Journal of Artificial Organs

, Volume 17, Issue 2, pp 186–192 | Cite as

Platelet and growth factor concentrations in activated platelet-rich plasma: a comparison of seven commercial separation systems

  • Satoshi KushidaEmail author
  • Natsuko KakudoEmail author
  • Naoki Morimoto
  • Tomoya Hara
  • Takeshi Ogawa
  • Toshihito Mitsui
  • Kenji Kusumoto
Original Article Others


Platelet-rich plasma (PRP) is blood plasma that has been enriched with platelets. It holds promise for clinical use in areas such as wound healing and regenerative medicine, including bone regeneration. This study characterized the composition of PRP produced by seven commercially available separation systems (JP200, GLO PRP, Magellan Autologous Platelet Separator System, KYOCERA Medical PRP Kit, SELPHYL, MyCells, and Dr. Shin’s System THROMBO KIT) to evaluate the platelet, white blood cell, red blood cell, and growth factor concentrations, as well as platelet-derived growth factor-AB (PDGF-AB), transforming growth factor beta-1 (TGF-β1), and vascular endothelial growth factor (VEGF) concentrations. PRP prepared using the Magellan Autologous Platelet Separator System and the KYOCERA Medical PRP Kit contained the highest platelet concentrations. The mean PDGF-AB concentration of activated PRP was the highest from JP200, followed by the KYOCERA Medical PRP Kit, Magellan Autologous Platelet Separator System, MyCells, and GLO PRP. TGF-β1 and VEGF concentrations varied greatly among individual samples, and there was almost no significant difference among the different systems, unlike for PDGF. The SELPHYL system produced PRP with low concentrations of both platelets and growth factors. Commercial PRP separation systems vary widely, and familiarity with their individual advantages is important to extend their clinical application to a wide variety of conditions.


Platelet-rich plasma Growth factor Comparison 


Conflict of interest

None of the authors has a financial interest in any of the products, devices, or drugs mentioned in this manuscript.


