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JAK2 and PTPRC mRNA expression in peripheral blood mononuclear cells from patients with systemic lupus erythematosus

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Abstract

In this study, we aimed to explore the expression levels of JAK2 and PTPRC in peripheral blood mononuclear cells (PBMCs) from SLE patients and controls, detect the effects of SLE activity on genes mRNA expression, and find the association between genes mRNA expression and clinical manifestations of patients. We performed quantitative real-time PCR (qRT-PCR) to test differences in the expression levels of JAK2 and PTPRC in PBMCs extracted from 135 patients with SLE and 130 healthy controls. Furthermore, we detected the regulatory effect of SNPs on gene expression by expression quantitative trait loci (eQTL). We also tested whether the genes mRNA expression was affected with the SLE activity and analyzed the relationship between genes mRNA expression and clinical manifestations of patients. The mRNA expression levels of JAK2 in SLE patients were significantly higher than those in healthy controls (P = 0.005), and PTPRC mRNA expression levels were significantly decreased (P < 0.001). However, no other statistical significance was detected. We found that the elevated JAK2 mRNA expression and the decreased PTPRC mRNA expression may play suggestive roles in the pathogenesis of SLE.

Key Points

• The JAK2 mRNA expression levels in SLE patients were significantly higher than those in healthy controls.

• The PTPRC mRNA expression levels in SLE were decreased.

• JAK2 and PTPRC mRNA expression may play suggestive roles in the pathogenesis of SLE.

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Acknowledgments

We thank all the study participants and all the volunteers who have so willingly participated in this study, thus making this study possible.

Funding

This study was supported financially by the National Key Research and Development Program of China (2017YFC0909001 and 2016YFC0906102), the National Natural Science Foundation of China (81872527, 81803117, and 8132018016) and the Natural Science Foundation of Anhui Province (1808085QH284).

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Author notes

  1. Danfeng Qian, Lu Liu and Tingting Zhu contributed equally to this work.

Authors and Affiliations

  1. Institute of Dermatology and Department of Dermatology, The First Affiliated Hospital, Anhui Medical University, 81 Meishan Road, Shushan District, Hefei, 230032, Anhui, China

    Danfeng Qian, Lu Liu, Tingting Zhu, Leilei Wen, Zhengwei Zhu, Mingshun Wu, Xueying Li, Jie Ma, Qun Zhang, Ling Jin, Xiaomeng Wang, Qiaohu Xu, Sen Yang, Yujun Sheng & Xuejun Zhang

  2. Department of Biochemistry and Molecular Biology, Anhui Medical University, 81 Meishan Road, Shushan District, Hefei, Auhui, China

    Danfeng Qian, Tingting Zhu & Shengquan Zhang

  3. Center for Statistical Genetics, Department of Biostatistics, University of Michigan, Ann Arbor, MI, 48109, USA

    Xianyong Yin

  4. Department of Dermatology, Jining No. 1 People’s Hospital, Shandong, People’s Republic of China

    Ying Qiu

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  1. Danfeng Qian
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  2. Lu Liu
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Correspondence to Yujun Sheng, Shengquan Zhang or Xuejun Zhang.

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Qian, D., Liu, L., Zhu, T. et al. JAK2 and PTPRC mRNA expression in peripheral blood mononuclear cells from patients with systemic lupus erythematosus. Clin Rheumatol 39, 443–448 (2020). https://doi.org/10.1007/s10067-019-04778-w

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  • DOI: https://doi.org/10.1007/s10067-019-04778-w

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