Journal of Artificial Organs

, Volume 21, Issue 3, pp 387–391 | Cite as

Wall thickness control in biotubes prepared using type-C mold

  • Takeshi Terazawa
  • Takanori Nishimura
  • Tomohiro Mitani
  • Osamu Ichii
  • Teppei Ikeda
  • Keigo Kosenda
  • Eisuke Tatsumi
  • Yasuhide Nakayama
Brief Communication Tissue Engineering / Regenerative Medicine


A type-C mold based on in-body tissue architecture was previously developed for preparing small-diameter biotube vascular grafts with a 2-mm diameter and approximately 1-mm wall thickness. In this study, the type-C mold was modified for preparing large-diameter biotubes with controlled wall thicknesses. Four types of molds were assembled by inserting silicone center rods (outer diameters 11, 13, 15, 17 mm) into stainless steel cages (inner diameter 19 mm) and surgically embedded in the abdominal subcutaneous pouches of Holstein cows. After 8–12 weeks, connective tissues occupied the rod–cage gap in the molds to form biotubes. The wall thickness of the biotubes obtained after removing the molds was approximately 1–3 mm, which corresponded to approximately 80% of each gap distance. The breaking strength almost linearly increased with the wall thickness of the biotubes. The strength of the biotubes with wall thickness over 1.5 mm was higher than that of beagle blood vessels. The thickest biotubes were as strong as bovine pericardium and can be used as an alternative trachea graft because of their adequate lumen-holding force.


In-body tissue architecture Biotube Connective tissue Tubular tissue 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest associated with this work.


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Copyright information

© The Japanese Society for Artificial Organs 2018

Authors and Affiliations

  1. 1.Department of Artificial OrgansNational Cerebral and Cardiovascular Center Research InstituteSuitaJapan
  2. 2.Research Faculty of Agriculture, Graduate School of AgricultureHokkaido UniversitySapporoJapan
  3. 3.Field Science Center for Northern BiosphereHokkaido UniversitySapporoJapan
  4. 4.Laboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary MedicineHokkaido UniversitySapporoJapan
  5. 5.BRAST Livestock ClinicBRAST Inc.SapporoJapan
  6. 6.Shibetsu Veterinary Clinical CenterHokkaidochuo Agricultural Mutual Aid AssociationShibetsuJapan

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