draft of the manuscript was written by Wenyu Xu and Xingguo Chen. Peiyu Liu, Dayu Gao, Junyu Zhang, and Chunmei Li commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Xingguo Chen.

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The protocol for the study was approved by the Medical Ethics Committee of Nanjing Medical University (Nanjing, China).

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Xu, W., Zhang, Y., Wang, X. et al. Clinical features and expression of type I interferon-inducible genes in systemic lupus erythematosus patients harboring rs1143679 polymorphism in China: a single-center, retrospective study. Clin Rheumatol 40, 1093–1101 (2021). https://doi.org/10.1007/s10067-020-05330-x

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  • DOI: https://doi.org/10.1007/s10067-020-05330-x

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