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Molecules and Cells

, Volume 35, Issue 6, pp 481–488 | Cite as

Requirement of vesicle-associated membrane protein 721 and 722 for sustained growth during immune responses in Arabidopsis

  • Hye Sup Yun
  • Mark Kwaaitaal
  • Naohiro Kato
  • Changhyun Yi
  • Sohyeon Park
  • Masa H. Sato
  • Paul Schulze-LefertEmail author
  • Chian KwonEmail author
Research Article

Abstract

Extracellular immune responses to ascomycete and oomycete pathogens in Arabidopsis are dependent on vesicle-associated secretion mediated by the SNARE proteins PEN1 syntaxin, SNAP33 and endomembrane-resident VAMP721/722. Continuous movement of functional GFP-VAMP722 to and from the plasma membrane in non-stimulated cells reflects the second proposed function of VAMP721/722 in constitutive secretion during plant growth and development. Application of the bacterium-derived elicitor flg22 stabilizes VAMP721/722 that are otherwise constitutively degraded via the 26S proteasome pathway. Depletion of VAMP721/722 levels by reducing VAMP721/722 gene dosage enhances flg22-induced seedling growth inhibition in spite of elevated VAMP721/722 abundance. We therefore propose that plants prioritize the deployment of the corresponding secretory pathway for defense over plant growth. Interstingly, VAMP721/722 specifically interact in vitro and in vivo with the plasma membrane syntaxin SYP132 that is required for plant growth and resistance to bacteria. This suggests that the plant growth/immunity-involved VAMP721/722 form SNARE complexes with multiple plasma membrane syntaxins to discharge cue-dependent cargo molecules.

Keywords

plant growth plant immune responses PM syntaxins secretion VAMP721/722 

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Copyright information

© The Korean Society for Molecular and Cellular Biology and Springer Netherlands 2013

Authors and Affiliations

  • Hye Sup Yun
    • 1
    • 2
  • Mark Kwaaitaal
    • 1
  • Naohiro Kato
    • 3
  • Changhyun Yi
    • 4
  • Sohyeon Park
    • 4
  • Masa H. Sato
    • 5
  • Paul Schulze-Lefert
    • 1
    Email author
  • Chian Kwon
    • 1
    • 4
    • 6
    Email author
  1. 1.Deparment of Plant Microbe InteractionsMax-Planck-Institut für ZüchtungsforschungKölnGermany
  2. 2.Biological Sciences Major, College of Biological Science and BiotechnologyKonkuk UniversitySeoulKorea
  3. 3.Department of Biological SciencesLouisiana State UniversityNew OrleansUSA
  4. 4.Department of Molecular BiologyDankook UniversityYonginKorea
  5. 5.Laboratory of Cellular Dynamics, Graduate School of Life and Environmental SciencesKyoto Prefectural UniversityKyotoJapan
  6. 6.Department of Integrated Molecular SciencesDankook UniversityYonginKorea

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