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Evaluation of the effect of polyphenol of escin compared with ibuprofen and dexamethasone in synoviocyte model for osteoarthritis: an in vitro study

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Abstract

Osteoarthritis (OA) is a chronic degenerative joint disease with inflammatory component. It is associated with progressive histological alterations and disabling symptoms. Today, drugs such as glucocorticoids (GCs) and nonsteroidal anti-inflammatory drugs (NSIADs) are commonly employed for treatment of osteoarthritis, but have serious and life-threatening side effects. The aim of the current study is to evaluate the effects of escin on cyclooxygenase-2 (COX-2, isoform), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), interleukin-18 (IL-18), tumor necrosis factor-alpha (TNF-α), and nitric oxide (NO) (1), as well as prostaglandin E2 (PGE2) on inflammatory cells, similar osteoarthritis in synoviocytes, and monocytes/macrophages, and to compare it with dexamethasone (DEX) and ibuprofen (IBP). Synovial cells were isolated from synovial membrane of the radiocarpal joint cartilage of an 8-month-old Holstein cow. THP-1 cells were prepared from Pasteur Institute of Iran. Cells were cultivated and exposed to lipopolysaccharide (LPS) stimulation without, or in the presence of, DEX, IBP, or escin. The gene expressions of IL-1β, TNF-α, IL-18, COX-2, and iNOS were evaluated by real-time PCR. Concentrations of NO and PGE2 were measured by ELISA methods. Our cells secreted an increased amounts of IL-1β, TNF-α, IL-18, COX-2, iNOS, NO, and PGE2 in response to LPS stimulation in all conditions. Escin can quench the gene expression of COX-2, iNOS, IL-1β, IL-18, and TNF-α in synoviocyte cells and production of NO and PGE2 in monocyte/macrophage cells alike DEX and IBP. We can use from escin for the treatment of osteoarthritis as an anti-inflammatory agent in the latter but further studies to support the results from such a model are needed.

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References

  1. Lane NE, Shidara K, Wise BL (2017) Osteoarthritis year in review 2016: clinical. Osteoarthr Cartil 25(2):209–215. https://doi.org/10.1016/j.joca.2016.09.025

    Article  PubMed  CAS  Google Scholar 

  2. Blaney Davidson EN, van Caam AP, van der Kraan PM (2017) Osteoarthritis year in review 2016: biology. Osteoarthr Cartil 25(2):175–180. https://doi.org/10.1016/j.joca.2016.09.024

    Article  PubMed  CAS  Google Scholar 

  3. Heidari B (2011) Knee osteoarthritis prevalence, risk factors, pathogenesis and features: part I. Caspian J Int Med 2(2):205–212

    Google Scholar 

  4. Felson DT, Zhang Y, Hannan MT, Naimark A, Weissman BN, Aliabadi P, Levy D (1995) The incidence and natural history of knee osteoarthritis in the elderly, the framingham osteoarthritis study. Arthritis Rheumatol 38(10):1500–1505

    Article  CAS  Google Scholar 

  5. Srikanth VK, Fryer JL, Zhai G, Winzenberg TM, Hosmer D, Jones G (2005) A meta-analysis of sex differences prevalence, incidence and severity of osteoarthritis. Osteoarthr Cartil 13(9):769–781

    Article  PubMed  Google Scholar 

  6. Muraki S, Akune T, Oka H, Mabuchi A, En-Yo Y, Yoshida M, Saika A, Nakamura K, Kawaguchi H, Yoshimura N (2009) Association of occupational activity with radiographic knee osteoarthritis and lumbar spondylosis in elderly patients of population-based cohorts: a large-scale population-based study. Arthritis Rheum 61(6):779–786

    Article  PubMed  Google Scholar 

  7. Nelson AE, Braga L, Renner JB, Atashili J, Woodard J, Hochberg MC, Helmick CG, Jordan JM (2010) Characterization of individual radiographic features of hip osteoarthritis in African American and White women and men: the Johnston County Osteoarthritis Project. Arthritis Care Res 62(2):190–197

    Google Scholar 

  8. Fernandes JC, Martel-Pelletier J, Pelletier JP (2002) The role of cytokines in osteoarthritis pathophysiology. Biorheology 39(1–2):237–246

    PubMed  CAS  Google Scholar 

  9. Okada Y, Nakanishi I, Kajikawa K (1981) Ultrastructure of the mouse synovial membrane. Development and organization of the extracellular matrix. Arthritis Rheum 24(6):835–843

    Article  PubMed  CAS  Google Scholar 

  10. Palmer RM, Hickery MS, Charles IG, Moncada S, Bayliss MT (1993) Induction of nitric oxide synthase in human chondrocytes. Biochem Biophys Res Commun 193(1):398–405

