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Relative abundance of Megamonas hypermegale and Butyrivibrio species decreased in the intestine and its possible association with the T cell aberration by metabolite alteration in patients with Behcet’s disease (210 characters)

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Abstract

Objectives

We have previously demonstrated that the phylum Actinobacteria, the family Lactobacillaceae, and the genus Bifidobacterium increased in relative abundance of gut microbiota in patients with Behcet’s disease (BD). The phylum Firmicutes and the class Clostridia were predominant in the feces of normal individuals. The class Clostridia includes short-chain fatty acid–producing bacteria, important for the balance between regulatory T cells and helper T type 17 (Th17) cells. It is possible that the bacterial compositional alteration causes low intestinal short-chain fatty acid concentrations, leading to skewed immune functions in patients with BD.

Methods

To test the hypothesis, we examined species composition and gene functions from the 16S rRNA data by utilizing PICRUSt software.

Results

We have shown that relative abundance of Eggerthella lenta, Acidaminococcus species, Lactobacillus mucosae, Bifidobacterium bifidum, Lactobacillus iners, Streptococcus species, and Lactobacillus salivarius increased significantly in patients with BD. Relative abundance of Megamonas hypermegale, Butyrivibrio species, Streptococcus infantis, and Filifactor species increased significantly in normal individuals compared with BD patients. In the functional annotation analysis by PICRUSt, we found prevalent gene functions of the pentose phosphate pathway and the inosine monophosphate biosynthesis in patients with BD. The data suggested that BD gut microbes altered nucleic acid and fatty acid synthesis.

Conclusions

These compositional and functional alterations of gut microbes may accompany unfavorable molecular exchanges between intestinal immunocompetent cells and gut microbes, and these interactions may have an association with the immune aberration in patients with BD.

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Acknowledgements

The authors are grateful to Dr. Masanori A. Murayama (Department of Immunology and Medicine, St. Marianna University School of Medicine) for his help in the review process.

Funding

This study was supported in part by Grants-in-Aid for Scientific Research (Grant number: 17K10009) of Japan Society for the Promotion of Science.

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Authors and Affiliations

  1. Department of Immunology and Medicine, St. Marianna University School of Medicine, Sugao 2-16-1, Miyamae-ku, Kawasaki, 216-8511, Japan

    Jun Shimizu, Takao Kubota, Erika Takada, Kenji Takai, Naruyoshi Fujiwara, Nagisa Arimitsu, Yuji Ueda, Sueshige Wakisaka, Tomoko Suzuki & Noboru Suzuki

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  1. Jun Shimizu
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  2. Takao Kubota
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Correspondence to Noboru Suzuki.

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This study was approved by the institutional review boards of St. Marianna University School of Medicine and was registered with the University Hospital Medical Information Network-Clinical Trials Registry (UMIN000018937). We conducted this research according to the principles expressed in the Declaration of Helsinki. We obtained written informed consent from each individual prior to enrolment in the study. A copy of the written consent is available for review upon request.

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Shimizu, J., Kubota, T., Takada, E. et al. Relative abundance of Megamonas hypermegale and Butyrivibrio species decreased in the intestine and its possible association with the T cell aberration by metabolite alteration in patients with Behcet’s disease (210 characters). Clin Rheumatol 38, 1437–1445 (2019). https://doi.org/10.1007/s10067-018-04419-8

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