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STAT4 rs7574865 G/T polymorphism is associated with rheumatoid arthritis and disease activity, but not with anti-CCP antibody levels in a Mexican population

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Abstract

Rheumatoid arthritis (RA) is a systemic autoimmune disease in whose etiology genetic factors are known to play an important role. Among the genes associated with RA, STAT4 could be an important factor in conducting helper T cells toward the pro-inflammatory Th1 and Th17 lineages. The aim of this study is to determine the association of the STAT4 polymorphism rs7574865 with RA, disease activity, and anti-cyclic citrullinated peptide (CCP) antibody levels in a Mexican population. Genotyping was carried out using the Taqman® system from Applied Biosystems in 140 patients with RA and 150 healthy subjects. Disease activity was evaluated by a rheumatologist using the DAS28 and Spanish-HAQ-DI instruments. Anti-CCP levels were determined by ELISA. Associations of the genotypes of rs7574865 with DAS28, HAQ, and anti-CCP antibody levels with RA were determined. Findings showed that the GT and TT genotypes and the T allele from rs7574865 were all associated as risk factors for RA, independently of their anti-CCP status. An association with moderate-to-high disease activity (DAS28 ≥ 3.2) was also found. Additionally, patients with the GT or TT genotypes showed lower HAQ values than those who carried the GG genotype. No differences in anti-CCP antibody levels or DAS28 and genotypes were found. This work supports the association of the STAT4 rs7574865 polymorphism with RA and disease activity, but not with anti-CCP antibody levels in a Mexican population.

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Acknowledgments

This work was supported by the Integral Program for Institutional Improvement (PIFI-2011-18MSU0019M-05) and the Improvement of Quality of Educational Institutions Program (PROFOCIE-2014-18MSU0019M-04-01). Raquel Rocío Hernández Pacheco was funded as a Fellow of Mexico’s National Science and Technology Council (CONACYT), while América del Carmen Castro Zambrano was a Fellow of the National Organization for Scholarships for Higher Education of Mexico’s Department of Public Education (SEP).

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Authors and Affiliations

  1. Unidad Académica de Ciencias Químico Biológicas y Farmacéuticas-Universidad Autónoma de Nayarit, Cd. de la Cultura, Amado Nervo s/n, CP 63190, Tepic, Nayarit, Mexico

    Ma. de Jesús Durán-Avelar, Norberto Vibanco-Pérez, América del Carmen Castro-Zambrano & José Francisco Zambrano-Zaragoza

  2. Maestría en Salud Pública, Unidad Académica de Medicina, Universidad Autónoma de Nayarit, Tepic, Nayarit, Mexico

    Raquel Rocío Hernández-Pacheco

  3. Clínica de Reumatología, Servicio de Medicina Interna, Instituto Mexicano del Seguro Social HGZ No. 1, Tepic, Nayarit, Mexico

    Liliana Ortiz-Martínez

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  1. Ma. de Jesús Durán-Avelar
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  2. Norberto Vibanco-Pérez
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  6. José Francisco Zambrano-Zaragoza
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Correspondence to José Francisco Zambrano-Zaragoza.

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Durán-Avelar, M.d.J., Vibanco-Pérez, N., Hernández-Pacheco, R.R. et al. STAT4 rs7574865 G/T polymorphism is associated with rheumatoid arthritis and disease activity, but not with anti-CCP antibody levels in a Mexican population. Clin Rheumatol 35, 2909–2914 (2016). https://doi.org/10.1007/s10067-016-3320-z

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  • DOI: https://doi.org/10.1007/s10067-016-3320-z

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