Journal of Artificial Organs

, Volume 18, Issue 3, pp 264–271 | Cite as

Vision maintenance and retinal apoptosis reduction in RCS rats with Okayama University-type retinal prosthesis (OUReP™) implantation

  • Alamusi
  • Toshihiko MatsuoEmail author
  • Osamu Hosoya
  • Kimiko M. Tsutsui
  • Tetsuya Uchida
Original Article Artificial Skin, Muscle, Bone / Joint, Neuron


Photoelectric dye-coupled polyethylene film, designated Okayama University-type retinal prosthesis or OUReP™, generates light-evoked surface electric potentials and stimulates neurons. In this study, the vision was assessed by behavior tests in aged hereditary retinal dystrophic RCS rats with OUReP™, retinal apoptosis and electroretinographic responses were measured in dystrophic eyes with OUReP™. The dye-coupled films, or plain films as a control, were implanted in subretinal space of RCS rats. On behavior tests, RCS rats with dye-coupled films, implanted at the old age of 14 weeks, showed the larger number of head-turning, consistent with clockwise and anticlockwise rotation of a surrounding black-and-white-striped drum, compared with rats with plain films, under the dim (50 lux) and bright (150 lux) conditions in the observation period until the age of 22 weeks (n = 5, P < 0.05, repeated-measure ANOVA). The number of apoptotic cells in retinal sections at the site of dye-coupled film implantation was significantly smaller, compared with the other retinal sites, neighboring the film, or opposite to the film, 5 months after film implantation at the age of 6 weeks (P = 0.0021, Friedman test). The dystrophic eyes of RCS rats with dye-coupled films showed positive responses to maximal light stimulus at a significantly higher rate, compared with the eyes with no treatment (P < 0.05, Chi-square test). Electroretinograms in normal eyes of Wistar rats with dye-coupled or plain films showed significantly decreased amplitudes (n = 14, P < 0.05, repeated-measure ANOVA). In conclusions, vision was maintained in RCS rats with dye-coupled films implanted at the old age. The dystrophic eyes with dye-coupled films showed electroretinographic responses. Five-month film implantation caused no additional retinal changes.


Retinal prosthesis Photoelectric dye RCS rat Apoptosis Electroretinogram 


Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© The Japanese Society for Artificial Organs 2015

Authors and Affiliations

  • Alamusi
    • 1
  • Toshihiko Matsuo
    • 1
    Email author
  • Osamu Hosoya
    • 2
  • Kimiko M. Tsutsui
    • 2
  • Tetsuya Uchida
    • 3
  1. 1.OphthalmologyOkayama University Medical School and Graduate School of Medicine, Dentistry, and Pharmaceutical SciencesOkayamaJapan
  2. 2.NeurogenomicsOkayama University Medical School and Graduate School of Medicine, Dentistry, and Pharmaceutical SciencesOkayamaJapan
  3. 3.Polymer Materials ScienceOkayama University Faculty of Engineering and Graduate School of Natural Science and TechnologyOkayamaJapan

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