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Ectopic expression of Capsicum-specific cell wall protein Capsicum annuum senescence-delaying 1 (CaSD1) delays senescence and induces trichome formation in Nicotiana benthamiana

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Molecules and Cells

Abstract

Secreted proteins are known to have multiple roles in plant development, metabolism, and stress response. In a previous study to understand the roles of secreted proteins, Capsicum annuum secreted proteins (CaS) were isolated by yeast secretion trap. Among the secreted proteins, we further characterized Capsicum annuum senescence-delaying 1 (CaSD1), a gene encoding a novel secreted protein that is present only in the genus Capsicum. The deduced CaSD1 contains multiple repeats of the amino acid sequence KPPIHNHKPTDYDRS. Interestingly, the number of repeats varied among cultivars and species in the Capsicum genus. CaSD1 is constitutively expressed in roots, and Agrobacterium-mediated transient overexpression of CaSD1 in Nicotiana benthamiana leaves resulted in delayed senescence with a dramatically increased number of trichomes and enlarged epidermal cells. Furthermore, senescence- and cell division-related genes were differentially regulated by CaSD1-overexpressing plants. These observations imply that the pepper-specific cell wall protein CaSD1 plays roles in plant growth and development by regulating cell division and differentiation.

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Authors and Affiliations

  1. Department of Plant Science, Plant Genomics and Breeding Institute, Seoul National University, Seoul, 151-921, Korea

    Eunyoung Seo, Seon-In Yeom, Heejin Jeong, Byoung-Cheorl Kang & Doil Choi

  2. Seeders Inc., Daejeon, 305-509, Korea

    SungHwan Jo

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  1. Eunyoung Seo
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  2. Seon-In Yeom
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  3. SungHwan Jo
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  5. Byoung-Cheorl Kang
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  6. Doil Choi
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Correspondence to Doil Choi.

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Seo, E., Yeom, SI., Jo, S. et al. Ectopic expression of Capsicum-specific cell wall protein Capsicum annuum senescence-delaying 1 (CaSD1) delays senescence and induces trichome formation in Nicotiana benthamiana . Mol Cells 33, 415–422 (2012). https://doi.org/10.1007/s10059-012-0017-2

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  • DOI: https://doi.org/10.1007/s10059-012-0017-2

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