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In Vitro Monitoring of Live Cardiomyocytes Dynamics by a Scanning Near Field Optical Microscope Setup

  • 5th International Conference on NEAR FIELD OPTICS and RELATED TECHNOLOGIES (NFO-5)
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Abstract

We describe an original scanning near field optical microscope setup developed to examine rhythmically beating cardiac myocytes fully immersed in culture media. Scans could be halted at any point to record localized contraction profiles. Contractions could be detected with high sub nanometric vertical sensitivity and changed shape dramatically within adjacent sub micron-sized areas. We believe that the spatial dependency of contractions arises because of system’s ability to resolve the dynamic behavior of individual sub membrane actin bundles. Our results, combining imaging and real time recording in localized areas, reveal a new, non-invasive method for studying sub micron morphological activity in live biological samples.

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Authors and Affiliations

  1. Frontier Research Program, The Institute of Physical and Chemical Research (RIKEN), Wako, Hirosawa 2-1, Saitama, 351-0198, Japan

    Ruggero Micheletto, Morgan Denyer, Martin Scholl, Ken Nakajima & Masahiko Hara

  2. Max-Planck Institute for Polymer Research, Ackermannweg 10, D-55128, Mainz, Germany

    Wolfgang Knoll

Authors
  1. Ruggero Micheletto
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  2. Morgan Denyer
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  3. Martin Scholl
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  4. Ken Nakajima
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  5. Masahiko Hara
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  6. Wolfgang Knoll
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Corresponding author

Correspondence to Ruggero Micheletto.

Additional information

This paper was originally presented at the 5th International Conference on NEAR FIELD OPTICS and RELATED TECHNLOGIES (NFO-5), which was held on December 6–10, 1998 at Coganoi Bay Hotel, Shirahama, Japan, in cooperation with the Japan Society of Applied Physics and Mombusho Grant-in Aid for Scientific Research on Priority Areas “Near-Field Nano-optics” Projects, sponsored by Japan Society for the Promotion of Science.

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Micheletto, R., Denyer, M., Scholl, M. et al. In Vitro Monitoring of Live Cardiomyocytes Dynamics by a Scanning Near Field Optical Microscope Setup. OPT REV 6, 268–271 (1999). https://doi.org/10.1007/s10043-999-0268-0

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  • DOI: https://doi.org/10.1007/s10043-999-0268-0

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