Clinical Rheumatology

, Volume 28, Issue 12, pp 1431–1435 | Cite as

Correlation of synovial fluid leptin concentrations with the severity of osteoarthritis

  • Jung Hoei Ku
  • Choon Key Lee
  • Bo Sun Joo
  • Byeong Min An
  • Seung Hyun Choi
  • Tae Hyun Wang
  • Hyung Lae Cho
Brief Report

Abstract

Leptin is known to play an important role in the pathophysiology of osteoarthritis (OA). This study investigated whether synovial fluid (SF) leptin level is related to the radiographic severity of OA and its role as a quantitative marker for the detection of OA. SF was obtained from 42 OA patients who underwent knee surgery and 10 who had no abnormality of articular cartilage during arthroscopic examination. The progression of OA was classified by Kellgren–Lawrence grading scale. The concentrations of leptin were measured with commercial enzyme-linked-immunosorbent serologic assay kits. Median leptin concentrations in SF were significantly higher in OA patients (median 4.40 ng/ml; range 0.5–15.8) compared to controls (median 2.05 ng/ml; range 1.0–4.6; P = 0.006). SF leptin levels showed significant difference according to the severity of OA (P = 0.0125). Median SF leptin level was highest in stage IV patients (11.1 ng/ml), which was significantly higher compared to all other groups including controls (P < 0.05). Age showed a significant positive correlation with leptin concentrations in OA patients (P < 0.05), but not in controls. These results demonstrate that SF leptin concentrations were closely related to the radiographic severity of OA, suggesting that SF leptin levels could be used as an effective marker for quantitative detection of OA.

