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Basic fibroblast growth factor increases intracellular magnesium concentration through the specific signaling pathways

  • Published:
Molecules and Cells

Abstract

Basic fibroblast growth factor (bFGF) plays an important role in angiogenesis. However, the underlying mechanisms are not clear. Mg2+ is the most abundant intracellular divalent cation in the body and plays critical roles in many cell functions. We investigated the effect of bFGF on the intracellular Mg2+ concentration ([Mg2+]i) in human umbilical vein endothelial cells (HUVECs). bFGF increased [Mg2+]i in a dose-dependent manner, independent of extracellular Mg2+. This bFGF-induced [Mg2+]i increase was blocked by tyrosine kinase inhibitors (tyrphostin A-23 and genistein), phosphatidylinositol 3-kinase (PI3K) inhibitors (wortmannin and LY294002) and a phospholipase Cγ (PLCγ) inhibitor (U73122). In contrast, mitogen-activated protein kinase inhibitors (SB202190 and PD98059) did not affect the bFGF-induced [Mg2+]i increase. These results suggest that bFGF increases the [Mg2+]i from the intracellular Mg2+ stores through the tyrosine kinase/PI3K/PLCγ-dependent signaling pathways.

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Author notes

  1. Bing-Zhe Hong

    Present address: Department of Cardiology, Xinhua Hospital, Dalian University Medical School, Dalian, 116021, China

Authors and Affiliations

  1. Department of Pharmacology, Chonbuk National University Medical School, Jeonju, 560-182, Korea

    Bing-Zhe Hong, Sun-Ah Park, Han-Na Kim, Tian-Ze Ma, Han-Gyu Kim & Yong-Geun Kwak

  2. Department of Pharmacology, Chonbuk National University College of Veterinary Medicine, Jeonju, 561-180, Korea

    Hyung-Sub Kang

  3. Division of Biological Sciences, Chonbuk National University, Jeonju, 561-756, Korea

    Hwan-Gyu Kim

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  1. Bing-Zhe Hong
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Correspondence to Yong-Geun Kwak.

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Hong, BZ., Park, SA., Kim, HN. et al. Basic fibroblast growth factor increases intracellular magnesium concentration through the specific signaling pathways. Mol Cells 28, 13–17 (2009). https://doi.org/10.1007/s10059-009-0103-2

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  • DOI: https://doi.org/10.1007/s10059-009-0103-2

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