    Article  PubMed  CAS  Google Scholar 

  11. Geng Y, Blanco FJ, Cornelisson M, Lotz M (1995) Regulation of cyclooxygenase-2 expression in normal human articular chondrocytes. J Immunol 155(2):796–801

    PubMed  CAS  Google Scholar 

  12. Day RO, Graham GG (2013) Non-steroidal anti-inflammatory drugs (NSAIDs). BMJ (Clin Res Ed) 346:f3195. https://doi.org/10.1136/bmj.f3195

    Article  Google Scholar 

  13. Behrens F, Shepard N, Mitchell N (1975) Alterations of rabbit articular cartilage by intra-articular injections of glucocorticoids. J Bone Joint Surg Am 57(1):70–76

    Article  PubMed  CAS  Google Scholar 

  14. Nakamura M, Watanabe J, Ogawa R, Kanamura S (1997) Immunohistochemical localization of type II and type I collagens in articular cartilage of the femoral head of dexamethasone-treated rats. Histochem J 29(9):645–654

    Article  PubMed  CAS  Google Scholar 

  15. Singh H, Sidhu S, Chopra K, Khan MU (2017) The novel role of beta-aescin in attenuating CCl4-induced hepatotoxicity in rats. Pharm Biol 55(1):749–757. https://doi.org/10.1080/13880209.2016.1275023

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  16. Xiao GM, Wei J (2005) Effects of beta-Aescin on the expression of nuclear factor-kappaB and tumor necrosis factor-alpha after traumatic brain injury in rats. J Zhejiang Univ Sci B 6(1):28–32. https://doi.org/10.1631/jzus.2005.B0028

    Article  PubMed  CAS  Google Scholar 

  17. Wang H, Zhang L, Jiang N, Wang Z, Chong Y, Fu F (2013) Anti-inflammatory effects of escin are correlated with the glucocorticoid receptor/NF-kappaB signaling pathway, but not the COX/PGF2alpha signaling pathway. Exp Ther Med 6(2):419–422. https://doi.org/10.3892/etm.2013.1128

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  18. Xin W, Zhang L, Sun F, Jiang N, Fan H, Wang T, Li Z, He J, Fu F (2011) Escin exerts synergistic anti-inflammatory effects with low doses of glucocorticoids in vivo and in vitro. Phytomedicine 18(4):272–277. https://doi.org/10.1016/j.phymed.2010.08.013

    Article  PubMed  CAS  Google Scholar 

  19. Du Y, Song Y, Zhang L, Zhang M, Fu F (2016) Combined treatment with low dose prednisone and escin improves the anti-arthritic effect in experimental arthritis. Int Immunopharmacol 31:257–265. https://doi.org/10.1016/j.intimp.2016.01.006

    Article  PubMed  CAS  Google Scholar 

  20. Dijkstra G, Moshage H, van Dullemen HM, de Jager-Krikken A, Tiebosch AT, Kleibeuker JH, Jansen PL, van Goor H (1998) Expression of nitric oxide synthases and formation of nitrotyrosine and reactive oxygen species in inflammatory bowel disease. J Pathol 186(4):416–421. https://doi.org/10.1002/(sici)1096-9896(199812)186:4<416::aid-path201>3.0.co;2-u

    Article  PubMed  CAS  Google Scholar 

  21. Dubois RN, Abramson SB, Crofford L, Gupta RA, Simon LS, Van De Putte LB, Lipsky PE (1998) Cyclooxygenase in biology and disease. FASEB J 12(12):1063–1073

    Article  PubMed  CAS  Google Scholar 

  22. Luzzi R, Feragalli B, Belcaro G, Cesarone MR, Cornelli U, Dugall M, Hosoi M (2011) Aescin: microcirculatory activity. Effects of accessory components on clinical and microcirculatory efficacy. Panminerva Med 53(3 Suppl 1):51–55

    PubMed  CAS  Google Scholar 

  23. Lanas A, Garcia-Tell G, Armada B, Oteo-Alvaro A (2011) Prescription patterns and appropriateness of NSAID therapy according to gastrointestinal risk and cardiovascular history in patients with diagnoses of osteoarthritis. BMC Med 9:38. https://doi.org/10.1186/1741-7015-9-38

    Article  PubMed  PubMed Central  Google Scholar 

  24. Bresalier RS, Sandler RS, Quan H, Bolognese JA, Oxenius B, Horgan K, Lines C, Riddell R, Morton D, Lanas A, Konstam MA, Baron JA (2005) Cardiovascular events associated with rofecoxib in a colorectal adenoma chemoprevention trial. N Engl J Med 352(11):1092–1102. https://doi.org/10.1056/NEJMoa050493

    Article  PubMed  CAS  Google Scholar 

  25. Raetz CR, Whitfield C (2002) Lipopolysaccharide endotoxins. Annu Rev Biochem 71:635–700. https://doi.org/10.1146/annurev.biochem.71.110601.135414

