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Oxidant/antioxidant status in men with Behçet’s disease

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Abstract

Behçet’s disease (BD) is a chronic, progressive disorder that affects many systems of the body including the eye. The aim of this study was to assess whether the increase in oxidative stress in the affected tissues is reflected by lipid peroxidation and to check for alterations in antioxidants and antioxidant enzyme activities in patients with BD. Erythrocyte antioxidant potential (AOP), glutathione (GSH) and GSH-dependent enzymes (glutathione peroxidase (GSH-Px), glutathione reductase (GRD) and glutathione-S-transferase (GST), catalase (CAT), Cu–Zn superoxide dismutase (Cu–Zn SOD) activities, malondialdehyde (MDA) and some trace elements (zinc, Zn; copper, Cu; manganese, Mn) levels in men with BD. Erythrocyte CAT, GSH-Px activities, MDA, GSH, AOP and serum Zn values were significantly lower in patients with BD than in the control group. However, erythrocyte Cu–Zn SOD, GRD activities, erythrocyte sedimentation rate (ESR), serum C-reactive protein (CRP) and Cu values were significantly higher in patients with BD than in the control group, but GST activity and serum Mn values were unchanged. In conclusion, our results confirm the presence of oxidative stress in patients with BD and suggest that the severity of BD may arise from impaired antioxidant mechanisms. Therapy with antioxidants may lead to the increase in the antioxidant defense system and thus improvement in clinical symptoms.

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Abbreviations

AOP:

antioxidant potential

BD:

Behçet’s disease

CAT:

catalase

CRP:

C-reactive protein

Cu–Zn SOD:

Cu–Zn superoxide dismutase

ESR:

erythrocyte sedimentation rate

DTNB:

5, 5′-dithiobis (2-nitrobenzoic acid)

GSH:

reduced glutathione

GSH-Px:

glutathione peroxidase

GRD:

glutathione reductase

H2O2 :

hydrogen peroxide

MDA:

malondialdehyde

NADPH:

reduced nicotinamide adenine dinucleotide phosphate

NADP+ :

oxidized nicotinamide adenine dinucleotide phosphate

NBT:

nitroblue tetrazolium

NO:

nitric oxide

\( {\text{O}}^{{\raise0.145em\hbox{${\scriptscriptstyle \bullet}$} - }}_{2} \) :

superoxide anion radical

\( ^{\raise0.145em\hbox{${\scriptscriptstyle \bullet}$}} {\text{OH}}^{ - } \) :

hydroxyl radicals

ONOO− :

peroxynitrite

RNS:

reactive nitrogen species

ROS:

reactive oxygen species

TBA:

thiobarbituric acid

TBARS:

total thiobarbituric acid-reactive substances

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Authors and Affiliations

  1. Department of Biochemistry, Nenehatun Obstetric and Gynecology Hospital, Erzurum, Turkey

    Seyithan Taysi

  2. Department of Biochemistry, Medical School, Atatürk University, Erzurum, Turkey

    Berna Demircan

  3. Department of Dermatology, 100 Yıl University, Van, Turkey

    Necmettın Akdeniz

  4. Department of Dermatology, Medical School, Atatürk University, Erzurum, Turkey

    Mustafa Atasoy

  5. Department of Immunology, Medical School, Atatürk University, Erzurum, Turkey

    Refik Ali Sari

  6. Nenehatun Kadın Doğum Hastanesi Çat yolu, Biyokimya Laboratuarı, Erzurum, Turkey

    Seyithan Taysi

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  1. Seyithan Taysi
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  2. Berna Demircan
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  3. Necmettın Akdeniz
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  4. Mustafa Atasoy
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Correspondence to Seyithan Taysi.

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Taysi, S., Demircan, B., Akdeniz, N. et al. Oxidant/antioxidant status in men with Behçet’s disease. Clin Rheumatol 26, 418–422 (2007). https://doi.org/10.1007/s10067-006-0513-x

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  • DOI: https://doi.org/10.1007/s10067-006-0513-x

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