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Influence of different supplementation on platelet aggregation in patients with rheumatoid arthritis

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Abstract

Introduction

Long-chain n-3 polyunsaturated fatty acids (n-3 PUFAs; eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) have been reported to reduce platelet aggregation. Our aim was to prospectively assess the potential influence of different supplementation omega-3 PUFA on the antiplatelet effects in rheumatoid arthritis (RA) patients.

Methods

The study included 60 patients with RA at the Department of Rheumatology, Clinical Center Kragujevac. Patients were divided into three groups depending on who used concentrated fish oil only or concentrated fish oil in combination with evening primrose oil or control group without supplementation in a period of 3 months. Platelet aggregation was measured using the multiplate analyzer and expressed through the value of adenosine diphosphate (ADP) test, aranchidonic acid–induced aggregation (ASPI) test, thrombin receptor–activating peptide (TRAP) test (to assess baseline platelet aggregation), and the ratio of ADP/TRAP and ASPI/TRAP representing the degree of inhibition of platelet aggregation compared to the basal value. The platelet function analysis in whole blood was performed 18–24 h before starting supplementation and after 90 days. Considerations were taken in the representation of demographic, clinical characteristics, and laboratory parameters between the groups.

Results

Patients who used concentrated fish oil only had a significantly lower value of the ratio of ADP/TRAP (0.68 ± 0.20) compared to patients without supplementation (0.83 ± 0.12; p = 0.008), while there was no statistically significant difference in values of other laboratory parameters of platelet function between other groups.

Conclusions

Co-administration of supplementation-concentrated fish oil may reduce platelet aggregation in adults with RA.

Key Points

• Omega-3 PUFAs are essential for health and are known to possess anti-inflammatory properties, improving cardiovascular health as well as benefiting inflammatory diseases..

• In this paper, we report on anti-aggregation effects n-3 PUFAs and ɤ-linolenic acid in RA.

• The risk of cardiovascular morbidity and mortality is increased in RA, and dietary supplementation of n-3 PUFA may have preventive potential for the cardiovascular management in rheumatoid arthritis.

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Authors and Affiliations

  1. Public Health Center, DZ Rakovica, 22 Kraljice Jelene, 11000, Belgrade, Serbia

    Marijana Tomic-Smiljanic

  2. Faculty of Medical Sciences, Department of Hygiene and Ecology, University of Kragujevac, 69 Svetozara Markovica, 34000, Kragujevac, Serbia

    Dragan Vasiljevic

  3. Faculty of Medical Sciences, Department of Internal medicine, University of Kragujevac, 69 Svetozara Markovica, 34000, Kragujevac, Serbia

    Aleksandra Lucic-Tomic, Nebojsa Andjelkovic & Mirjana Veselinovic

  4. Faculty of Medical Sciences, Department of Physiology, University of Kragujevac, 69 Svetozara Markovica, 34000, Kragujevac, Serbia

    Vladimir Jakovljevic

  5. Department of Human Pathology, 1st Moscow State Medical, University IM Sechenov, Trubetskaya Street 8, Str. 2, Moscow, Russia, 119991

    Vladimir Jakovljevic & Sergey Bolovich

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  1. Marijana Tomic-Smiljanic
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Correspondence to Mirjana Veselinovic.

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Tomic-Smiljanic, M., Vasiljevic, D., Lucic-Tomic, A. et al. Influence of different supplementation on platelet aggregation in patients with rheumatoid arthritis. Clin Rheumatol 38, 2443–2450 (2019). https://doi.org/10.1007/s10067-019-04569-3

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  • DOI: https://doi.org/10.1007/s10067-019-04569-3

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