Abstract
We propose a computed tomography (CT) for the incoherent optical system based on a generalized analytic reconstruction method from discrete samples (GARDS), by which an object image defined in a continuous space is reconstructed from discrete images acquired through the imaging system. We apply this method to reconstruct depth structures of micro-specimens using conventional fluorescence microscopes. Also, we discuss the optimal sampling distance in the depth direction through a generalized singular value decomposition of a continuous-to-discrete imaging system. We next apply the GARDS-based CT method to a double-axis microscope, in which two microscopes are set up perpendicularly to cover a missing frequency band of each microscopic imaging system. We show simulation results which verify the effectiveness of our proposed method.
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An erratum to this article is available at http://dx.doi.org/10.1007/BF02931725.
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Kikuchi, S., Matsuya, A., Yamaguchi, M. et al. Microscopic Computed Tomography Based on Generalized Analytic Reconstruction from Discrete Samples. OPT REV 3, 22–28 (1996). https://doi.org/10.1007/s10043-996-0022-9
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DOI: https://doi.org/10.1007/s10043-996-0022-9