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Age-dependent pathogenesis of murine gammaherpesvirus 68 infection of the central nervous system

  • Published:
Molecules and Cells

Abstract

Gammaherpesvirus infection of the central nervous system (CNS) has been linked to various neurological diseases, including meningitis, encephalitis, and multiple sclerosis. However, little is known about the interactions between the virus and the CNS in vitro or in vivo. Murine gammaherpesvirus 68 (MHV-68 or γHV-68) is genetically related and biologically similar to human gammaherpesviruses, thereby providing a tractable animal model system in which to study both viral pathogenesis and replication. In the present study, we show the successful infection of cultured neuronal cells, microglia, and astrocytes with MHV-68 to various extents. Upon intracerebroventricular injection of a recombinant virus (MHV-68/LacZ) into 4–5-week-old and 9–10-week-old mice, the 4–5-week-old mice displayed high mortality within 5–7 days, while the majority of the 9–10-week-old mice survived until the end of the experimental period. Until a peak at 3–4 days post-infection, viral DNA replication and gene expression were similar in the brains of both mouse groups, but only the 9–10-week-old mice were able to subdue viral DNA replication and gene expression after 5 days post-infection. Pro-inflammatory cytokine mRNAs of tumor necrosis factor-α, interleukin 1β, and interleukin 6 were highly induced in the brains of the 4–5-week-old mice, suggesting their possible contributions as neurotoxic factors in the agedependent control of MHV-68 replication of the CNS.

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Authors and Affiliations

  1. Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 136-713, Korea

    Hye-Jeong Cho, Sungbum Kim & Moon Jung Song

  2. Department of Microbiology, College of Medicine, Hallym University, Chuncheon, 200-702, Korea

    Sungbum Kim, Hee-Sung Kim, Hyung-Joo Kwon, Yoon-Won Kim & Yong-Sun Kim

  3. Department of Anatomy and Neurobiology, College of Medicine, Hallym University, Chuncheon, 200-702, Korea

    Sung-Eun Kwak & Tae-Cheon Kang

  4. Ilsong Institute of Life Science, Hallym University, Anyang, 431-060, Korea

    Eun-Kyung Choi

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  1. Hye-Jeong Cho
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  2. Sungbum Kim
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  3. Sung-Eun Kwak
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  5. Hee-Sung Kim
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  7. Yoon-Won Kim
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  10. Moon Jung Song
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Correspondence to Moon Jung Song.

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These authors contributed equally to this work.

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Cho, HJ., Kim, S., Kwak, SE. et al. Age-dependent pathogenesis of murine gammaherpesvirus 68 infection of the central nervous system. Mol Cells 27, 105–111 (2009). https://doi.org/10.1007/s10059-009-0011-5

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  • DOI: https://doi.org/10.1007/s10059-009-0011-5

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