Keywords

Biochemical markers Leptin Osteoarthritis Synovial fluid 

References

  1. 1.
    Garnero P, Landewe R, Boers M et al (2002) Association of baseline levels of markers of bone and cartilage degradation with long-term progression of joint damage in patients with early rheumatoid arthritis: the COBRA study. Arthritis Rheum 11:2847–2856CrossRefGoogle Scholar
  2. 2.
    Kellgren JH, Lawrence JS (1957) Radiological assessment of osteo-arthrosis. Ann Rheum Dis 16:494–502CrossRefPubMedGoogle Scholar
  3. 3.
    Dahlberg L, Ryd L, Heinegard D, Lohmander LS (2003) Proteoglycan fragments in joint fluid. Influence of arthrosis and inflammation. Acta Orthop Scand 4:417–423Google Scholar
  4. 4.
    Maiotti M, Monteleone G, Tarantino U, Fasciglione GF, Marini S, Coletta M (2000) Correlation between osteoarthritic cartilage damage and levels of proteinases and proteinase inhibitors in synovial fluid from the knee joint. Arthroscopy 5:522–526CrossRefGoogle Scholar
  5. 5.
    Takahashi M, Naito K, Abe M, Sawada T, Nagano A (2004) Relationship between radiographic grading of osteoarthritis and the biochemical markers for arthritis in knee osteoarthritis. Arthritis Res Ther 3:R208–R212CrossRefGoogle Scholar
  6. 6.
    Chambers MG, Bayliss MT, Mason RM (1997) Chondrocyte cytokine and growth factor expression in murine osteoarthritis. Osteoarthritis Cartilage 5:301–308CrossRefPubMedGoogle Scholar
  7. 7.
    Goldenberg MM (1999) Etanercept, a novel drug for the treatment of patients with severe, active rheumatoid arthritis. Clin Ther 1:75–87CrossRefGoogle Scholar
  8. 8.
    Webb GR, Westacott CI, Elson CJ (1997) Chondrocyte tumor necrosis factor receptors and focal loss of cartilage in osteoarthritis. Osteoarthritis Cartilage 6:427–437CrossRefGoogle Scholar
  9. 9.
    Westacott CI, Sharif M (1996) Cytokines in osteoarthritis: mediators or markers of joint destruction? Semin Arthritis Rheum 4:254–272CrossRefGoogle Scholar
  10. 10.
    Chehab FF, Mounzih K, Lu R, Lim ME (1997) Early onset of reproductive function in normal female mice treated with leptin. Science 5296:88–90CrossRefGoogle Scholar
  11. 11.
    Sierra-Honigmann MR, Nath AK, Murakami C et al (1998) Biological action of leptin as an angiogenic factor. Science 5383:1683–1686CrossRefGoogle Scholar
  12. 12.
    Figenschau Y, Knutsen G, Shahazeydi S, Johansen O, Sveinbjornsson B (2001) Human articular chondrocytes express functional leptin receptors. Biochem Biophys Res Commun 287:190–197CrossRefPubMedGoogle Scholar
  13. 13.
    Dumond H, Presle N, Terlain B et al (2003) Evidence for a key role of leptin in osteoarthritis. Arthritis Rheum 11:3118–3129CrossRefGoogle Scholar
  14. 14.
    Van Beuningen HM, Glansbeek HL, van der Kraan PM, van den Berg WB (2000) Osteoarthritis-like changes in the murine knee joint resulting from intra-articular transforming growth factor-beta injections. Osteoarthritis Cartilage 1:25–33CrossRefGoogle Scholar
  15. 15.
    Redini F, Mauviel A, Pronost S, Loyau G, Pujol JP (1993) Transforming growth factor beta exerts opposite effects from interleukin-1 beta on cultured rabbit articular chondrocytes through reduction of interleukin-1 receptor expression. Arthritis Rheum 1:44–50CrossRefGoogle Scholar
  16. 16.
    Teichtahl AJ, Wluka AE, Proietto J, Cicuttini FM (2005) Obesity and the female sex, risk factors for knee osteoarthritis that may be attributable to systemic or local leptin biosynthesis and its cellular effects. Med Hypotheses 65:312–315CrossRefPubMedGoogle Scholar
  17. 17.
    Simopoulou T, Malizos KN, Iliopoulos D et al (2007) Differential expression of leptin and leptin’s receptor isoform (Ob-Rb) mRNA between advanced and minimally affected osteoarthritic cartilage; effect on cartilage metabolism. Osteoarthritis Cartilage 8:872–883CrossRefGoogle Scholar
  18. 18.
    Fernandes JC, Martel-Pelletier J, Pelletier JP (2002) The role of cytokines in osteoarthritis pathophysiology. Biorheology 39:237–246PubMedGoogle Scholar
  19. 19.
    Tetlow LC, Adlam DJ, Woolley DE (2001) Matrix metalloproteinase and proinflammatory cytokine production by chondrocytes of human osteoarthritic cartilage: associations with degenerative changes. Arthritis Rheum 3:585–594CrossRefGoogle Scholar
  20. 20.
    Otero M, Gomez Reino JJ, Gualillo O (2003) Synergistic induction of nitric oxide synthase type II: in vitro effect of leptin and interferon-gamma in human chondrocytes and ATDC5 chondrogenic cells. Arthritis Rheum 2:404–409CrossRefGoogle Scholar
  21. 21.
    Otero M, Lago R, Lago F, Reino JJ, Gualillo O (2005) Signalling pathway involved in nitric oxide synthase type II activation in chondrocytes: synergistic effect of leptin with interleukin-1. Arthritis Res Ther 3:R581–R591CrossRefGoogle Scholar
  22. 22.
    Uesaka S, Nakayama Y, Yoshihara K, Ito H (2002) Significance of chondroitin sulfate isomers in the synovial fluid of osteoarthritis patients. J Orthop Sci 2:232–237CrossRefGoogle Scholar
  23. 23.
    Blum WF, Englaro P, Hanitsch S et al (1997) Plasma leptin levels in healthy children and adolescents: dependence on body mass index, body fat mass, gender, pubertal stage, and testosterone. J Clin Endocrinol Metab 9:2904–2910CrossRefGoogle Scholar
  24. 24.
    Hickey MS, Israel RG, Gardiner SN et al (1996) Gender differences in serum leptin levels in humans. Biochem Mol Med 1:1–6CrossRefGoogle Scholar

Copyright information

© Clinical Rheumatology 2009

Authors and Affiliations

  • Jung Hoei Ku
    • 1
  • Choon Key Lee
    • 1
  • Bo Sun Joo
    • 2
  • Byeong Min An
    • 2
  • Seung Hyun Choi
    • 1
  • Tae Hyun Wang
    • 1
  • Hyung Lae Cho
    • 1
  1. 1.Department of Orthopedic SurgeryGood Samsun HospitalBusanSouth Korea
  2. 2.Good Life Science InstituteGood Moonhwa HospitalBusanSouth Korea

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