    Article  PubMed  CAS  Google Scholar 

  26. Kuo PC, Schroeder RA (1995) The emerging multifaceted roles of nitric oxide. Ann Surg 221(3):220–235

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  27. Kost SAH, Roner LA, Gray JG (1995) A cyclic adenosine 3,5-monophosphate signal is required for the induction of IL-1b by TNF-a in human monocytes. J Immunol 155:836–844

    PubMed  Google Scholar 

  28. Ferrero-Miliani L, Nielsen OH, Andersen PS, Girardin SE (2007) Chronic inflammation: importance of NOD2 and NALP3 in interleukin-1beta generation. Clin Exp Immunol 147(2):227–235. https://doi.org/10.1111/j.1365-2249.2006.03261.x

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  29. Wu MJ, Wang L, Ding HY, Weng CY, Yen JH (2004) Glossogyne tenuifolia acts to inhibit inflammatory mediator production in a macrophage cell line by downregulating LPS-induced NF-kappa B. J Biomed Sci 11(2):186–199. https://doi.org/10.1159/000076031

    Article  PubMed  CAS  Google Scholar 

  30. Olee T, Hashimoto S, Quach J, Lotz M (1999) IL-18 is produced by articular chondrocytes and induces proinflammatory and catabolic responses. J Immunol 162(2):1096–1100

    PubMed  CAS  Google Scholar 

  31. Futani H, Okayama A, Matsui K, Kashiwamura S, Sasaki T, Hada T, Nakanishi K, Tateishi H, Maruo S, Okamura H (2002) Relation between interleukin-18 and PGE2 in synovial fluid of osteoarthritis: a potential therapeutic target of cartilage degradation. J Immunother 25(1):S61–S64

    Article  PubMed  CAS  Google Scholar 

  32. Li Y, Jiang JM, Yang DH, Wang FL, Mao ZX (2009) Determination of the concentrations of interleukin-18 and other cytokines in the synovial fluid in patients with osteoarthritis. Nan Fang Yi Ke Da Xue Xue Bao 29(4):729–731

    PubMed  CAS  Google Scholar 

  33. Wang FL, Jiang JM, Wang F, Fu ZZ, Zhang ZF (2010) Expressions of interleukin 18 and prostaglandin E2 and their correlation in the synoviocytes of patients with osteoarthritis. Nan Fang Yi Ke Da Xue Xue Bao 30(4):731–733

    PubMed  CAS  Google Scholar 

  34. Cho ML, Jung YO, Moon YM, Min SY, Yoon CH, Lee SH, Park SH, Cho CS, Jue DM, Kim HY (2006) Interleukin-18 induces the production of vascular endothelial growth factor (VEGF) in rheumatoid arthritis synovial fibroblasts via AP-1-dependent pathways. Immunol Lett 103(2):159–166

    Article  PubMed  CAS  Google Scholar 

  35. de Lange-Brokaar BJ, Ioan-Facsinay A, van Osch GJ, Zuurmond AM, Schoones J, Toes RE, Huizinga TW, Kloppenburg M (2012) Synovial inflammation, immune cells and their cytokines in osteoarthritis: a review. Osteoarthritis Cartilage/OARS, Osteoarthritis Res Soc 20(12):1484–1499

    Article  Google Scholar 

  36. Farahat MN, Yanni G, Poston R, Panayi GS (1993) Cytokine expression in synovial membranes of patients with rheumatoid arthritis and osteoarthritis. Ann Rheum Dis 52(12):870–875

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  37. Massicotte F, Lajeunesse D, Benderdour M, Pelletier JP, Hilal G, Duval N, Martel-Pelletier J (2002) Can altered production of interleukin-1beta, interleukin-6, transforming growth factor-beta and prostaglandin E(2) by isolated human subchondral osteoblasts identify two subgroups of osteoarthritic patients. Osteoarthritis Cartilage/OARS, Osteoarthritis Res Soc 10(6):491–500

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biology, Payame Noor University Tehran, Tehran, Iran

    Hossein Maghsoudi

  2. Department of Clinical Biochemistry, Maraghe University of Medical Science, Maraghe, Iran

    Jamal Hallajzadeh

  3. Rheumatology Research Center, Firoozgar Hospital, Iran University of Medical Science, Tehran, Iran

    Mozhghan Rezaeipour

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  1. Hossein Maghsoudi
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  2. Jamal Hallajzadeh
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  3. Mozhghan Rezaeipour
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Correspondence to Jamal Hallajzadeh.

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Maghsoudi, H., Hallajzadeh, J. & Rezaeipour, M. Evaluation of the effect of polyphenol of escin compared with ibuprofen and dexamethasone in synoviocyte model for osteoarthritis: an in vitro study. Clin Rheumatol 37, 2471–2478 (2018). https://doi.org/10.1007/s10067-018-4097-z

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  • DOI: https://doi.org/10.1007/s10067-018-4097-z

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