Skip to main content

Advertisement

Log in

Clinical significance of IL-18, IL-15, IL-12 and TNF-α measurement in rheumatoid arthritis

  • Original Article
  • Published:
Clinical Rheumatology Aims and scope Submit manuscript

Abstract

The aim of the study was to evaluate the clinical significance of serum (S) and synovial fluid (SF) interleukin (IL)-18, IL-15, IL-12 and the tumor necrosis factor alpha (TNF-α) measurements in relation to laboratory and clinical measures of disease activity of patients with active rheumatoid arthritis (RA). Sixty-four patients with RA and 25 patients with osteoarthritis (OA) were included in this study. RA activity was determined using the Disease Activity Score (DAS) 28 index. Concentrations of IL-18, IL-15, IL-12 and TNF-α were measured by ELISA. Serum C-reactive protein (CRP) levels were also determined. Cross-sectional correlations between S and SF levels of cytokines and values of DAS 28 index were calculated. The results have shown that IL-18, IL-15, IL-12 and TNF-α levels in S and SF of patients with RA were significantly higher than the levels obtain from patients with OA (p<0.01). Significantly higher levels of IL-18, IL-15 and TNF-α were found in the SF compared to the S of patients with RA (p<0.01). Significantly higher S and SF levels of all four cytokines and serum CRP values were found in RA patients with high disease activity (DAS 28>5.1) compared to those with mild (DAS 28>3.2) and low disease activity (DAS 28>2.6) (p<0.01). Serum and SF concentrations of all four cytokines positively correlated with DAS 28 index values, i.e., disease activity. A poor correlation was found for S and SF IL-12 whereas the highest coefficient of correlation was found for SF IL-18 (r=0.879, p<0.01), and SF TNF-α (r=0.827, p<0.01) and disease activity in this study. Strong correlation was found between SF TNF-α and SF IL-18 levels (r=0.732, p<0.01). In conclusion, SF IL-18 and TNF-α levels in RA patients are good indicators of disease activity. The results obtained support the use of the DAS in clinical practice as a reliable method in assessing disease activity in RA patients.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Kawashima M, Miossec P (2004) Decreased response to IL-12 and IL-18 of peripheral blood cells in rheumatoid arthritis. Arthritis Res Ther 6:R39–R45

    Article  PubMed  CAS  Google Scholar 

  2. Joosten LA, Radstake TR, Lubberts E, van den Bersselaar LA, van Riel PL, van Lent PL et al (2003) Association of interleukin-18 expression with enhanced levels of both interleukin-1beta and tumor necrosis alpha in knee synovial tissue of patients with rheumatoid arthritis. Arthritis Rheum 48(2):339–347

    Article  PubMed  CAS  Google Scholar 

  3. Leung PB, McInnes BI, Esfandiari E, Wei XQ, Liew YF (2000) Combined effects of IL-12 and IL-18 on the induction of collagen-induced arthritis. J Immunol 164:6495–6502

    PubMed  CAS  Google Scholar 

  4. Gracie AJ, Forsey JR, Ling Chan W, Gilmour A, Leung PB, Greer RM et al (1999) A proinflammatory role for IL-18 in rheumatoid arthritis. J Clin Invest 104:1393–1401

    Article  PubMed  CAS  Google Scholar 

  5. McInnes BI, Gracie AJ, Hornett M, Hornett W, Liew YF (2003) New strategies to control inflammatory synovitis: interleukin 15 and beyond. Ann Rheum Dis 62:ii51–ii54

    Article  PubMed  CAS  Google Scholar 

  6. Prevoo LM, Hof AM, Kuper HH, Leeuwen AM, Putte BL, Riel LP (1995) Modified disease activity scores that include twenty-eight-joint counts. Arthritis Rheum 38:44–48

    Article  PubMed  CAS  Google Scholar 

  7. Stone AM, Payne U, Pacheco-Tena C, Inman DR (2004) Cytokine correlates of clinical response patterns to infliximab treatment of ankylosing spondylitis. Ann Rheum Dis 63:84–87

    Article  PubMed  CAS  Google Scholar 

  8. Liew YF, McInnes BI (2002) Role of interleukin 15 and interleukin 18 in inflammatory response. Ann Rheum Dis 61:ii100–ii102

    PubMed  CAS  Google Scholar 

  9. Bresnihan B, Roux-Lombard P, Murphy E, Kane D, FitzGerald O, Dayer MJ (2002) Serum interleukin 18 and interleukin 18 binding protein in rheumatoid arthritis. Ann Rheum Dis 61:726–729

    Article  PubMed  CAS  Google Scholar 

  10. Klimiuk AP, Sierakowski S, Latosiewicz R, Cylwik B, Skowronski J, Chwiecko J (2001) Serum cytokines in different histological variants of rheumatoid arthritis. J Rheumatol 28:1211–1217

    PubMed  CAS  Google Scholar 

  11. Gonzalez-Alvaro I, Ortiz AM, Garcia-Vicuna R, Balsa A, Pascual-Salcedo Laffon A (2003) Increased serum levels of interleukin-15 in rheumatoid arthritis with long-term disease. Clin Exp Rheumatol 21(5):639–642

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Nada Pejnovic.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Petrovic-Rackov, L., Pejnovic, N. Clinical significance of IL-18, IL-15, IL-12 and TNF-α measurement in rheumatoid arthritis. Clin Rheumatol 25, 448–452 (2006). https://doi.org/10.1007/s10067-005-0106-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10067-005-0106-0

Keywords

Navigation