ADAM-17 is expressed in the inflammatory myopathy and is involved with interstitial lung disease
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The “A disintegrin and metalloprotease” (ADAM) family is thought to play an important role in tissue destruction and inflammatory reactions. ADAM-17 was first described as the protease responsible for tumor necrosis factor (TNF)-α shedding. Here, we have shown the expression of ADAM-17 in inflammatory myopathy and demonstrated the role of inflammation in interstitial lung diseases (ILD). ADAM-17 in inflammatory myopathy serum [polymyositis (n = 26), dermatomyositis (n = 34), and clinically amyopathic dermatomyositis (n = 10)] and healthy control (n = 19) was measured using enzyme-linked immunosorbent assay. The relationship between ADAM-17 and clinical data was examined. Finally, we performed immunohistological analysis to investigate the expression of ADAM-17 on the muscles of the inflammatory myopathy patients. ADAM-17 in inflammatory myopathy was significantly higher than that in healthy control (mean ± SEM, 1048 ± 312 and 36 ± 18 pg/ml, respectively; p < 0.05). ADAM-17 in post-treatment with corticosteroid and/or immunosuppressant serum was significantly decreased compared with that in pre-treatment serum (1465 ± 562 and 1059 ± 503 pg/ml, respectively; p < 0.01). ADAM-17 was significantly positively correlated with fractalkine/CX3CL1 and CXCL16. In addition, ADAM-17 in inflammatory myopathy with ILD patients (n = 46) was significantly higher than that in non-ILD patients (n = 24) (1379 ± 454 and 413 ± 226 pg/ml, respectively; p < 0.05). We found the expression of ADAM-17 on muscle biopsy tissue. ADAM-17 is expressed in inflammatory myopathies especially ILD, suggesting that ADAM-17 plays a role in lung fibrosis. ADAM-17 may be a potential target in inflammatory myopathies with ILD.
KeywordsADAM-17 CXCL16 Fractalkine/CX3CL1 Inflammatory myopathy Interstitial lung disease
interstitial lung disease
A disintegrin and metalloprotease
tumor necrosis factor
clinically amyopathic dermatomyositis
experimental autoimmune myositis
enzyme-linked immunosorbent assay
fetal bovine serum
phosphate buffered saline
human lung fibroblasts
transforming growth factor
major histocompatibility complex I
interferon gamma-induced protein-10/CXCL10
We thank Ms. Takeuchi for performing all ELISAs.
AN performed all assays with assistance from TI, SN, SI, TT, HF, and KW. TI also assisted with the acquisition of data. AN performed the statistical analysis. TI, KW, and TK conceived the study and participated in its design and coordination. TI assisted AN with drafting the manuscript. All authors read and approved the final manuscript.
Compliance with ethical standards
We obtained written informed consent from all patients who enrolled in the study. The study received approval from the Bio-Ethics Committee of the Department of Medicine, Showa University School of Medicine (No. 1892).
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