Skip to main content
Log in

Development of biotube vascular grafts incorporating cuffs for easy implantation

  • Published:
Journal of Artificial Organs Aims and scope Submit manuscript


We are developing functional autologous tubular tissues, called biotubes, as ideal small-caliber vascular grafts that have growth potential without immunological rejection. In this study, to improve surgical handling, a novel biotube reinforced at the anastomosis regions was designed. Silicone rods, 2 mm in diameter and used as a mold, were covered with two pieces of short polyurethane sponge tubing as anastomotic reinforcement cuffs at both ends. After the assembly was placed into dorsal subcutaneous pouches in rabbits for 1 month, seamless biotubes incorporating the cuffs were obtained. The interstices and surfaces of the cuffs were impregnated and covered with connective tissues similar to those of the biotubes, mainly consisting of collagen and fibroblasts. Since the tubular shape of both ends was rigidly maintained, end-to-end anastomosis by conventional microsurgery techniques between biotubes and native carotid arteries was very easy. In preliminary autoimplantation studies, angiographic observation of up to 2 months duration showed no formation of aneurysms or rupturing.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others


  1. Y Nakayama H Ishibashi-Ueda K Takamizawa (2004) ArticleTitleIn vivo tissue-engineered small-caliber arterial graft prosthesis consisting of autologous tissue (Biotube) Cell Transplant 13 439–449 Occurrence Handle15468686

    PubMed  Google Scholar 

  2. O Sakai K Kanda H Yaku Y Nakayama (2005) ArticleTitleTissue organization acceleration technology in vivo in regenerative medicine: a wing-attached rod as a novel scaffold for “biotube” grafts fabrication Artif Organs 29 737

    Google Scholar 

  3. O Sakai K Kanda H Yaku Y Nakayama (2005) ArticleTitleTissue organization acceleration technology in vivo in regenerative medicine: promotion of angiogenesis by control-released nicotine Artif Organs 29 781

    Google Scholar 

  4. K Hayashida K Kanda H Yaku Y Nakayama (2005) ArticleTitleDevelopment of “in body tissue-architectural” autologous heart valve (biovalve): scaffold design, preparation method and performance Artif Organs 29 733

    Google Scholar 

  5. O Sakai Y Nakayama Y Nemoto Y Okamoto T Watanabe K Kanda H Yaku (2005) ArticleTitleDevelopment of sutureless vascular connecting system for easy implantation of small-caliber artificial grafts J Artif Organs 8 119–124 Occurrence Handle16094517 Occurrence Handle10.1007/s10047-005-0294-z

    Article  PubMed  Google Scholar 

  6. PMN Werker M Kon (1997) ArticleTitleReview of facilitated approaches to vascular anastomosis surgery Ann Thorac Surg 63 S122–S127 Occurrence Handle9203616 Occurrence Handle10.1016/S0003-4975(97)00340-8 Occurrence Handle1:STN:280:ByiA2cjislM%3D

    Article  PubMed  CAS  Google Scholar 

  7. T Mizobuchi Y Sekine K Yasufuku T Fujisawa DS Wilkes (2004) ArticleTitleComparison of surgical procedures for vascular and airway anastomoses that utilize a modified non-suture external cuff technique for experimental lung transplantation in rats J Heart Lung Transplant 23 IssueID7 889–893 Occurrence Handle15261185 Occurrence Handle10.1016/j.healun.2003.06.009

    Article  PubMed  Google Scholar 

  8. JM Panneton LH Hollier JM Hofer (2004) ArticleTitleMulticenter randomized prospective trial comparing a pre-cuffed polytetrafluoroethylene graft to a vein-cuffed polytetrafluoroethylene graft for infragenicular arterial bypass Ann Vasc Surg 18 IssueID2 199–206 Occurrence Handle15253256 Occurrence Handle10.1007/s10016-004-0012-y

    Article  PubMed  Google Scholar 

  9. G Steinthorsson B Sumpio (1999) ArticleTitleClinical and biological relevance of vein cuff anastomosis Acta Chir Belg 99 IssueID6 282–288 Occurrence Handle10674129 Occurrence Handle1:STN:280:DC%2BD3c7jvFSrtg%3D%3D

    PubMed  CAS  Google Scholar 

  10. BD Ratner AS Hoffman FJ Schoen JE Lemons (Eds) (1996) Biomaterials science Academic Press New York

    Google Scholar 

  11. RJ Zdrahala IJ Zdrahala (1999) ArticleTitleBiomedical applications of polyurethanes: a review of past promises, present realities, and a vibrant future J Biomater Appl 14 IssueID1 67–90 Occurrence Handle10405885 Occurrence Handle1:CAS:528:DyaK1MXktlyms70%3D

    PubMed  CAS  Google Scholar 

  12. S Nishi Y Nakayama H Ishibashi-Ueda T Matsuda (2003) ArticleTitleOcclusion of experimental aneurysms with heparin-loaded, microporous stent grafts Neurosurgery 53 1397–1405 Occurrence Handle14633306 Occurrence Handle10.1227/01.NEU.0000093427.89827.12

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations


Corresponding authors

Correspondence to Keiichi Kanda MD, PhD or Yasuhide Nakayama PhD.

Additional information

Both K. Kanda and Y. Nakayama are the corresponding authors.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Watanabe, T., Kanda, K., Ishibashi-Ueda, H. et al. Development of biotube vascular grafts incorporating cuffs for easy implantation. J Artif Organs 10, 10–15 (2007).

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI:

Key words