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Clinical Rheumatology

, Volume 37, Issue 3, pp 639–646 | Cite as

Changes and clinical significance of CD8+CD122+ T cells in the peripheral blood of patients with ankylosing spondylitis

  • Renfang Han
  • Xiao Yang
  • Mengya Chen
  • Xu Zhang
  • Yaping Yuan
  • Xingxing Hu
  • Mengmeng Wang
  • Rui Liu
  • Yubo Ma
  • Jiajia Yang
  • Shengqian Xu
  • Zongwen Shuai
  • Shanqun Jiang
  • Faming Pan
Original Article

Abstract

The aim of our study was to explore the relationship between circulating T cells and ankylosing spondylitis (AS) and to find the role of the CD8+CD122+ T cells in the pathogenesis and progression of AS. With the method of case-control design, flow cytometry was performed to quantitatively determine the percentage of circulating CD8+CD122+ T cells in peripheral blood mononuclear cells from established AS patients and age- and gender-matched healthy controls. Serum levels of inflammatory cytokines like interleukin-2, interleukin-10, interleukin-15, TGF-β1, and TNF-ɑ were detected by enzyme-linked immunosorbent assay. The t test was used to compare differences between groups, correlation analysis with Spearman rank test and Pearson correlation was performed. The percentage of circulating CD8+CD122+ T cells were significantly increased in AS patients compared with controls (Z = − 4.917, P = 0.001). Plasma IL-2, IL-10, IL-15, TGF-β1, and TNF-ɑ levels between cases and controls were analyzed but no statistically significant differences were found. The percentage of circulating CD8+CD122+ T cells were not significantly different in the two groups of patients with AS whether they were treated or not. In addition, the percentage of circulating CD8+CD122+ T cells was positively correlated with disease duration, CRP, and ASDAS-CRP. The CD8+CD122+ T cells in the peripheral blood of AS patients may be involved in the development of AS, and they may coordinate regulate inflammation and immune dysregulation in patients with AS, which may play an important role in the pathogenesis and prognosis of AS.

Keywords

Ankylosing spondylitis CD8+CD122+ Cytokine Regulatory T cells 

Notes

Acknowledgements

We thank all the patients and blood donors who participated in our study.

Funding information

This study was supported by grants from the National Natural Science Foundation of China (30771849, 30972530, 81273169, 81573218 and 81773514).

Compliance with ethical standards

The study was approved by the Local Ethics Research Committee of Anhui Medical University, and all patients and controls gave their written informed consent. The procedures followed the tenets of the Declaration of Helsinki.

Disclosures

None.

Supplementary material

10067_2017_3887_MOESM1_ESM.docx (17 kb)
ESM 1 (DOCX 16 kb).

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Copyright information

© International League of Associations for Rheumatology (ILAR) 2017

Authors and Affiliations

  • Renfang Han
    • 1
    • 2
  • Xiao Yang
    • 1
    • 2
  • Mengya Chen
    • 1
    • 2
  • Xu Zhang
    • 1
    • 2
  • Yaping Yuan
    • 1
    • 2
  • Xingxing Hu
    • 1
    • 2
  • Mengmeng Wang
    • 1
    • 2
  • Rui Liu
    • 1
    • 2
  • Yubo Ma
    • 1
    • 2
  • Jiajia Yang
    • 1
    • 2
  • Shengqian Xu
    • 3
  • Zongwen Shuai
    • 3
  • Shanqun Jiang
    • 4
  • Faming Pan
    • 1
    • 2
  1. 1.Department of Epidemiology and Biostatistics, School of Public HealthAnhui Medical UniversityHefeiChina
  2. 2.The Key Laboratory of Major Autoimmune DiseasesAnhui Medical UniversityHefeiChina
  3. 3.Department of Rheumatism and ImmunityThe First Affiliated Hospital of Anhui Medical UniversityHefeiChina
  4. 4.School of Life SciencesAnhui UniversityHefeiChina

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