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Extracellular HIV-1 Tat induces human beta-defensin-2 production via NF-kappaB/AP-1 dependent pathways in human B cells

  • Published:
Molecules and Cells

Abstract

Defensins, a family of antimicrobial peptides, are one of the first lines of host defense. Human beta-defensins (hBD) such as hBD-2 and -3 have anti-HIV activity. Previous studies have shown that HIV-1 virion can induce the expression of hBD, although the exact components of HIV-1 virion that are responsible for hBD expression have not yet been elucidated. In this study, we examined the effect of HIV-1 Tat on the expression of hBD in B cells. Stimulation of B cells with HIV-1 Tat protein significantly increased the mRNA and protein levels of hBD-2. HIV-1 Tat also induced the activation of a reporter gene for hBD-2 in a dose-dependent manner in B cells. Pretreatment of B cells with a JNK inhibitor suppressed HIV-1 Tat-induced hBD-2 expression. Pretreatment of B cells with AP-1 inhibitors or NF-κB inhibitors led to a decrease in HIV-1 Tat-induced protein and mRNA expression of hBD-2. Taken together, our results indicate that HIV-1 Tat can up-regulate the expression of hBD-2 via JNK-NF-κB/AP-1-dependent pathways in human B cells.

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Authors and Affiliations

  1. Department of Microbiology, College of Medicine, Hallym University, Chunchon, 200-702, Korea

    Hyung-Joo Kwon

  2. Department of Biological Science, College of Natural Sciences, Sungkyunkwan University, Suwon, 440-746, Korea

    Yong Soo Bae

  3. Department of Biomedical Science, Research Institute for Bioscience and Biotechnology, Hallym University, Chunchon, 200-702, Korea

    Sung Mi Ju, Ah Ra Goh, Dong-Joo Kwon, Gi Soo Youn, Soo Young Choi & Jinseu Park

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  1. Sung Mi Ju
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  2. Ah Ra Goh
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  3. Dong-Joo Kwon
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  4. Gi Soo Youn
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  5. Hyung-Joo Kwon
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  7. Soo Young Choi
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  8. Jinseu Park
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Correspondence to Jinseu Park.

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Ju, S.M., Goh, A.R., Kwon, DJ. et al. Extracellular HIV-1 Tat induces human beta-defensin-2 production via NF-kappaB/AP-1 dependent pathways in human B cells. Mol Cells 33, 335–341 (2012). https://doi.org/10.1007/s10059-012-2287-0

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  • DOI: https://doi.org/10.1007/s10059-012-2287-0

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