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Molecules and Cells

, Volume 31, Issue 2, pp 165–173 | Cite as

Immunostimulating activity by polysaccharides isolated from fruiting body of Inonotus obliquus

  • Dong Pil Won
  • Jong Seok Lee
  • Duck Soo Kwon
  • Keun Eok Lee
  • Won Cheol Shin
  • Eock Kee HongEmail author
Article

Abstract

In this study, we investigated the immunostimulating activity of polysaccharides isolated from fruiting body of Inonotus obliquus (PFIO). Additionally, the signaling pathway of PFIO-mediated macrophage activation was investigated in RAW264.7 macrophage cells. We found that PFIO was capable of promoting NO/ROS production, TNF-α secretion and phagocytic uptake in macrophages, as well as cell proliferation, comitogenic effect and IFN-γ/IL-4 secretion in mouse splenocytes. PFIO was able to induce the phosphorylation of three MAPKs as well as the nuclear translocation of NF-κB, resulting in activation of RAW264.7 macrophages. PFIO also induced the inhibition of TNF-α secretion by anti-TLR2 mAb, consequently, PFIO might be involved in TNF-α secretion via the TLR2 receptor. In addition, our results showed that oral administration of PFIO suppressed in vivo growth of melanoma tumor in tumorbearing mice. In conclusion, our experiments presented that PFIO effectively promotes macrophage activation through the MAPK and NF-κB signaling pathways, suggesting that PFIO may potentially regulate the immune response.

Keywords

immunostimulating activity Inonotus obliquus MAPKs NF-κpattern-recognition receptor 

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Copyright information

© The Korean Society for Molecular and Cellular Biology and Springer Netherlands 2011

Authors and Affiliations

  • Dong Pil Won
    • 1
  • Jong Seok Lee
    • 1
  • Duck Soo Kwon
    • 1
  • Keun Eok Lee
    • 1
  • Won Cheol Shin
    • 1
  • Eock Kee Hong
    • 1
    Email author
  1. 1.Department of Bioengineering and TechnologyKangwon National UniversityChuncheonKorea

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