  1. 1.
    Kakudo N, Minakata T, Mitsui T, Kushida S, Notodihardjo FZ, Kusumoto K. Proliferation-promoting effect of platelet-rich plasma on human adipose-derived stem cells and human dermal fibroblasts. Plast Reconstr Surg. 2008;122:1352–60.PubMedCrossRefGoogle Scholar
  2. 2.
    Kakudo N, Morimoto N, Kushida S, Ogawa T, Kusumoto K. Platelet-rich plasma releasate promotes angiogenesis in vitro and in vivo. Med Mol Morphol (in press). Google Scholar
  3. 3.
    Marx RE. Platelet-rich plasma: evidence to support its use. J Oral Maxillofac Surg. 2004;62:489–96.PubMedCrossRefGoogle Scholar
  4. 4.
    Salcido RS. Autologous platelet-rich plasma in chronic wounds. Adv Skin Wound Care. 2013;26:248.PubMedCrossRefGoogle Scholar
  5. 5.
    Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR. Platelet-rich plasma: growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998;85:638–46.PubMedCrossRefGoogle Scholar
  6. 6.
    de Almeida AM, Demange MK, Sobrado MF, Rodrigues MB, Pedrinelli A, Hernandez AJ. Patellar tendon healing with platelet-rich plasma: a prospective randomized controlled trial. Am J Sports Med. 2012;40:1282–8.PubMedCrossRefGoogle Scholar
  7. 7.
    Man D, Plosker H, Winland-Brown JE. The use of autologous platelet-rich plasma (platelet gel) and autologous platelet-poor plasma (fibrin glue) in cosmetic surgery. Plast Reconstr Surg. 2001;107:229–237, discussion 238–229.Google Scholar
  8. 8.
    Castillo TN, Pouliot MA, Kim HJ, Dragoo JL. Comparison of growth factor and platelet concentration from commercial platelet-rich plasma separation systems. Am J Sports Med. 2011;39:266–71.PubMedCrossRefGoogle Scholar
  9. 9.
    Weibrich G, Kleis WK, Hafner G. Growth factor levels in the platelet-rich plasma produced by 2 different methods: curasan-type PRP kit versus PCCS PRP system. Int J Oral Maxillofac Implant. 2002;17:184–90.Google Scholar
  10. 10.
    Eppley BL, Pietrzak WS, Blanton M. Platelet-rich plasma: a review of biology and applications in plastic surgery. Plast Reconstr Surg. 2006;118:147e–59e.PubMedCrossRefGoogle Scholar
  11. 11.
    Gonshor A. Technique for producing platelet-rich plasma and platelet concentrate: background and process. Int J Periodontics Restor Dent. 2002;22:547–57.Google Scholar
  12. 12.
    Hosgood G. Wound healing: the role of platelet-derived growth factor and transforming growth factor beta. Vet Surg. 1993;22:490–5.PubMedCrossRefGoogle Scholar
  13. 13.
    Civinini R, Nistri L, Martini C, Redl B, Ristori G, Innocenti M. Growth factors in the treatment of early osteoarthritis. Clin Cases Miner Bone Metab. 2013;10:26–9.PubMedCentralPubMedGoogle Scholar
  14. 14.
    Hoeben A, Landuyt B, Highley MS, Wildiers H, Van Oosterom AT, De Bruijn EA. Vascular endothelial growth factor and angiogenesis. Pharmacol Rev. 2004;56:549–80.PubMedCrossRefGoogle Scholar
  15. 15.
    Eppley BL, Woodell JE, Higgins J. Platelet quantification and growth factor analysis from platelet-rich plasma: implications for wound healing. Plast Reconstr Surg. 2004;114:1502–8.PubMedCrossRefGoogle Scholar
  16. 16.
    Kakudo N, Kushida S, Ogura T, Hara T, Suzuki K, Kusumoto K. The use of autologous platelet-rich plasma in the treatment of intractable skin ulcer: a case series. OJRM. 2012;1:29–32.CrossRefGoogle Scholar
  17. 17.
    Vang SN, Brady CP, Christensen KA, Allen KR, Anderson JE, Isler JR, Holt DW, Smith LM. Autologous platelet gel in coronary artery bypass grafting: effects on surgical wound healing. J Extra Corpor Technol. 2007;39:31–8.PubMedGoogle Scholar
  18. 18.
    Hom DB, Linzie BM, Huang TC. The healing effects of autologous platelet gel on acute human skin wounds. Arch Facial Plast Surg. 2007;9:174–83.PubMedCrossRefGoogle Scholar
  19. 19.
    Zavadil DP, Satterlee CC, Costigan JM, Holt DW, Shostrom VK. Autologous platelet gel and platelet-poor plasma reduce pain with total shoulder arthroplasty. J Extra Corpor Technol. 2007;39:177–82.PubMedGoogle Scholar
  20. 20.
    Brady C, Vang S, Christensen K, Isler J, Vollstedt K, Holt D. Use of autologous platelet gel in bariatric surgery. J Extra Corpor Technol. 2006;38:161–4.PubMedGoogle Scholar
  21. 21.
    Sclafani AP. Applications of platelet-rich fibrin matrix in facial plastic surgery. Facial Plast Surg. 2009;25:270–6.PubMedCrossRefGoogle Scholar
  22. 22.
    Sclafani AP. Platelet-rich fibrin matrix for improvement of deep nasolabial folds. J Cosmet Dermatol. 2010;9:66–71.PubMedCrossRefGoogle Scholar
  23. 23.
    Sclafani AP, McCormick SA. Induction of dermal collagenesis, angiogenesis, and adipogenesis in human skin by injection of platelet-rich fibrin matrix. Arch Facial Plast Surg. 2012;14:132–6.PubMedCrossRefGoogle Scholar

Copyright information

© The Japanese Society for Artificial Organs 2014

Authors and Affiliations

  • Satoshi Kushida
    • 1
    Email author
  • Natsuko Kakudo
    • 1
    Email author
  • Naoki Morimoto
    • 1
  • Tomoya Hara
    • 1
  • Takeshi Ogawa
    • 1
  • Toshihito Mitsui
    • 1
  • Kenji Kusumoto
    • 1
  1. 1.Department of Plastic and Reconstructive SurgeryKansai Medical UniversityHirakataJapan

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