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Crystal structure of malonyl CoA-Acyl carrier protein transacylase from Xanthomanous oryzae pv. oryzae and its proposed binding with ACP

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Molecules and Cells

Abstract

Xanthomonas oryzae pv. oryzae (Xoo) is a plant bacterial pathogen that causes bacterial blight (BB) disease, resulting in serious production losses of rice. The crystal structure of malonyl CoA-acyl carrier protein transacylase (XoMCAT), encoded by the gene fabD (Xoo0880) from Xoo, was determined at 2.3 Å resolution in complex with N-cyclohexyl-2-aminoethansulfonic acid. Malonyl CoA-acyl carrier protein transacylase transfers malonyl group from malonyl CoA to acyl carrier protein (ACP). The transacylation step is essential in fatty acid synthesis. Based on the rationale, XoMCAT has been considered as a target for antibacterial agents against BB. Protein-protein interaction between XoMCAT and ACP was also extensively investigated using computational docking, and the proposed model revealed that ACP bound to the cleft between two XoMCAT subdomains.

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Authors and Affiliations

  1. Department of Advanced Technology Fusion, Konkuk University, Seoul, 143-701, Korea

    Sampath Natarajan, Jin-Kwang Kim, Tae-Kyun Jung, Thanh Thi Ngoc Doan, Ho-Phuong-Thuy Ngo, Myoung-Ki Hong, Seunghwan Kim, Viet Pham Tan, Seok Joon Ahn, Yesun Han & Lin-Woo Kang

  2. Department of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea

    Seok Joon Ahn

  3. Department of Biological Sciences, Myongji University, Yongin, 449-728, Korea

    Sang Hee Lee

  4. Department of Green Life Science, College of Convergence, Sangmyung University, Seoul, 110-743, Korea

    Yeh-Jin Ahn

  5. Center for Biotechnology Research in UBITA (CBRU), Seoul, 143-701, Korea

    Lin-Woo Kang

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  1. Sampath Natarajan
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  2. Jin-Kwang Kim
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  3. Tae-Kyun Jung
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  9. Seok Joon Ahn
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  10. Sang Hee Lee
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  12. Yeh-Jin Ahn
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  13. Lin-Woo Kang
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Corresponding authors

Correspondence to Yeh-Jin Ahn or Lin-Woo Kang.

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These authors contributed equally to this work.

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Natarajan, S., Kim, JK., Jung, TK. et al. Crystal structure of malonyl CoA-Acyl carrier protein transacylase from Xanthomanous oryzae pv. oryzae and its proposed binding with ACP. Mol Cells 33, 19–25 (2012). https://doi.org/10.1007/s10059-012-2155-y

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  • DOI: https://doi.org/10.1007/s10059-012-2155-y

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